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Immuno-Electron Microscopy (IEM)
General Methods & Techniques
Takizawa T, Anderson CL, Robinson JM.
A new method to enhance contrast of ultrathin
cryosections for immunoelectron microscopy.
J Histochem Cytochem. 2003 Jan;51(1):31-9.
PMID: 12502752 [PubMed - indexed for MEDLINE]
A novel embedment-free immunoelectron microscopy
technique reveals association of apoptosis-regulating proteins with
subcellular structures.
Histochem J. 2002 Aug-Sep;34(8-9):441-6.
PMID: 12814192 [PubMed - indexed for MEDLINE]
Trombitas K, Pollack GH, Greaser ML.
Adaptation of a super-sensitive epitope detection
technique for the immunoelectron microscopy of titin filaments in
vertebrate striated muscle.
J Microsc. 1999 Dec;196(Pt 3):299-304.
PMID: 10594770 [PubMed - indexed for MEDLINE]
Muller-Hocker J, Schafer S, Sendelhofert A, Weis S.
The application of a biotin-anti-biotin gold
technique providing a significant signal intensification in electron
microscopic immunocytochemistry: a comparison with the ultrasmall
immunogold silver staining procedure.
Histochem Cell Biol. 1998 Feb;109(2):119-25.
PMID: 9504772 [PubMed - indexed for MEDLINE]
Improved technique for immunoelectron microscopy.
How to prepare epoxy resin to obtain approximately the same
immunogold labeling for epoxy sections as for acrylic sections
without any etching.
Micron. 1996 Jun-Aug;27(3-4):211-7.
PMID: 8953240 [PubMed - indexed for MEDLINE]
Microwave fixation method
for cytochemistry. For conventional electron microscopy,
enzymo-immunocytochemistry, autoradiography elemental distribution
studies and staining methods.
Eur J Morphol. 1996 Dec;34(5):385-91.
PMID: 9034795 [PubMed - indexed for MEDLINE]
Phend KD, Rustioni A, Weinberg RJ.
An osmium-free method of epon embedment that
preserves both ultrastructure and antigenicity for post-embedding
immunocytochemistry.
J Histochem Cytochem. 1995 Mar;43(3):283-92.
PMID: 7532656 [PubMed - indexed for MEDLINE]
Schaumburg-Lever G, Fehrenbacher B, Moller H, Nau P.
Techniques in immuno-electron microscopy. I.
Cryosubstitution.
J Cutan Pathol. 1994 Aug;21(4):330-8.
PMID: 7798389 [PubMed - indexed for MEDLINE]
Combined immunocytochemistry and enzyme
cytochemistry on ultra-thin cryosections: a new method.
J Histochem Cytochem. 1993 Nov;41(11):1635-9.
PMID: 8409371 [PubMed - indexed for MEDLINE]
Prins FA, van Diemen-Steenvoorde R, Bonnet J, Cornelese-ten Velde I.
Reflection contrast
microscopy of ultrathin sections in immunocytochemical localization
studies: a versatile technique bridging electron microscopy with
light microscopy.
Histochemistry. 1993 Jun;99(6):417-25.
PMID: 8407367 [PubMed - indexed for MEDLINE]
Silver-enhanced diaminobenzidine-sulfide (SEDS):
a technique for high-resolution immunoelectron microscopy
demonstrated with monoamine immunoreactivity in monkey cerebral
cortex and caudate.
J Histochem Cytochem. 1993 Sep;41(9):1393-404.
PMID: 8354879 [PubMed - indexed for MEDLINE]
de Mesy Jensen KL, di Sant'Agnese PA.
Large block embedding and "pop-off" technique for
immunoelectron microscopy.
Ultrastruct Pathol. 1992 Jan-Apr;16(1-2):51-9.
PMID: 1557830 [PubMed - indexed for MEDLINE]
Incubation chambers for the staining of ultrathin
sections. An in situ technique especially suited for
immunocytochemistry.
Acta Histochem. 1992;93(2):353-6.
PMID: 1283798 [PubMed - indexed for MEDLINE]
Unfixed tissue for electron immunocytochemistry:
a simple preparation method for colloidal gold localization of
sensitive epitopes using ethanediol dehydration.
Histochem J. 1992 Apr;24(4):190-206. Erratum in: Histochem J 1992
Oct;24(10):757.
PMID: 1375208 [PubMed - indexed for MEDLINE]
An easy method for orientated embedding tissue
culture cell monolayers in LR White resin for postembedding
immunocytochemistry.
J Electron Microsc Tech. 1991 Apr;17(4):456-8.
PMID: 1865243 [PubMed - indexed for MEDLINE]
Stierhof YD, Humbel BM, Schwarz H.
Suitability of different silver enhancement
methods applied to 1 nm colloidal gold particles: an immunoelectron
microscopic study.
J Electron Microsc Tech. 1991 Mar;17(3):336-43.
PMID: 1646315 [PubMed - indexed for MEDLINE]
Tatsumi H, Suzuki K, Senda T, Satoh S, Fujita H, Taniguchi N.
Post-embedding immuno-electron microscopy with
rapid freezing and freeze-substitution techniques for the
localization of manganese superoxide dismutase.
Arch Histol Cytol. 1991 Oct;54(4):465-9.
