Flow Cytometry (FC)

Antigen-Antibody Specific Applications




ABO blood group antibodies

Stussi G, Huggel K, Lutz HU, Schanz U, Rieben R, Seebach JD.

Isotype-specific detection of ABO blood group antibodies using a novel flow cytometric method.
Br J Haematol. 2005 Sep;130(6):954-63.
PMID: 16156865 [PubMed - indexed for MEDLINE]


Adenosine Deaminase

SenGupta S, Petsche D, Gelfand EW, Chechik BE.

A flow cytometric method for the detection of adenosine deaminase in mononuclear cells.
J Immunol Methods. 1985 Jun 25;80(2):155-62.
PMID: 3925020 [PubMed - indexed for MEDLINE]


Alkaline phosphatase

Agrawal YP, Ylikangas P, Parviainen MT, Penttila IM.

Flow cytometric method to measure urea-resistant alkaline phosphatase from blood neutrophils for use in the prenatal screening of Down's syndrome.
Prenat Diagn. 1994 Dec;14(12):1141-9.
PMID: 7899281 [PubMed - indexed for MEDLINE]


Annexin V

Goldberg JE, Sherwood SW, Clayberger C.

A novel method for measuring CTL and NK cell-mediated cytotoxicity using annexin V and two-color flow cytometry.
J Immunol Methods. 1999 Apr 22;224(1-2):1-9.
PMID: 10357200 [PubMed - indexed for MEDLINE]


Basophil surface antigens

Bochner BS, McKelvey AA, Schleimer RP, Hildreth JE, MacGlashan DW Jr.

Flow cytometric methods for the analysis of human basophil surface antigens and viability.
J Immunol Methods. 1989 Dec 20;125(1-2):265-71.
PMID: 2607157 [PubMed - indexed for MEDLINE]


B-Cell antibodies

Berteli AJ, Daniel V, Terness P, Opelz G.

A new method for determining anti-B cell antibodies and their specificity using flow cytometry.
J Immunol Methods. 1993 Aug 26;164(1):21-5.
PMID: 8360506 [PubMed - indexed for MEDLINE]



Zamai L, Canonico B, Gritzapis A, Luchetti F, Felici C, Della Felice M, Margomenou L, Papamichail M, Gratama JW, Papa S.

Intracellular detection of Bcl-2 and p53 proteins by flow cytometry: comparison of monoclonal antibodies and sample preparation protocols.
J Biol Regul Homeost Agents. 2002 Oct-Dec;16(4):289-302.
PMID: 12597645 [PubMed - indexed for MEDLINE]



Mouawad R, Khayat D, Zerrouqi A, Ghoumari AM, Soubrane C.

A flow cytometric method for the rapid detection of beta-galactosidase transfected cells: an in vitro and in vivo study.
J Immunol Methods. 1997 May 12;204(1):51-6.
PMID: 9202709 [PubMed - indexed for MEDLINE]



Eidukevicius R, Characiejus D, Janavicius R, Kazlauskaite N, Pasukoniene V, Mauricas M, Den Otter W.

A method to estimate cell cycle time and growth fraction using bromodeoxyuridine-flow cytometry data from a single sample.
BMC Cancer. 2005 Sep 22;5:122. Erratum in: BMC Cancer. 2006;6:184.
PMID: 16176590 [PubMed - indexed for MEDLINE]

Schutte B, Reynders MM, van Assche CL, Hupperets PS, Bosman FT, Blijham GH.

An improved method for the immunocytochemical detection of bromodeoxyuridine labeled nuclei using flow cytometry.
Cytometry. 1987 Jul;8(4):372-6.
PMID: 3113895 [PubMed - indexed for MEDLINE]

Orfao A, Carbajo S, Dolbeare F, Ciudad J, Lopez A, Carbajo-Perez E.

Comparison of Vindelov et al. and bromodeoxyuridine/DNA double-staining flow cytometry methods for analysis of cell cycle distribution in rat thymocytes.
Cytometry. 1996 Sep 1;25(1):104-8.
PMID: 8875060 [PubMed - indexed for MEDLINE]

Kubbies M, Schindler D, Hoehn H, Rabinovitch PS.

