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Immunoblotting (IB)
Antigen-Antibody Specific Applications
ABH antibodies
Sato I, Hamabe T, Yamazaki K, Watanabe Y.
A new method for the determination of the ABO
blood type of semen by immunoblotting using anti-ABH antibodies
following immunoprecipitation.
J Immunol Methods. 1995 Dec
27;188(2):229-37.
PMID: 8551051 [PubMed - indexed for MEDLINE]
Allergens
An immunoblot technique for identification of
allergens of the Australian paralysis tick Ixodes holocyclus.
J Immunol Methods. 1990 Jan
24;126(1):51-5.
PMID: 2406343 [PubMed - indexed for MEDLINE]
Wijnands LM, Deisz WD, van Leusden FM.
Immunoblotting technique for the detection of
allergens of Aspergillus fumigatus: influence of Nonidet P-40 on the
sensitivity.
J Investig Allergol Clin Immunol.
1999 May-Jun;9(3):195-6.
PMID: 10412684 [PubMed - indexed for MEDLINE]
Gomez J, Mansfield LE, Frederick RW, Rael ED.
Analysis of the individual allergens of Russian
thistle pollen by an enzyme-linked immunoblotting technique.
J Asthma. 1989;26(4):243-50.
PMID: 2702232 [PubMed - indexed for MEDLINE]
Johansson E, van Hage-Hamsten M, Johansson SG.
Demonstration of allergen components in the
storage mite Lepidoglyphus destructor by an immunoblotting
technique.
Int Arch Allergy Appl Immunol.
1988;85(1):8-13.
PMID: 3338856 [PubMed - indexed for MEDLINE]
alpha 1B-glycoprotein
Gahne B, Juneja RK, Stratil A.
Genetic polymorphism of human plasma alpha
1B-glycoprotein: phenotyping by immunoblotting or by a simple method
of 2-D electrophoresis.
Hum Genet. 1987 Jun;76(2):111-5.
PMID: 3610142 [PubMed - indexed for MEDLINE]
Apolipoprotein
Genetic studies of human apolipoproteins. XV. An
overview of IEF immunoblotting methods to screen apolipoprotein
polymorphisms.
Hum Hered. 1990;40(4):193-207.
Review.
PMID: 2199367 [PubMed - indexed for MEDLINE]
Bence Jones protein
Heys AD, Norden AG, