Immuno-Electron Microscopy (IEM)

Antigen-Antibody Specific Applications




AA-type amyloid

Linke RP, Huhn D, Casanova S, Donini U.

Immunoelectron microscopic identification of human AA-type amyloid: exploration of various monoclonal AA-antibodies, methods of fixation, embedding and of other parameters for the protein-A gold method.
Lab Invest. 1989 Dec;61(6):691-7.
PMID: 2601301 [PubMed - indexed for MEDLINE]



Bendayan M.

Ultrastructural localization of actin in muscle, epithelial and secretory cells by applying the protein A-gold immunocytochemical technique.
Histochem J. 1983 Jan;15(1):39-58.
PMID: 6339443 [PubMed - indexed for MEDLINE]

Grzanka A, Grzanka D.

Distribution of actin in etoposide-induced human leukemia cell line K-562 using fluorescence and immunoelectron microscopy technique.
Pol J Pathol. 2002;53(2):43-50.
PMID: 12140866 [PubMed - indexed for MEDLINE]


Amyloid protein

Toyoda M, Kita S, Furiya K, Osamura Y.

Characterization of AL amyloid protein identified by immunoelectron microscopy: a simple method using the protein A-gold technique.
J Histochem Cytochem. 1991 Feb;39(2):239-42.
PMID: 1987268 [PubMed - indexed for MEDLINE]

Linke RP, Nathrath WB, Wilson PD.

Immuno-electron microscopic identification and classification of amyloid in tissue sections by the postembedding protein-A gold method.
Ultrastruct Pathol. 1983 Jan;4(1):1-7.
PMID: 6344373 [PubMed - indexed for MEDLINE]



Nemoto N, Kawaoi A, Shikata T.

Occurrence of alpha-fetoprotein-containing hepatocytes in human embryos and fetuses: an immunohistochemical study using the light and ultrastructural immunoperoxidase methods.
J Histochem Cytochem. 1982 Oct;30(10):1022-32.
PMID: 6182184 [PubMed - indexed for MEDLINE]

Kinoyama S, Yamada G, Okushin H, Mimura H, Kobayashi T, Tsuji T.

Immunoelectron microscopic observation of alpha-fetoprotein synthesis in human non-malignant liver tissues using immunoperoxidase methods.
Gastroenterol Jpn. 1988 Aug;23(4):414-22.
PMID: 2460391 [PubMed - indexed for MEDLINE]



Zimmer KP, Caselitz J, Seifert G, Grenner G.

Immunoelectron microscopy of amylase in the human parotid gland. Ultrastructural localization by use of both the protein A-gold and the biotin-avidin-gold technique.
Virchows Arch A Pathol Anat Histopathol. 1984;404(2):187-96.
PMID: 6207660 [PubMed - indexed for MEDLINE]


Basement membrane antigens

Masunaga T, Shimizu H, Ishiko A, Nishikawa T.

Evaluation of immunoelectron microscopic techniques in the study of basement membrane antigens.
Histochem Cell Biol. 1998 Aug;110(2):107-11.
PMID: 9720982 [PubMed - indexed for MEDLINE]


beta-oxidation enzymes

Yokota S, Hashimoto T.

Innermembrane association of three mitochondrial beta-oxidation enzymes revealed by immunoelectron microscopic technique.
Histochemistry. 1984;80(6):547-52.
PMID: 6469712 [PubMed - indexed for MEDLINE]


Birch-pollen antigens

Grote M, Fromme HG.

Immunoelectron-microscopic localization of diffusible birch-pollen antigens in ultrathin sections using the protein-A/gold technique.
Histochemistry. 1984;81(5):489-92.
PMID: 6520024 [PubMed - indexed for MEDLINE]



Zabel M.

Ultrastructural localization of calcitonin in control and stimulated thyroid C cells of the rat using protein A-gold immunocytochemical technique.
Histochemistry. 1983;77(2):269-73.
PMID: 6841144 [PubMed - indexed for MEDLINE]

Dammrich J, Ormanns W, Schaffer R.

Electron microscopic demonstration of calcitonin in human medullary carcinoma of thyroid by the immuno gold staining method.
Histochemistry. 1984;81(4):369-72.
PMID: 6511490 [PubMed - indexed for MEDLINE]


Cathepsin B

Yokota S, Tsuji H, Kato K.

Immunocytochemical localization of cathepsin B in rat kidney. II. Electron microscopic study using the protein A-gold technique.
J Histochem Cytochem. 1986 Jul;34(7):899-907.
PMID: 3519753 [PubMed - indexed for MEDLINE]


Complement C3

Maciejewski W, Braun-Falco O, Scherer R.

Immunoelectron-microscopical localization of in vivo-bound complement C3 in bullous pemphigoid with the use of the peroxidase-antiperoxidase multistep technique.
Arch Dermatol Res. 1979 Oct;266(2):205-11.
PMID: 393183 [PubMed - indexed for MEDLINE]



Anandacoomaraswamy KS, Dutton N, Rajan GP, Eikelboom RH, Atlas MD, Robertson T.

Utilization of fresh human tympanic membranes for structural analysis and cytokeratin immunocytochemistry implementing resin techniques.
Acta Otolaryngol. 2006 Feb;126(2):149-53.
PMID: 16428191 [PubMed - indexed for MEDLINE]


Cytoskeletal proteins

Loesser KE, Doane KJ, Wilson FJ, Roisen FJ, Malamed S.

Improved immunoelectron microscopic method for localizing cytoskeletal proteins in Lowicryl K4M embedded tissues.
J Histochem Cytochem. 1986 Nov;34(11):1477-85.
PMID: 3534078 [PubMed - indexed for MEDLINE]


Flagellar (H) and somatic (O) antigens

Yamaguchi M, Kondo I.

Immunoelectron microscopy of Proteus vulgaris by the plasma polymerization metal-extraction replica method: differential staining of flagellar (H) and somatic (O) antigens by colloidal golds.
J Electron Microsc (Tokyo). 1989;38(5):382-8.
PMID: 2479701 [PubMed - indexed for MEDLINE]



Iijima K, Ohtomo K, Kobayashi R, Kojima N.

Immunohistochemical studies on the GABAergic system in the rat supraoptic nucleus using the PAP method with an application of electron microscopy.
Arch Histol Jpn. 1986 Dec;49(5):579-91.
PMID: 3551872 [PubMed - indexed for MEDLINE]

Hansen GH, Hosli E, Belhage B, Schousboe A, Hosli L.

Light and electron microscopic localization of GABAA-receptors on cultured cerebellar granule cells and astrocytes using immunohistochemical techniques.
Neurochem Res. 1991 Mar;16(3):341-6.
PMID: 1664060 [PubMed - indexed for MEDLINE]

Romijn HJ, Janszen AW, Pool CW, Buijs RM.

An improved immunocytochemical staining method for large semi-thin plastic epon sections: application to GABA in rat cerebral cortex.
J Histochem Cytochem. 1993 Aug;41(8):1259-65.
PMID: 7687264 [PubMed - indexed for MEDLINE]

Maxwell DJ, Christie WM, Somogyi P.

Synaptic connections of GABA-containing boutons in the lateral cervical nucleus of the cat: an ultrastructural study employing pre- and post-embedding immunocytochemical methods.
Neuroscience. 1989;33(1):169-84.
PMID: 2601854 [PubMed - indexed for MEDLINE]


Glucose-6-phosphate dehydrogenase

Ninfali P, Biagiotti E, Guidi L, Malatesta M, Gazzanelli G, Del Grande P.

Cytochemical and immunocytochemical methods for electron microscopic detection of glucose-6-phosphate dehydrogenase in brain areas.
Brain Res Brain Res Protoc. 2000 Feb;5(1):115-20.
PMID: 10719273 [PubMed - indexed for MEDLINE]


Glutathione peroxidase

Asayama K, Yokota S, Dobashi K, Hayashibe H, Kawaoi A, Nakazawa S.

Purification and immunoelectron microscopic localization of cellular glutathione peroxidase in rat hepatocytes: quantitative analysis by postembedding method.
Histochemistry. 1994 Sep;102(3):213-9.
PMID: 7868363 [PubMed - indexed for MEDLINE]


Glycoprotein hormones

Tougard C, Picart R, Tixier-Vidal A.