Cell cycle kinetics by BrdU-Hoechst flow cytometry: an alternative to the differential metaphase labelling technique.
Cell Tissue Kinet. 1985 Sep;18(5):551-62.
PMID: 2411405 [PubMed - indexed for MEDLINE]

Kroll W.

Modification of the BrdU/H33258 technique for flow cytometry based on the pH-dependence of the fluorescence of H33258 stained DNA.
Histochemistry. 1984;80(5):493-6.
PMID: 6207150 [PubMed - indexed for MEDLINE]

Holm M, Thomsen M, Hoyer M, Hokland P.

Optimization of a flow cytometric method for the simultaneous measurement of cell surface antigen, DNA content, and in vitro BrdUrd incorporation into normal and malignant hematopoietic cells.
Cytometry. 1998 May 1;32(1):28-36.
PMID: 9581621 [PubMed - indexed for MEDLINE]

White RA, Terry NH.

A quantitative method for evaluating bivariate flow cytometric data obtained using monoclonal antibodies to bromodeoxyuridine.
Cytometry. 1992;13(5):490-5.
PMID: 1633728 [PubMed - indexed for MEDLINE]

van Erp PE, Brons PP, Boezeman JB, de Jongh GJ, Bauer FW.

A rapid flow cytometric method for bivariate bromodeoxyuridine/DNA analysis using simultaneous proteolytic enzyme digestion and acid denaturation.
Cytometry. 1988 Nov;9(6):627-30.
PMID: 2463135 [PubMed - indexed for MEDLINE]

Giese G, Kubbies M, Traub P.

High resolution analysis of cell cycle-correlated vimentin expression in asynchronously grown, TPA-treated MPC-11 cells by the novel flow cytometric multiparameter BrdU-Hoechst/PI and immunolabeling technique.
J Cell Physiol. 1994 Nov;161(2):209-16.
PMID: 7525610 [PubMed - indexed for MEDLINE]



Eksioglu-Demiralp E, Kitada S, Carson D, Garland J, Andreef M, Reed JC.

A method for functional evaluation of caspase activation pathways in intact lymphoid cells using electroporation-mediated protein delivery and flow cytometric analysis.
J Immunol Methods. 2003 Apr 1;275(1-2):41-56.
PMID: 12667669 [PubMed - indexed for MEDLINE]



Barbesti S, Soldini L, Carcelain G, Guignet A, Colizzi V, Mantelli B, Corvaglia A, Tran-Minh T, Dorigatti F, Autran B, Lazzarin A, Beretta A.

A simplified flow cytometry method of CD4 and CD8 cell counting based on thermoresistant reagents: implications for large scale monitoring of HIV-infected patients in resource-limited settings.
Cytometry B Clin Cytom. 2005 Nov;68(1):43-51.
PMID: 16184613 [PubMed - indexed for MEDLINE]

Abella N, Schelcher F, Delverdier M, Concordet D, Valarcher JF, Espinasse J, Cabanie P.

Flow cytometric analysis of bovine CD4 and CD8 lymphocytes: influence of blood sampling and processing methods.
Res Vet Sci. 1994 Sep;57(2):163-71.
PMID: 7817005 [PubMed - indexed for MEDLINE]

Alexander TS.

Absolute CD4 counts obtained by a three-color flow-cytometric method without the use of a hematology analyzer.
Clin Diagn Lab Immunol. 1998 Mar;5(2):266-9.
PMID: 9521157 [PubMed - indexed for MEDLINE]



Carlsson L, Ronquist G, Eliasson R, Egberg N, Larsson A.

Flow cytometric technique for determination of prostasomal quantity, size and expression of CD10, CD13, CD26 and CD59 in human seminal plasma.
Int J Androl. 2006 Apr;29(2):331-8.
PMID: 16533355 [PubMed - indexed for MEDLINE]



Brocklebank AM, Sparrow RL.

Enumeration of CD34+ cells in cord blood: a variation on a single-platform flow cytometric method based on the ISHAGE gating strategy.
Cytometry. 2001 Aug 15;46(4):254-61.
PMID: 11514960 [PubMed - indexed for MEDLINE]

Humpe A, Beck C, Schoch R, Kneba M, Horst HA.