PMID: 1760224 [PubMed - indexed for MEDLINE]
Immuno- and affinity probes
for electron microscopy: a review of labeling and preparation
techniques.
J Histochem Cytochem. 1990 Feb;38(2):145-57. Review.
PMID: 2405054 [PubMed - indexed for MEDLINE]
A neutral pH silver development method for the
visualization of 1-nanometer gold particles in pre-embedding
electron microscopic immunocytochemistry.
J Histochem Cytochem. 1990 Apr;38(4):503-8. Erratum in: J Histochem
Cytochem 1990 Sep;38(9):1396.
PMID: 2319121 [PubMed - indexed for MEDLINE]
An improved flat embedding technique for
immunoelectron microscopy.
Stain Technol. 1989 May;64(3):143-6.
PMID: 2480005 [PubMed - indexed for MEDLINE]
A new method of the
immunohistochemical detection of cellular antigens for light and
electron microscopy.
Histochemistry. 1989;91(6):449-54.
PMID: 2475460 [PubMed - indexed for MEDLINE]
McPhail GD, Finn T, Isaacson PG.
A useful low temperature
method for post-embedding electron immunocytochemistry in routine
histopathology.
J Pathol. 1987 Mar;151(3):231-8.
PMID: 3553511 [PubMed - indexed for MEDLINE]
Linner JG, Livesey SA, Harrison DS, Steiner AL.
A new technique for removal of amorphous phase
tissue water without ice crystal damage: a preparative method for
ultrastructural analysis and immunoelectron microscopy.
J Histochem Cytochem. 1986 Sep;34(9):1123-35.
PMID: 2426340 [PubMed - indexed for MEDLINE]
Serial semithin sections in immunohistochemistry:
techniques and applications.
Arch Histol Jpn. 1986 Oct;49(4):391-410. Review.
PMID: 3545131 [PubMed - indexed for MEDLINE]
A low-temperature embedding colloidal gold
technique for immunoelectron microscopy.
Diagn Immunol. 1986;4(1):47-55.
PMID: 3514072 [PubMed - indexed for MEDLINE]
Nagele RG, Kosciuk MC, Wang SM, Spero DA, Lee H.
A method for preparing
quick-frozen, freeze-substituted cells for transmission electron
microscopy and immunocytochemistry.
J Microsc. 1985 Sep;139(Pt 3):291-301.
PMID: 3935796 [PubMed - indexed for MEDLINE]
Hisano S, Adachi T, Daikoku S.
Freeze-drying technique in electron microscopic
immunohistochemistry.
J Histochem Cytochem. 1985 May;33(5):485-90.
PMID: 3989275 [PubMed - indexed for MEDLINE]
Marotta L, Shero M, Carter JM, Klohs W, Apicella MA.
Radio frequency glow discharge and solid-phase
lactoperoxidase-glucose oxidase beads as methods for etching
ultra-thin plastic sections for immunoelectron microscopy.
J Immunol Methods. 1984 Jun 8;71(1):69-82.
PMID: 6202797 [PubMed - indexed for MEDLINE]
The protein A-gold technique. Use in the
immunoelectron microscopic study of the teleost growth hormone.
J UOEH. 1984 Mar 1;6(1):39-46.
PMID: 6382525 [PubMed - indexed for MEDLINE]
Eldred WD, Zucker C, Karten HJ, Yazulla S.
Comparison of fixation and
penetration enhancement techniques for use in ultrastructural
immunocytochemistry.
J Histochem Cytochem. 1983 Feb;31(2):285-92.
PMID: 6339606 [PubMed - indexed for MEDLINE]
An alternative fixation-processing method for
preembedding ultrastructural immunocytochemistry of cytoplasmic
antigens: the GBS (glutaraldehyde-borohydride-saponin) procedure.
J Histochem Cytochem. 1983 Jun;31(6):791-8.
PMID: 6404984 [PubMed - indexed for MEDLINE]
A simple reliable method for producing electron
dense markers of uniform size for use in immunoelectron microscopy.
J Immunol Methods. 1982 Mar 26;49(3):237-46.
PMID: 6802900 [PubMed - indexed for MEDLINE]
Trost TH, Steigleder GK, Bodeux E.
Immuno-electron
microscopical features of in vivo antibody binding during pemphigus
blister formation. Revelation with a new technique: peroxidase-labeled
protein A.
Arch Dermatol Res. 1981;270(4):463-8.
PMID: 7283474 [PubMed - indexed for MEDLINE]
An improved method for the
collection of serial cells for electron microscopic analysis of
their immunocytochemical stain.
J Histochem Cytochem. 1980 Mar;28(3):279-81.
PMID: 6243684 [PubMed - indexed for MEDLINE]
Dutton AH, Tokuyasu KT, Singer SJ.
Iron-dextran antibody conjugates: General method
for simultaneous staining of two components in high-resolution
immunoelectron microscopy.
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3392-3396.
PMID: 16592679 [PubMed - as supplied by publisher]
A simple technique for supporting single cells in
electron microscopic immunocytochemistry and embedding.
Histochemistry. 1977 Oct 3;54(1):51-6.
PMID: 199561 [PubMed - indexed for MEDLINE]
A streamlined technique for
on-grid electron immunocytochemistry.
Histochemistry. 1977 Aug 22;53(3):271-2.
PMID: 334710 [PubMed - indexed for MEDLINE]