Immunocytochemical localization of glycoprotein hormones in the rat anterior pituitary. A light and electron microscope study using antisera against rat bet subunits: a comparison between preembedding and postembeeding methods.
J Histochem Cytochem. 1980 Feb;28(2):101-14.
PMID: 6986430 [PubMed - indexed for MEDLINE]



Suzukawa K, Kawaoi A, Sato K, Ishii S.

Quantitative analysis of immunoreactive human growth hormone (HGH) in the secretory granules of normal pituitary glands and hgh-producing pituitary adenomas. An immunoelectron microscopic study using the protein A-gold (PAG) technique.
Acta Pathol Jpn. 1988 Feb;38(2):153-62.
PMID: 2839004 [PubMed - indexed for MEDLINE]



Login GR, Galli SJ, Dvorak AM.

Immunocytochemical localization of histamine in secretory granules of rat peritoneal mast cells with conventional or rapid microwave fixation and an ultrastructural post-embedding immunogold technique.
J Histochem Cytochem. 1992 Sep;40(9):1247-56.
PMID: 1506663 [PubMed - indexed for MEDLINE]


Ia-like antigen

Feng-Chen KC, Chen BF, Liu Z, Ng AK.

Application of avidin-biotin-peroxidase complex method with a monoclonal antibody to human Ia-like antigen in immunoelectron microscopy.
J Histochem Cytochem. 1986 Nov;34(11):1495-9.
PMID: 3534079 [PubMed - indexed for MEDLINE]



Viale G, Dell'Orto P, Braidotti P, Coggi G.

Ultrastructural localization of intracellular immunoglobulins in Epon-embedded human lymph nodes. An immunoelectron microscopic investigation using the immunogold staining (IGS) and the avidin-biotin-peroxidase complex (ABC) methods.
J Histochem Cytochem. 1985 May;33(5):400-6.
PMID: 2580880 [PubMed - indexed for MEDLINE]



Saccomanno K, Taccagni G, Bosi E, Preti P, Dozio N, Cantaboni A.

Immunoelectron microscopy: a new method for detection of insulin antibodies.
J Histochem Cytochem. 1993 Aug;41(8):1233-9.
PMID: 8331287 [PubMed - indexed for MEDLINE]


Keratin filaments

Hanna W, Andrighetti L.

Immunoelectron microscopic localization of keratin filaments: a post-embedding technique.
J Cutan Pathol. 1988 Aug;15(4):223-5.
PMID: 2460513 [PubMed - indexed for MEDLINE]



Charpin C, Kopp F, Pourreau-Schneider N, Lissitzky JC, Lavaut MN, Martin PM, Toga M.

Laminin distribution in human decidua and immature placenta. An immunoelectron microscopic study (avidin-biotin-peroxidase complex method).
Am J Obstet Gynecol. 1985 Mar 15;151(6):822-6.
PMID: 3883788 [PubMed - indexed for MEDLINE]


Lysosomal and peroxisomal enzymes

Yokota S, Tsuji H, Kato K.

Localization of lysosomal and peroxisomal enzymes in the specific granules of rat intestinal eosinophil leukocytes revealed by immunoelectron microscopic techniques.
J Histochem Cytochem. 1984 Mar;32(3):267-74.
PMID: 6693757 [PubMed - indexed for MEDLINE]



Sato N, Morita T, Ishiwata I, Nakai M, Kami K.

Cellular characterization of the 91kDa-ectopic ascitic antigen sharing antigenicity with MRP8 in the human placenta as revealed by immunoelectron microscopical colloidal-gold techniques.
Okajimas Folia Anat Jpn. 1998 Mar;74(6):217-29.
PMID: 9584513 [PubMed - indexed for MEDLINE]



Kozlowski GP, Frenk S, Brownfield MS.

Localization of neurophysin in the rat supraoptic nucleus. I. Ultrastructural immunocytochemistry using the post-embedding technique.
Cell Tissue Res. 1977 Apr 29;179(4):467-73.
PMID: 324631 [PubMed - indexed for MEDLINE]



Beaudet A, Dournaud P, Boudin H.

Complementarity of radioautographic and immunohistochemical techniques for localizing neuroreceptors at the light and electron microscopy level.
Braz J Med Biol Res. 1998 Feb;31(2):215-23. Review.
PMID: 9686144 [PubMed - indexed for MEDLINE]


Nitric oxide synthase

Stanarius A, Uckert S, Machtens SA, Stief CG, Wolf G, Jonas U.