Establishment and optimization of a flow cytometric method for evaluation of viability of CD34+ cells after cryopreservation and comparison with trypan blue exclusion staining.
Transfusion. 2005 Jul;45(7):1208-13.
PMID: 15987368 [PubMed - indexed for MEDLINE]



Hernandez-Campo PM, Martin-Ayuso M, Almeida J, Lopez A, Orfao A.

Comparative analysis of different flow cytometry-based immunophenotypic methods for the analysis of CD59 and CD55 expression on major peripheral blood cell subsets.
Cytometry. 2002 Jun 15;50(3):191-201.
PMID: 12116342 [PubMed - indexed for MEDLINE]

Oelschlaegel U, Besson I, Arnoulet C, Sainty D, Nowak R, Naumann R, Bux Y, Ehninger G.

A standardized flow cytometric method for screening paroxysmal nocturnal haemoglobinuria (PNH) measuring CD55 and CD59 expression on erythrocytes and granulocytes.
Clin Lab Haematol. 2001 Apr;23(2):81-90.
PMID: 11488846 [PubMed - indexed for MEDLINE]



Frezzolini A, Provini A, Teofoli P, Pomponi D, De Pita O.

Serum-induced basophil CD63 expression by means of a tricolour flow cytometric method for the in vitro diagnosis of chronic urticaria.
Allergy. 2006 Sep;61(9):1071-7.
PMID: 16918509 [PubMed - indexed for MEDLINE]

Monneret G, Gutowski MC, Bienvenu J.

Detection of allergen-induced basophil activation by expression of CD63 antigen using a tricolour flow cytometric method.
Clin Exp Immunol. 1999 Mar;115(3):393-6.
PMID: 10193408 [PubMed - indexed for MEDLINE]



Simms PE, Ellis TM.

Utility of flow cytometric detection of CD69 expression as a rapid method for determining poly- and oligoclonal lymphocyte activation.
Clin Diagn Lab Immunol. 1996 May;3(3):301-4.
PMID: 8705673 [PubMed - indexed for MEDLINE]



Torous DK, Hall NE, Illi-Love AH, Diehl MS, Cederbrant K, Sandelin K, Ponten I, Bolcsfoldi G, Ferguson LR, Pearson A, Majeska JB, Tarca JP, Hynes GM, Lynch AM, McNamee JP, Bellier PV, Parenteau M, Blakey D, Bayley J, van der Leede BJ, Vanparys P, Harbach PR, Zhao S, Filipunas AL, Johnson CW, Tometsko CR, Dertinger SD.

Interlaboratory validation of a CD71-based flow cytometric method (Microflow) for the scoring of micronucleated reticulocytes in mouse peripheral blood.
Environ Mol Mutagen. 2005;45(1):44-55.
PMID: 15605355 [PubMed - indexed for MEDLINE]

Woolley IJ, Wood EM, Sramkoski RM, Zimmerman PA, Miller JP, Kazura JW.

Expression of Duffy antigen receptor for chemokines during reticulocyte maturation: using a CD71 flow cytometric technique to identify reticulocytes.
Immunohematol. 2005;21(1):15-20.
PMID: 15783301 [PubMed - indexed for MEDLINE]


Chorionic Gonadotropin

Acevedo HF, Krichevsky A, Campbell-Acevedo EA, Galyon JC, Buffo MJ, Hartsock RJ.

Flow cytometry method for the analysis of membrane-associated human chorionic gonadotropin, its subunits, and fragments on human cancer cells.
Cancer. 1992 Apr 1;69(7):1818-28.
PMID: 1372527 [PubMed - indexed for MEDLINE]



Siennicka J.

Comparison of different protocols of MRC5 cell line permeabilization for detection of CMV IE antigens by flow cytometry.
Acta Microbiol Pol. 2000;49(2):167-9.
PMID: 11093679 [PubMed - indexed for MEDLINE]


CR1 complement receptors

Cohen JH, Aubry JP, Jouvin MH, Wijdenes J, Bancherau J, Kazatchkine M, Revillard JP.

