Fluorescence Activated Cell Sorting (FACS)

Tissue-Cell Specific Applications





Chen WY, Lu L, McDonald K, Osmond DG, Smith CE.

Isolation of amelogenin-positive ameloblasts from rat mandibular incisor enamel organs by flow cytometry and fluorescence activated cell sorting.
Connect Tissue Res. 1998;38(1-4):9-15; discussion 35-41.
PMID: 11063012 [PubMed - indexed for MEDLINE]



Degenhart HJ, van der Maarel JW, Jongkind H, Raatgeep HC, Neijens HJ.

Properties of human basophils isolated by fluorescence-activated cell sorting.
J Allergy Clin Immunol. 1988 Sep;82(3 Pt 1):455-61.
PMID: 2459176 [PubMed - indexed for MEDLINE]


B Cell

Tung JW, Parks DR, Moore WA, Herzenberg LA, Herzenberg LA.

Identification of B-cell subsets: an exposition of 11-color (Hi-D) FACS methods.
Methods Mol Biol. 2004;271:37-58.
PMID: 15146111 [PubMed - indexed for MEDLINE]

Hoven MY, De Leij L, Keij JF, The TH.

Detection and isolation of antigen-specific B cells by the fluorescence activated cell sorter (FACS).
J Immunol Methods. 1989 Feb 24;117(2):275-84.
PMID: 2784157 [PubMed - indexed for MEDLINE]

Osmond DG, Melchers F, Paige CJ.

Pre-B cells in mouse bone marrow: in vitro maturation of peanut agglutinin binding B lymphocyte precursors separated from bone marrow by fluorescence-activated cell sorting.
J Immunol. 1984 Jul;133(1):86-90.
PMID: 6427348 [PubMed - indexed for MEDLINE]


beta Cell of Pancreas

de Krijger RR, Aanstoot HJ, Kranenburg G, Verkerk A, Jongkind JF, van Strik R, Lafferty KJ, Bruining GJ.

Enrichment of beta cells from the human fetal pancreas by fluorescence activated cell sorting with a new monoclonal antibody.
Diabetologia. 1992 May;35(5):436-43.
PMID: 1521725 [PubMed - indexed for MEDLINE]


Dendritic Cell

Ni K, O'Neill HC.

Improved FACS analysis confirms generation of immature dendritic cells in long-term stromal-dependent spleen cultures.
Immunol Cell Biol. 2000 Jun;78(3):196-204.
PMID: 10849107 [PubMed - indexed for MEDLINE]

Crowley M, Inaba K, Witmer-Pack M, Steinman RM.

The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus.
Cell Immunol. 1989 Jan;118(1):108-25.
PMID: 2910499 [PubMed - indexed for MEDLINE]


Endothelial Cell

Visser MJ, van Lennep AC, van Bockel JH, van Hinsbergh VW, van der Keur M, Hermans J.

Evaluation of various methods to quantify endothelial cells attached to vascular prostheses: comparison with a new "gold standard" FACS method.
J Surg Res. 1996 Feb 15;61(1):237-43.
PMID: 8769972 [PubMed - indexed for MEDLINE]

Westphal JR, Boerbooms AM, Schalwijk CJ, Kwast H, De Weijert M, Jacobs C, Vierwinden G, Ruiter DJ, Van de Putte LB, De Waal RM.

Anti-endothelial cell antibodies in sera of patients with autoimmune diseases: comparison between ELISA and FACS analysis.
Clin Exp Immunol. 1994 Jun;96(3):444-9.
PMID: 8004814 [PubMed - indexed for MEDLINE]


Epithelial Cell

Smalley MJ, Titley J, O'Hare MJ.

Clonal characterization of mouse mammary luminal epithelial and myoepithelial cells separated by fluorescence-activated cell sorting.
In Vitro Cell Dev Biol Anim. 1998 Oct;34(9):711-21.
PMID: 9794223 [PubMed - indexed for MEDLINE]


Fetal Cell

Herzenberg LA, Bianchi DW, Schroder J, Cann HM, Iverson GM.

Fetal cells in the blood of pregnant women: detection and enrichment by fluorescence-activated cell sorting.
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1453-5.
PMID: 286330 [PubMed - indexed for MEDLINE]


Germ Cell

Mozdziak PE, Angerman-Stewart J, Rushton B, Pardue SL, Petitte JN.

Isolation of chicken primordial germ cells using fluorescence-activated cell sorting.
Poult Sci. 2005 Apr;84(4):594-600.
PMID: 15844816 [PubMed - indexed for MEDLINE]


Haemopoietic Cell

Swirsky DM, Watt SM, Gilmore DJ, Hayhoe FG, Waldmann H.

The characterisation of monoclonal antibodies against haemopoietic cells: comparison of an immunoperoxidase method with fluorescence activated cell sorting.
J Immunol Methods. 1983 Jul 15;61(2):171-82.
PMID: 6345676 [PubMed - indexed for MEDLINE]


Hepatic progenitor cell

Fujikawa T, Hirose T, Fujii H, Oe S, Yasuchika K, Azuma H, Yamaoka Y.

Purification of adult hepatic progenitor cells using green fluorescent protein (GFP)-transgenic mice and fluorescence-activated cell sorting.
J Hepatol. 2003 Aug;39(2):162-70.
PMID: 12873811 [PubMed - indexed for MEDLINE]



Sigal SH, Brill S, Reid LM, Zvibel I, Gupta S, Hixson D, Faris R, Holst PA.

Characterization and enrichment of fetal rat hepatoblasts by immunoadsorption ("panning") and fluorescence-activated cell sorting.
Hepatology. 1994 Apr;19(4):999-1006.
PMID: 7511129 [PubMed - indexed for MEDLINE]


Hybrid and Transfected Cell

Kamarck ME.

Fluorescence-activated cell sorting of hybrid and transfected cells.
Methods Enzymol. 1987;151:150-65.
PMID: 3431439 [PubMed - indexed for MEDLINE]


Islet Cell

Jiao L, Gray DW, Gohde W, Flynn GJ, Morris PJ.

In vitro staining of islets of Langerhans for fluorescence-activated cell sorting.
Transplantation. 1991 Sep;52(3):450-2.
PMID: 1716795 [PubMed - indexed for MEDLINE]

Nielsen DA, Lernmark A, Berelowitz M, Bloom GD, Steiner DF.

Sorting of pancreatic islet cell subpopulations by light scattering using a fluorescence-activated cell sorter.
Diabetes. 1982 Apr;31(4 Pt 1):299-306.
PMID: 6130019 [PubMed - indexed for MEDLINE]


Lymphoblastoid Cell

McFarland RD, Vincent JL, Smith GJ.

Isolation by fluorescence-activated cell sorting of cells of a human lymphoblastoid cell strain containing mutations in the lambda immunoglobulin gene.
Environ Mol Mutagen. 1992;19(4):297-303.
PMID: 1600956 [PubMed - indexed for MEDLINE]



Carter J, Newport A, Keeler KD, Dresser DW.

FACS analysis of changes in T and B lymphocyte populations in the blood, spleen and lymph nodes of pregnant mice.
Immunology. 1983 Apr;48(4):791-7.
PMID: 6601050 [PubMed - indexed for MEDLINE]


Macroglial Cell

Williams BP, Abney ER, Raff MC.

Macroglial cell development in embryonic rat brain: studies using monoclonal antibodies, fluorescence activated cell sorting, and cell culture.
Dev Biol. 1985 Nov;112(1):126-34.
PMID: 4054431 [PubMed - indexed for MEDLINE]



Auerbach HS, Lalande ME, Latt S, Colten HR.

Isolation of guinea pig macrophages bearing surface C4 by fluorescence-activated cell sorting: correlation between surface C4 antigen and C4 protein secretion.
J Immunol. 1983 Nov;131(5):2420-6.
PMID: 6355294 [PubMed - indexed for MEDLINE]



Tomer A, Harker LA, Burstein SA.

Purification of human megakaryocytes by fluorescence-activated cell sorting.
Blood. 1987 Dec;70(6):1735-42.
PMID: 3315039 [PubMed - indexed for MEDLINE]


Melanoma Cell

Dantas ME, Brown JP, Thomas MR, Robinson WA, Glode LM.

Detection of melanoma cells in bone marrow using monoclonal antibodies. A comparison of fluorescence activated cell sorting (FACS) and conventional immunofluorescence (IF).
Cancer. 1983 Sep 15;52(6):949-53.
PMID: 6349783 [PubMed - indexed for MEDLINE]



Schaffner AE, St John PA, Barker JL.

Fluorescence-activated cell sorting of embryonic mouse and rat motoneurons and their long-term survival in vitro.
J Neurosci. 1987 Oct;7(10):3088-104.
PMID: 2822863 [PubMed - indexed for MEDLINE]


Neuronal Cell

Sergent-Tanguy S, Chagneau C, Neveu I, Naveilhan P.

Fluorescent activated cell sorting (FACS): a rapid and reliable method to estimate the number of neurons in a mixed population.
J Neurosci Methods. 2003 Oct 15;129(1):73-9.
PMID: 12951234 [PubMed - indexed for MEDLINE]

Asami M, Nakatsuka T, Hayashi T, Kou K, Kagawa H, Agata K.

Cultivation and characterization of planarian neuronal cells isolated by fluorescence activated cell sorting (FACS).
Zoolog Sci. 2002 Nov;19(11):1257-65.
PMID: 12499670 [PubMed - indexed for MEDLINE]

Asami M, Nakatsuka T, Hayashi T, Kou K, Kagawa H, Agata K.

