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ELISA (Enzyme-Linked Immunosorbent Assay)
General Methods & Techniques
Choi DH, Katakura Y, Matsuda R, Hayashi Y, Hirobe M, Goda Y,
Ninomiya K, Shioya S.
Validation of a method for predicting the
precision, limit of detection and range of quantitation in
competitive ELISA.
Anal Sci. 2007 Feb;23(2):215-8.
PMID: 17297235 [PubMed - indexed for MEDLINE]
Kumada Y, Katoh S, Imanaka H, Imamura K, Nakanishi K.
Development of a one-step ELISA method using an
affinity peptide tag specific to a hydrophilic polystyrene surface.
J Biotechnol. 2007 Jan
1;127(2):288-99. Epub 2006 Jul 21.
PMID: 16950537 [PubMed - indexed for MEDLINE]
Guirola M, Carmenate T, Menendez T, Alvarez A, Gonzalez S, Guillen
G.
Comparison of three ELISA protocols to measure
antibody responses elicited against serogroup C meningococcal
polysaccharide in mouse, monkey and human sera.
J Microbiol Methods. 2006
Apr;65(1):135-43. Epub 2005 Aug 24.
PMID: 16122825 [PubMed - indexed for MEDLINE]
Bobrovnik SA, Komisarenko SV, Ilyina LV.
Novel and simple ELISA-based method for antibody
affinity determination.
Ukr Biokhim Zh. 2005
Mar-Apr;77(2):169-74.
PMID: 16335253 [PubMed - indexed for MEDLINE]
Alamdari DH, Kostidou E, Paletas K, Sarigianni M, Konstas AG,
Karapiperidou A, Koliakos G.
High sensitivity enzyme-linked immunosorbent
assay (ELISA) method for measuring protein carbonyl in samples with
low amounts of protein.
Free Radic Biol Med. 2005 Nov
15;39(10):1362-7. Epub 2005 Aug 19.
PMID: 16257645 [PubMed - indexed for MEDLINE]
Hilali HM, Simpkins AN, Hill WD, Waller JL, Knight RA, Fagan SC.
Single slice method for quantification of
hemorrhagic transformation using direct ELISA.
Neurol Res. 2004 Jan;26(1):93-8.
PMID: 14977065 [PubMed - indexed for MEDLINE]
Giampaoli S, Facciabene A, Mennuni C.
A protocol to guide development of a sensitive
ELISA for canine erythropoietin.
Vet Clin Pathol. 2003;32(4):199-201.
PMID: 14655105 [PubMed - indexed for MEDLINE]
A simple method for the determination of
dissociation constants by displacement ELISA.
J Immunol Methods. 2002 Dec
15;270(2):155-62.
PMID: 12379321 [PubMed - indexed for MEDLINE]
An in-house ELISA method for quantification of
circulating tissue factor.
Malays J Pathol. 2002
Jun;24(1):45-51.
PMID: 16329555 [PubMed - indexed for MEDLINE]
Optimization of immunogold labeling TEM: an
ELISA-based method for evaluation of blocking agents for
quantitative detection of antigen.
J Histochem Cytochem. 2002
Jun;50(6):863-73.
PMID: 12019302 [PubMed - indexed for MEDLINE]
ELISA-based method for determining the affinity
of bivalent antibodies of two specificities in a mixture.
Ukr Biokhim Zh. 2000
May-Jun;72(3):133-41.
PMID: 11200468 [PubMed - indexed for MEDLINE]
Ramandeep, Dikshit KL, Raje M.
Optimization of immunogold labeling TEM. An
ELISA-based method for rapid and convenient simulation of processing
conditions for quantitative detection of antigen.
J Histochem Cytochem. 2001
Mar;49(3):355-68.
PMID: 11181739 [PubMed - indexed for MEDLINE]
A rapid method for estimating the binding of
ligands to ELISA microwells.
J Immunol Methods. 2000 Apr
21;238(1-2):143-50.
PMID: 10758244 [PubMed - indexed for MEDLINE]
Sittampalam GS, Smith WC, Miyakawa TW, Smith DR, McMorris C.
Application of experimental design techniques to
optimize a competitive ELISA.
J Immunol Methods. 1996 Apr
19;190(2):151-61.
PMID: 8621950 [PubMed - indexed for MEDLINE]
Fuchs H, Orberger G, Tauber R, Gessner R.
Direct calibration ELISA: a rapid method for the
simplified determination of association constants of unlabeled
biological molecules.
J Immunol Methods. 1995 Dec
27;188(2):197-208.
PMID: 8551048 [PubMed - indexed for MEDLINE]
Bosompem KM, Assoku RK, Nantulya VM.
Hydrogen peroxide destaining: a new method for
removing non-specific stains in nitrocellulose membrane-based
dot-ELISA for the detection of trypanosomes in tsetse flies (Glossina
spp.).
J Immunol Methods. 1995 Nov
16;187(1):23-31.
PMID: 7490455 [PubMed - indexed for MEDLINE]
Ballegaard M, Hunding A, Rubin I.
A reliable method to estimate the association
constant for a monoclonal antibody and a protein antigen by enzyme
linked immunosorbent assay, ELISA.
J Immunoassay. 1995 May;16(2):123-36.
PMID: 7629274 [PubMed - indexed for MEDLINE]
Method for determining the affinity of monoclonal
antibody using non-competitive ELISA: a computer program.
J Immunoassay. 1994 May;15(2):115-28.
PMID: 8040347 [PubMed - indexed for MEDLINE]
Guerin-Marchand C, Batard T, Brodard V, Desvaux FX, Senechal H,
David B, Peltre G.
DMISA (dissociated membrane immunosorbent assay),
a new ELISA technique performed with blotted samples.
J Immunol Methods. 1994 Jan
3;167(1-2):219-25.
PMID: 8308278 [PubMed - indexed for MEDLINE]
Abe M, Goto T, Wolfenbarger D, Weiss DT, Solomon A.
Novel immunization protocol and ELISA screening
methods used to obtain and characterize monoclonal antibodies
specific for human light chain variable-region subgroups.
Hybridoma. 1993 Aug;12(4):475-83.
PMID: 8244419 [PubMed - indexed for MEDLINE]
Intrathecal synthesis of specific antibodies in
neuroborreliosis. Comparison of different ELISA techniques and
calculation methods.
J Neurol Sci. 1993 Aug;118(1):64-72.
PMID: 8229052 [PubMed - indexed for MEDLINE]
Goldberg ME, Djavadi-Ohaniance L.
Methods for measurement of antibody/antigen
affinity based on ELISA and RIA.
Curr Opin Immunol. 1993
Apr;5(2):278-81. Review.
PMID: 8507406 [PubMed - indexed for MEDLINE]
Raghava GP, Joshi AK, Agrewala JN.
Calculation of antibody and antigen
concentrations from ELISA data using a graphical method.
J Immunol Methods. 1992 Aug
30;153(1-2):263-4.
PMID: 1517598 [PubMed - indexed for MEDLINE]
Ivanov VS, Suvorova ZK, Tchikin LD, Kozhich AT, Ivanov VT.
Effective method for synthetic peptide
immobilization that increases the sensitivity and specificity of
ELISA procedures.
J Immunol Methods. 1992 Aug
30;153(1-2):229-33.
PMID: 1517594 [PubMed - indexed for MEDLINE]
Madersbacher S, Wolf H, Gerth R, Berger P.
Increased ELISA sensitivity using a modified
method for conjugating horseradish peroxidase to monoclonal
antibodies.
J Immunol Methods. 1992 Jul
31;152(1):9-13.
PMID: 1379279 [PubMed - indexed for MEDLINE]
Evaluation of a simple method for improving the
precision of an ELISA detecting antibody in serum.
J Immunol Methods. 1992 Apr
8;148(1-2):57-64.
PMID: 1564335 [PubMed - indexed for MEDLINE]
Sithigorngul P, Stretton AO, Cowden C.
A versatile dot-ELISA method with femtomole
sensitivity for detecting small peptides.
J Immunol Methods. 1991 Jul
26;141(1):23-32.
PMID: 1865121 [PubMed - indexed for MEDLINE]
A fast and efficient method for quantification of
monoclonal antibodies in an ELISA using a novel incubation system.
J Immunol Methods. 1990 Jul
20;131(1):83-9.
PMID: 2199581 [PubMed - indexed for MEDLINE]
Bunch DS, Rocke DM, Harrison RO.
Statistical design of ELISA protocols.
J Immunol Methods. 1990 Sep
14;132(2):247-54.
PMID: 2212680 [PubMed - indexed for MEDLINE]
Presentini R, Perin F, Ancilli G, Boraschi D, Volpini G, Antoni G.
Influence of the peptide insolubilization method
on detection of anti-peptide antibodies in ELISA. Evaluation of
nonspecific interactions.
J Immunoassay. 1989;10(4):395-412.
PMID: 2606976 [PubMed - indexed for MEDLINE]
Schots A, Van der Leede BJ, De Jongh E, Egberts E.
A method for the determination of antibody
affinity using a direct ELISA.
J Immunol Methods. 1988 May
9;109(2):225-33.
PMID: 3361133 [PubMed - indexed for MEDLINE]
A method for the quantitative analysis of ELISA
data.
J Virol Methods. 1987
Feb;15(3):213-22.
PMID: 3558703 [PubMed - indexed for MEDLINE]
Franci C, Ingles J, Castro R, Vidal J.
Further studies on the ELISA-spot technique. Its
application to particulate antigens and a potential improvement in
sensitivity.
J Immunol Methods. 1986 Apr
17;88(2):225-32.
PMID: 3007626 [PubMed - indexed for MEDLINE]
Brennand DM, Danson MJ, Hough DW.
A comparison of ELISA screening methods for the
production of monoclonal antibodies against soluble protein
antigens.
J Immunol Methods. 1986 Oct
23;93(1):9-14.
PMID: 3772109 [PubMed - indexed for MEDLINE]
A cell ELISA technique. The direct detection and
semi-quantitation of immunoglobulin positive cells in 7 day
lymphocyte cultures using the microtitre culture plates as the solid
phase.
J Immunol Methods. 1983 Feb
25;57(1-3):37-42.
PMID: 6338126 [PubMed - indexed for MEDLINE]
An assessment of environmental contamination
arising from the use of equipment for carrying out the ELISA
technique in microtitre plates.
J Clin Pathol. 1983 Jan;36(1):110-3.
PMID: 6337186 [PubMed - indexed for MEDLINE]
Voller A, Bartlett A, Bidwell DE.
Enzyme immunoassays with special reference to
ELISA techniques.
J Clin Pathol. 1978 Jun;31(6):507-20.
Review.
PMID: 78929 [PubMed - indexed for MEDLINE]