Enumeration of CR1 complement receptors on erythrocytes using a new method for detecting low density cell surface antigens by flow cytometry.
J Immunol Methods. 1987 May 4;99(1):53-8.
PMID: 2952733 [PubMed - indexed for MEDLINE]



Ruitenberg JJ, Waters CA.

A rapid flow cytometric method for the detection of intracellular cyclooxygenases in human whole blood monocytes and a COX-2 inducible human cell line.
J Immunol Methods. 2003 Mar 1;274(1-2):93-104.
PMID: 12609536 [PubMed - indexed for MEDLINE]


Cytochrome C

Campos CB, Paim BA, Cosso RG, Castilho RF, Rottenberg H, Vercesi AE.

Method for monitoring of mitochondrial cytochrome c release during cell death: Immunodetection of cytochrome c by flow cytometry after selective permeabilization of the plasma membrane.
Cytometry A. 2006 Jun;69(6):515-23.
PMID: 16680678 [PubMed - indexed for MEDLINE]



Schuerwegh AJ, De Clerck LS, Bridts CH, Stevens WJ.

Comparison of intracellular cytokine production with extracellular cytokine levels using two flow cytometric techniques.
Cytometry B Clin Cytom. 2003 Sep;55(1):52-8.
PMID: 12949960 [PubMed - indexed for MEDLINE]

Jason J, Larned J.

Single-cell cytokine profiles in normal humans: comparison of flow cytometric reagents and stimulation protocols.
J Immunol Methods. 1997 Aug 22;207(1):13-22.
PMID: 9328582 [PubMed - indexed for MEDLINE]



Brotherick I, Lennard TW, Wilkinson SE, Cook S, Angus B, Shenton BK.

Flow cytometric method for the measurement of epidermal growth factor receptor and comparison with the radio-ligand binding assay.
Cytometry. 1994 Jul 1;16(3):262-9.
PMID: 7924696 [PubMed - indexed for MEDLINE]


Erythrocyte membrane antigen

Pattanapanyasat K, Webster HK, Udomsangpetch R, Wanachiwanawin W, Yongvanitchit K.

Flow cytometric two-color staining technique for simultaneous determination of human erythrocyte membrane antigen and intracellular malarial DNA.
Cytometry. 1992;13(2):182-7.
PMID: 1372210 [PubMed - indexed for MEDLINE]


Factor X

Le Guyader A, Davis-Gorman G, Copeland JG, McDonagh PF.

A flow cytometric method for determining the binding of coagulation factor X to monocytes in whole human blood.
J Immunol Methods. 2004 Sep;292(1-2):207-15.
PMID: 15350525 [PubMed - indexed for MEDLINE]


Fc Function

Ramasamy I, Tran E, Charnock A, Farrugia A.

Flow-cytometric method for the quantitation of the Fc function of intravenous immunoglobulin preparations.
Vox Sang. 2000;78(3):185-93.
PMID: 10838520 [PubMed - indexed for MEDLINE]


Feto-maternal haemorrhage

Bromilow IM, Duguid JK.

Measurement of feto-maternal haemorrhage: a comparative study of three Kleihauer techniques and tow flow cytometry methods.
Clin Lab Haematol. 1997 Jun;19(2):137-42.
PMID: 9218154 [PubMed - indexed for MEDLINE]



Kantakamalakul W, Jaroenpool J, Pattanapanyasat K.

A novel enhanced green fluorescent protein (EGFP)-K562 flow cytometric method for measuring natural killer (NK) cell cytotoxic activity.
J Immunol Methods. 2003 Jan 15;272(1-2):189-97.
PMID: 12505723 [PubMed - indexed for MEDLINE]


GM1 antibodies

Escande-Beillard N, David MJ, Portoukalian J, Pouget J, Azulay JP, Bernard D, Boucraut J.

A sensitive flow cytometry method for anti-GM1 antibodies detection.
J Neuroimmunol. 2002 Apr;125(1-2):163-9.
PMID: 11960653 [PubMed - indexed for MEDLINE]



Waller A, Simons PC, Biggs SM, Edwards BS, Prossnitz ER, Sklar LA.