Immunocytochemical distribution of nitric oxide synthase in the human corpus cavernosum: an electron microscopical study using the tyramide signal amplification technique.
Urol Res. 2001 Jun;29(3):168-72.
PMID: 11482440 [PubMed - indexed for MEDLINE]


Osteonectin and Gla-protein

Bianco P, Hayashi Y, Silvestrini G, Termine JD, Bonucci E.

Osteonectin and Gla-protein in calf bone: ultrastructural immunohistochemical localization using the Protein A-gold method.
Calcif Tissue Int. 1985 Dec;37(6):684-6.
PMID: 2418932 [PubMed - indexed for MEDLINE]



Osamura RY.

Processing of proopiomelanocortin (POMC) approached by immunoelectron microscopy pre-embedding method.
Pathol Res Pract. 1991 Jun;187(5):543-5.
PMID: 1656407 [PubMed - indexed for MEDLINE]


Procollagens. II

Karim A, Cournil I, Leblond CP.

Immunohistochemical localization of procollagens. II. Electron microscopic distribution of procollagen I antigenicity in the odontoblasts and predentin of rat incisor teeth by a direct method using peroxidase linked antibodies.
J Histochem Cytochem. 1979 Jul;27(7):1070-83.
PMID: 89154 [PubMed - indexed for MEDLINE]


Prolactin, growth hormone and luteinizing hormone

Dacheux F, Dubois MP.

Ultrastructural localization of prolactin, growth hormone and luteinizing hormone by immunocytochemical techniques in the bovine pituitary.
Cell Tissue Res. 1976 Oct 29;174(2):245-60.
PMID: 793722 [PubMed - indexed for MEDLINE]



Tanner VA, Ploug T, Tao-Cheng JH.

Subcellular localization of SV2 and other secretory vesicle components in PC12 cells by an efficient method of preembedding EM immunocytochemistry for cell cultures.
J Histochem Cytochem. 1996 Dec;44(12):1481-8.
PMID: 8985140 [PubMed - indexed for MEDLINE]


Tamm-Horsfall glycoprotein

Sikri KL, Foster CL, Alexander DP, Marshall RD.

Localization of Tamm-Horsfall glycoprotein in the fetal and neonatal hamster kidney as demonstrated by immunofluorescence and immunoelectron microscopical techniques.
Biol Neonate. 1981;39(5-6):305-12.
PMID: 7020781 [PubMed - indexed for MEDLINE]

Sikri KL, Foster CL, MacHugh N, Marshall RD.

Localization of Tamm-Horsfall glycoprotein in the human kidney using immuno-fluorescence and immuno-electron microscopical techniques.
J Anat. 1981 Jun;132(Pt 4):597-605.
PMID: 7028707 [PubMed - indexed for MEDLINE]


Tyrosine hydroxylase and substance P

Feher E, Fodor M, Donath T.

Electron microscopic immunocytochemical evidence for the synaptic connections between tyrosine hydroxylase and substance P containing nerve elements in the coeliac ganglion of cat revealed by a double labelling technique.
Acta Biol Hung. 1996;47(1-4):119-27.
PMID: 9123985 [PubMed - indexed for MEDLINE]



Wilson FJ, Hirabayashi T, Cheng M.

Distribution of antibodies to the troponin complex, troponin-C and troponin-I in chicken skeletal muscle as determined by a simplified method for immuno-electron microscopy.
Tissue Cell. 1977;9(2):273-84.
PMID: 906017 [PubMed - indexed for MEDLINE]


Virus-induced surface antigens

Morris RE, Fritz RB.

Ultrastructural visualization of virus-induced surface antigens. Comparative studies of three immunohistochemical techniques.
J Histochem Cytochem. 1975 Nov;23(11):855-62.
PMID: 172555 [PubMed - indexed for MEDLINE]



Thomsen E, Hansen BL, Hansen GN, Jensen PV.

Ultrastructural immunocytochemical localization of vitellogenin in the fat body of the blowfly, Calliphora vicina Rob.-Desv. (erythrocephala Meig.) by use of the unlabeled antibody-enzyme method.
Cell Tissue Res. 1980;208(3):445-55.
PMID: 6994888 [PubMed - indexed for MEDLINE]