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Gel Shift (Gel)
Protein-DNA/Protein-RNA Interactions
Funabashi H, Ubukata M, Ebihara T, Aizawa M, Mie M, Kobatake E.
Assessment of small ligand-protein interactions
by electrophoretic mobility shift assay using DNA-modified ligand as
a sensing probe.
Biotechnol Lett. 2007
May;29(5):785-9. Epub 2007 Feb 6.
PMID: 17279446 [PubMed - in process]
Stead JA, Keen JN, McDowall KJ.
The identification of nucleic acid-interacting
proteins using a simple proteomics-based approach that directly
incorporates the electrophoretic mobility shift assay.
Mol Cell Proteomics. 2006
Sep;5(9):1697-702. Epub 2006 Jul 15.
PMID: 16845145 [PubMed - indexed for MEDLINE]
Kang J, Lee MS, Watowich SJ, Gorenstein DG.
Chemiluminescence-based electrophoretic mobility
shift assay of RNA-protein interactions: application to binding of
viral capsid proteins to RNA.
J Virol Methods. 2006
Feb;131(2):155-9. Epub 2005 Sep 22.
PMID: 16182384 [PubMed - indexed for MEDLINE]
Wagner U, Wahle M, Malysheva O, Wagner U, Hantzschel H, Baerwald C.
Sequence variants of the CRH 5'-flanking region:
effects on DNA-protein interactions studied by EMSA in PC12 cells.
Ann N Y Acad Sci. 2006
Jun;1069:20-33.
PMID: 16855132 [PubMed - indexed for MEDLINE]
A modified quantitative EMSA and its application
in the study of RNA--protein interactions.
J Biochem Biophys Methods. 2004 Aug
31;60(2):85-96.
PMID: 15262445 [PubMed - indexed for MEDLINE]
Lyon M, Deakin JA, Lietha D, Gherardi E, Gallagher JT.
The interactions of hepatocyte growth
factor/scatter factor and its NK1 and NK2 variants with
glycosaminoglycans using a modified gel mobility shift assay.
Elucidation of the minimal size of binding and activatory
oligosaccharides.
J Biol Chem. 2004 Oct
15;279(42):43560-7. Epub 2004 Aug 2.
PMID: 15292253 [PubMed - indexed for MEDLINE]
Interactions of the 18.5 kDa isoform of myelin
basic protein with Ca2+-calmodulin: in vitro studies using gel shift
assays.
Mol Cell Biochem. 2002
Dec;241(1-2):45-52.
PMID: 12482024 [PubMed - indexed for MEDLINE]
Filee P, Delmarcelle M, Thamm I, Joris B.
Use of an ALFexpress DNA sequencer to analyze
protein-nucleic acid interactions by band shift assay.
Biotechniques. 2001 May;30(5):1044-8,
1050-1.
PMID: 11355340 [PubMed - indexed for MEDLINE]
Zemzoumi K, Dissous C, Cochu A, Trolet J, Capron A, McNair A.
Schistosoma mansoni: interaction of nuclear
extracts with the CCAAT-binding site revealed by the gel shift
assay.
Exp Parasitol. 1995 Feb;80(1):149-54.
PMID: 7821404 [PubMed - indexed for MEDLINE]
Ng L, McConnell M, Tan CK, Downey KM, Fisher PA.
Interaction of DNA polymerase delta,
proliferating cell nuclear antigen, and synthetic oligonucleotide
template-primers. Analysis by polyacrylamide gel
electrophoresis-band mobility shift assay.
J Biol Chem. 1993 Jun
25;268(18):13571-6.
PMID: 8099910 [PubMed - indexed for MEDLINE]
Engelender S, Giannini AL, Rumjanek FD.
Protein interactions with a gender-specific gene
of Schistosoma mansoni: characterization by DNase I footprinting,
band shift and UV cross-linking.
Mol Cell Biochem. 1993 Jul
21;124(2):159-68.
PMID: 7901756 [PubMed - indexed for MEDLINE]
Protein-DNA associations in a gender-specific
gene of Schistosoma mansoni: characterization by UV cross-linking,
DNase I footprinting and band shift assays.
Mem Inst Oswaldo Cruz. 1992;87 Suppl
4:67-70.
PMID: 1343927 [PubMed - indexed for MEDLINE]
Improved band shift assay for the simultaneous
analysis of protein-DNA interactions and enzymatic functions of DNA
polymerases.
FEBS Lett. 1992 Mar 30;300(2):141-4.
PMID: 1314195 [PubMed - indexed for MEDLINE]
Determination of binding constants for
cooperative site-specific protein-DNA interactions using the gel
mobility-shift assay.
J Biol Chem. 1991 Jul
25;266(21):13661-71.
PMID: 1856200 [PubMed - indexed for MEDLINE]
Measurement of protein-DNA interaction parameters
by electrophoresis mobility shift assay.
Electrophoresis. 1989
May-Jun;10(5-6):366-76.
PMID: 2670548 [PubMed - indexed for MEDLINE]