Techniques: GPCR assembly, pharmacology and screening by flow cytometry.
Trends Pharmacol Sci. 2004 Dec;25(12):663-9. Review.
PMID: 15530645 [PubMed - indexed for MEDLINE]

Schneider E, Mayer M, Ziemek R, Li L, Hutzler C, Bernhardt G, Buschauer A.

A simple and powerful flow cytometric method for the simultaneous determination of multiple parameters at G protein-coupled receptor subtypes.
Chembiochem. 2006 Sep;7(9):1400-9.
PMID: 16888730 [PubMed - indexed for MEDLINE]


Histone Acetylation

Ronzoni S, Faretta M, Ballarini M, Pelicci P, Minucci S.
New method to detect histone acetylation levels by flow cytometry.
Cytometry A. 2005 Jul;66(1):52-61.
PMID: 15915507 [PubMed - indexed for MEDLINE]



Schumm M, Feuchtinger T, Pfeiffer M, Hoelle W, Bethge W, Ebinger M, Kuci S, Handgretinger R, Lang P.

Flow cytometry with anti HLA-antibodies: a simple but highly sensitive method for monitoring chimerism and minimal residual disease after HLA-mismatched stem cell transplantation.
Bone Marrow Transplant. 2007 Jun;39(12):767-73. Epub 2007 Apr 16.
PMID: 17438586 [PubMed - in process]

Hulstaert F, Albrecht J, Hannet I, Lancaster P, Buchner L, Kunz J, Falkenrodt A, Tongio M, De Keyser F, Veys EM, et al.

An optimized method for routine HLA-B27 screening using flow cytometry.
Cytometry. 1994 Mar 15;18(1):21-9.
PMID: 8082484 [PubMed - indexed for MEDLINE]

Freedman J, Hornstein A.

Simple method for differentiating between HLA and platelet-specific antibodies by flow cytometry.
Am J Hematol. 1991 Dec;38(4):314-20.
PMID: 1746540 [PubMed - indexed for MEDLINE]

Worthington JE, Robson AJ, Sheldon S, Langton A, Martin S.

A comparison of enzyme-linked immunoabsorbent assays and flow cytometry techniques for the detection of HLA specific antibodies.
Hum Immunol. 2001 Oct;62(10):1178-84.
PMID: 11600227 [PubMed - indexed for MEDLINE]

Finck ME, Elmenkouri N, Debard AL, Bohe J, Lepape A, Bienvenu J, Monneret G.

Preliminary results in standardization of flow cytometry protocols for monocytic HLA-DR measurement and their application in the follow up of septic shock
Ann Biol Clin (Paris). 2003 Jul-Aug;61(4):441-8. French.
PMID: 12915353 [PubMed - indexed for MEDLINE]

Janssen WC, Rouwen JA, Hoffmann JJ.

Improved flow cytometric method for HLA-B27 typing.
Ann Clin Biochem. 1992 Nov;29 ( Pt 6):663-7.
PMID: 1489163 [PubMed - indexed for MEDLINE]

Piazza A, Poggi E, Ozzella G, Borrelli L, Monaco PI, Scornajenghi A, Tisone G, Adorno D.

Public epitope specificity of HLA class I antibodies induced by a failed kidney transplant: alloantibody characterization by flow cytometric techniques.
Transplantation. 2006 May 15;81(9):1298-305.
PMID: 16699458 [PubMed - indexed for MEDLINE]


Homing receptors

Wimazal F, Ghannadan M, Muller MR, End A, Willheim M, Meidlinger P, Schernthaner GH, Jordan JH, Hagen W, Agis H, Sperr WR, Czerwenka K, Lechner K, Valent P.

Expression of homing receptors and related molecules on human mast cells and basophils: a comparative analysis using multi-color flow cytometry and toluidine blue/immunofluorescence staining techniques.
Tissue Antigens. 1999 Nov;54(5):499-507.
PMID: 10599889 [PubMed - indexed for MEDLINE]


HPA 1a

Killie MK, Kjeldsen-Kragh J, Randen I, Skogen B, Husebekk A.