Cultivation and characterization of planarian neuronal cells isolated by fluorescence activated cell sorting (FACS).
Zoolog Sci. 2002 Nov;19(11):1257-65.
PMID: 12499670 [PubMed - indexed for MEDLINE]

St John PA, Kell WM, Mazzetta JS, Lange GD, Barker JL.

Analysis and isolation of embryonic mammalian neurons by fluorescence-activated cell sorting.
J Neurosci. 1986 May;6(5):1492-512.
PMID: 2872281 [PubMed - indexed for MEDLINE]



Abney ER, Williams BP, Raff MC.

Tracing the development of oligodendrocytes from precursor cells using monoclonal antibodies, fluorescence-activated cell sorting, and cell culture.
Dev Biol. 1983 Nov;100(1):166-71.
PMID: 6194025 [PubMed - indexed for MEDLINE]


Osteoblast-like Cell

Tsuru S, Kitani H, Oguchi M, Mashiko M, Zinnaka Y, Shimomura Y.

Separation of osteoblast-like cells from bone marrow by fluorescence-activated cell sorting.
J Histochem Cytochem. 1984 Jan;32(1):43-8.
PMID: 6317742 [PubMed - indexed for MEDLINE]


Ovary Cell

Zou CP, Van NT, Kuo MT.

Isolation and characterization of putative intrinsic multidrug resistant Chinese hamster ovary cells by fluorescence activated cell sorting.
Anticancer Res. 1992 Mar-Apr;12(2):427-32.
PMID: 1349795 [PubMed - indexed for MEDLINE]

Cain CC, Wilson RB, Murphy RF.

Isolation by fluorescence-activated cell sorting of Chinese hamster ovary cell lines with pleiotropic, temperature-conditional defects in receptor recycling.
J Biol Chem. 1991 Jun 25;266(18):11746-52.
PMID: 1711039 [PubMed - indexed for MEDLINE]


Purkinje Cell

Tomomura M, Rice DS, Morgan JI, Yuzaki M.

Purification of Purkinje cells by fluorescence-activated cell sorting from transgenic mice that express green fluorescent protein.
Eur J Neurosci. 2001 Jul;14(1):57-63.
PMID: 11488949 [PubMed - indexed for MEDLINE]


Rabbit Cell

Cinader B, Dubiski S, Chou CT, Charpentier L.

Fluorescence-activated cell sorter (FACS) analysis of rabbit cells.
Cell Immunol. 1988 Apr 1;112(2):293-301.
PMID: 2451567 [PubMed - indexed for MEDLINE]



Rathjen FG, Holcombe B, Gierer A.

Separation of cell subpopulations from embryonic chick neural retina with fluorescence-activated cell sorting.
J Biochem Biophys Methods. 1981 Mar;4(3-4):191-207.
PMID: 7240648 [PubMed - indexed for MEDLINE]


Stem Cell

Hewitt Z, Forsyth NR, Waterfall M, Wojtacha D, Thomson AJ, McWhir J.

Fluorescence-activated single cell sorting of human embryonic stem cells.
Cloning Stem Cells. 2006 Fall;8(3):225-34.
PMID: 17009898 [PubMed - indexed for MEDLINE]

Lin HT, Chiou SH, Kao CL, Shyr YM, Hsu CJ, Tarng YW, Ho LL, Kwok CF, Ku HH.

Characterization of pancreatic stem cells derived from adult human pancreas ducts by fluorescence activated cell sorting.
World J Gastroenterol. 2006 Jul 28;12(28):4529-35.
PMID: 16874866 [PubMed - indexed for MEDLINE]

Hayashi T, Asami M, Higuchi S, Shibata N, Agata K.

Isolation of planarian X-ray-sensitive stem cells by fluorescence-activated cell sorting.
Dev Growth Differ. 2006 Aug;48(6):371-80.
PMID: 16872450 [PubMed - indexed for MEDLINE]

Baines P, Visser JW.

Analysis and separation of murine bone marrow stem cells by H33342 fluorescence-activated cell sorting.
Exp Hematol. 1983 Sep;11(8):701-8.
PMID: 6195006 [PubMed - indexed for MEDLINE]

Visser JW, Bol SJ, van den Engh G.

Characterization and enrichment of murine hemopoietic stem cells by fluorescence activated cell sorting.
Exp Hematol. 1981 Jul;9(6):644-55.
PMID: 6167457 [PubMed - indexed for MEDLINE]



Gift EA, Park HJ, Paradis GA, Demain AL, Weaver JC.

FACS-based isolation of slowly growing cells: double encapsulation of yeast in gel microdrops.
Nat Biotechnol. 1996 Jul;14(7):884-7.
PMID: 9631016 [PubMed - indexed for MEDLINE]