Evaluation of a new flow cytometric HPA 1a screening method. A rapid and reliable tool for HPA 1a screening of blood donors and pregnant women.
Transfus Apher Sci. 2004 Apr;30(2):89-92.
PMID: 15062744 [PubMed - indexed for MEDLINE]



Bachelet M, Mariethoz E, Banzet N, Souil E, Pinot F, Polla CZ, Durand P, Bouchaert I, Polla BS.

Flow cytometry is a rapid and reliable method for evaluating heat shock protein 70 expression in human monocytes.
Cell Stress Chaperones. 1998 Sep;3(3):168-76. Erratum in: Cell Stress Chaperones 1998 Dec;3(4):273.
PMID: 9764757 [PubMed - indexed for MEDLINE]

Cronje MJ, Snyman M, Bornman L, Weir IE.

A rapid and reliable flow cytometric method for determining Hsp70 levels in tobacco protoplasts.
Methods Cell Sci. 2003;25(3-4):237-46.
PMID: 15801170 [PubMed - indexed for MEDLINE]


IL-2 Receptors

Cohen N, Gumbert M, Birnbaum J, Lim VL, Falco D, Ascher N, Roberts J, Garovoy MR.

An improved method for the detection of soluble interleukin 2 receptors in liver transplant recipients by flow cytometry.
Transplantation. 1991 Feb;51(2):417-21.
PMID: 1899733 [PubMed - indexed for MEDLINE]

De Jong MO, Rozemuller H, Bauman JG, Visser JW.

Biotinylation of interleukin-2 (IL-2) for flow cytometric analysis of IL-2 receptor expression. Comparison of different methods.
J Immunol Methods. 1995 Jul 17;184(1):101-12.
PMID: 7622862 [PubMed - indexed for MEDLINE]


Interferon receptor IFNAR2

Tochizawa S, Akamatsu S, Sugiyama Y, Muraguchi M, Ohmoto Y, Ono Y, Ishikawa H, Tanigami A, Sumida T, Mori T.

A flow cytometric method for determination of the interferon receptor IFNAR2 subunit in peripheral blood leukocyte subsets.
J Pharmacol Toxicol Methods. 2004 Jul-Aug;50(1):59-66.
PMID: 15233969 [PubMed - indexed for MEDLINE]



Gorisse MC, Venteo L, Pluot M.

A method for simultaneous quantification of monoclonal antibody Ki-67 and DNA content by flow cytometry. Application to breast carcinomas.
Anal Quant Cytol Histol. 1999 Feb;21(1):8-16.
PMID: 10068769 [PubMed - indexed for MEDLINE]

Murakami T, Oga A, Uchiyama T, Kimura Y, Morimoto H, Suzuki T, Takahashi M.

A simple method for flow cytometric measurement of Ki-67 reactive antigen on small solid tissues
Gan To Kagaku Ryoho. 1993 Apr;20(6):737-40. Japanese.
PMID: 7683864 [PubMed - indexed for MEDLINE]

Endl E, Steinbach P, Knuchel R, Hofstadter F.

Analysis of cell cycle-related Ki-67 and p120 expression by flow cytometric BrdUrd-Hoechst/7AAD and immunolabeling technique.
Cytometry. 1997 Nov 1;29(3):233-41.
PMID: 9389440 [PubMed - indexed for MEDLINE]



Heath F, Jensen TG, Hansen PS, Jensen LG, Vissinger H, Moestrup SK, Pedersen S, Kolvraa S, Bolund L, Faergeman O.

A flow cytometric competition technique for measuring interaction of LDL with cellular LDL-receptors applied to patients with mutant (Arg3500-->Gln) apolipoprotein B.
Z Gastroenterol. 1996 Jun;34 Suppl 3:21-4.
PMID: 8767450 [PubMed - indexed for MEDLINE]


Lipoprotein receptor

Lohne K, Urdal P, Leren TP, Tonstad S, Ose L.

Standardization of a flow cytometric method for measurement of low-density lipoprotein receptor activity on blood mononuclear cells.
Cytometry. 1995 Aug 1;20(4):290-5.
PMID: 7587716 [PubMed - indexed for MEDLINE]


Lymphocytotoxic antibodies

Talbot D, Shenton BK, Givan AL, Proud G, Taylor RM.

A rapid, objective method for the detection of lymphocytotoxic antibodies using flow cytometry.
J Immunol Methods. 1987 May 4;99(1):137-40.
PMID: 3571990 [PubMed - indexed for MEDLINE]



Davey HM.

Flow cytometric techniques for the detection of microorganisms.
Methods Cell Sci. 2002;24(1-3):91-7. Review.
PMID: 12815297 [PubMed - indexed for MEDLINE]



Mancini M, Sedghinasab M, Knowlton K, Tam A, Hockenbery D, Anderson BO.

Flow cytometric measurement of mitochondrial mass and function: a novel method for assessing chemoresistance.
Ann Surg Oncol. 1998 Apr-May;5(3):287-95.
PMID: 9607633 [PubMed - indexed for MEDLINE]


Myeloperoxidase and Lysozyme

Lanza F, Latorraca A, Moretti S, Castagnari B, Ferrari L, Castoldi G.

Comparative analysis of different permeabilization methods for the flow cytometry measurement of cytoplasmic myeloperoxidase and lysozyme in normal and leukemic cells.
Cytometry. 1997 Jun 15;30(3):134-44.
PMID: 9222099 [PubMed - indexed for MEDLINE]

Imamura N.

Sensitive detection technique of myeloperoxidase precursor protein by flow cytometry with monoclonal antibodies.
Am J Hematol. 1998 Jul;58(3):241-3.
PMID: 9662278 [PubMed - indexed for MEDLINE]

Kappelmayer J, Gratama JW, Karaszi E, Menendez P, Ciudad J, Rivas R, Orfao A.

Flow cytometric detection of intracellular myeloperoxidase, CD3 and CD79a. Interaction between monoclonal antibody clones, fluorochromes and sample preparation protocols.
J Immunol Methods. 2000 Aug 28;242(1-2):53-65.
PMID: 10986389 [PubMed - indexed for MEDLINE]


Nuclear Factor-kappa B (NFkappaB)

Cognasse F, Sabido O, Beniguel L, Genin C, Garraud O.

A flow cytometry technique to study nuclear factor-kappa B (NFkappaB) translocation during human B cell activation.
Immunol Lett. 2003 Nov 15;90(1):49-52.
PMID: 14611907 [PubMed - indexed for MEDLINE]

Foulds S.

Novel flow cytometric method for quantifying nuclear binding of the transcription factor nuclear factor kappa B in unseparated human monocytes and polymorphonuclear cells.
Cytometry. 1997 Oct 1;29(2):182-6.
PMID: 9332826 [PubMed - indexed for MEDLINE]


Nuclear Matrix Proteins

Dynlacht JR, Henthorn J, O'Nan C, Dunn ST, Story MD.

Flow cytometric analysis of nuclear matrix proteins: method and potential applications.
Cytometry. 1996 Aug 1;24(4):348-59.
PMID: 8866219 [PubMed - indexed for MEDLINE]


Orthoclone OKT3

Lim VL, Gumbert M, Garovoy MR.

A flow cytometric method for the detection of the development of antibody to Orthoclone OKT3.
J Immunol Methods. 1989 Jul 26;121(2):197-201.
PMID: 2788193 [PubMed - indexed for MEDLINE]



Morkve O, Hostmark J.

Influence of tissue preparation techniques on p53 expression in bronchial and bladder carcinomas, assessed by immunofluorescence staining and flow cytometry.
Cytometry. 1991;12(7):622-7.
PMID: 1782831 [PubMed - indexed for MEDLINE]

Filippini G, Griffin S, Uhr M, Eppenberger H, Bonilla J, Cavalli F, Soldati G.

A novel flow cytometric method for the quantification of p53 gene expression.
Cytometry. 1998 Mar 1;31(3):180-6.
PMID: 9515717 [PubMed - indexed for MEDLINE]



Kuhn U, Lempertz U, Knop J, Becker D.

A new method for phenotyping proliferating cell nuclear antigen positive cells using flow cytometry: implications for analysis of the immune response in vivo.
J Immunol Methods. 1995 Feb 27;179(2):215-22.
PMID: 7876569 [PubMed - indexed for MEDLINE]

Ichikawa A, Shimamura K, Koyama Y.

Flow cytometric techniques for measurement of proliferating cell nuclear antigen (PCNA)
Nippon Rinsho. 1992 Oct;50(10):2343-8. Japanese.
PMID: 1360029 [PubMed - indexed for MEDLINE]



Wang EJ, Casciano CN, Clement RP, Johnson WW.

In vitro flow cytometry method to quantitatively assess inhibitors of P-glycoprotein.
Drug Metab Dispos. 2000 May;28(5):522-8.
PMID: 10772630 [PubMed - indexed for MEDLINE]

Takeshita A, Shinjo K, Ohnishi K, Ohno R.

New flow cytometric method for detection of minimally expressed multidrug resistance P-glycoprotein on normal and acute leukemia cells using biotinylated MRK16 and streptavidin-RED670 conjugate.
Jpn J Cancer Res. 1995 Jun;86(6):607-15.
PMID: 7622426 [PubMed - indexed for MEDLINE]

Ferrand VL, Montero Julian FA, Chauvet MM, Hirn MH, Bourdeaux MJ.

Quantitative determination of the MDR-related P-glycoprotein, Pgp 170, by a rapid flow cytometric technique.
Cytometry. 1996 Feb 1;23(2):120-5.
PMID: 8742170 [PubMed - indexed for MEDLINE]


Poly (ADP-ribose)

Ogata S, Okumura K, Taguchi H.

A simple and rapid method for the detection of poly(ADP-ribose) by flow cytometry.
Biosci Biotechnol Biochem. 2000 Mar;64(3):510-5.
PMID: 10803947 [PubMed - indexed for MEDLINE]


Pyruvate dehydrogenase

Elkhalifa MY, Kiechle FL, Gordon SC, Chen J, Poulik MD.

A flow cytometric method to detect anti-pyruvate dehydrogenase antibody in primary biliary cirrhosis.
Am J Clin Pathol. 1992 Feb;97(2):202-8.
PMID: 1546688 [PubMed - indexed for MEDLINE]



Morice WG, Kimlinger T, Katzmann JA, Lust JA, Heimgartner PJ, Halling KC, Hanson CA.

Flow cytometric assessment of TCR-Vbeta expression in the evaluation of peripheral blood involvement by T-cell lymphoproliferative disorders: a comparison with conventional T-cell immunophenotyping and molecular genetic techniques.
Am J Clin Pathol. 2004 Mar;121(3):373-83.
PMID: 15023042 [PubMed - indexed for MEDLINE]



Horvatinovich JM, Sparks SD, Borowitz MJ.

Detection of terminal deoxynucleotidyl transferase by flow cytometry: a three color method.
Cytometry. 1994 Dec 15;18(4):228-30.
PMID: 7895530 [PubMed - indexed for MEDLINE]

Syrjala MT, Tiirikainen M, Jansson SE, Krusius T.

Flow cytometric analysis of terminal deoxynucleotidyl transferase. A simplified method.
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Feasibility of an easily applicable method of ZAP-70 measurement in chronic lymphocytic leukemia in the routine flow cytometry setting: A methodological approach.
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ZAP-70 by flow cytometry: a comparison of different antibodies, anticoagulants, and methods of analysis.
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Shenkin M, Maiese R.

Use of a blocking antibody method for the flow cytometric measurement of ZAP-70 in B-CLL.
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Shankey TV, Forman M, Scibelli P, Cobb J, Smith CM, Mills R, Holdaway K, Bernal-Hoyos E, Van Der Heiden M, Popma J, Keeney M.

An optimized whole blood method for flow cytometric measurement of ZAP-70 protein expression in chronic lymphocytic leukemia.
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Gibbs G, Bromidge T, Howe D, Hopkins J, Johnson S.

Comparison of flow cytometric methods for the measurement of ZAP-70 expression in a routine diagnostic laboratory.
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Chen YH, Peterson LC, Dittmann D, Evens A, Rosen S, Khoong A, Shankey TV, Forman M, Gupta R, Goolsby CL.

Comparative analysis of flow cytometric techniques in assessment of ZAP-70 expression in relation to IgVH mutational status in chronic lymphocytic leukemia.
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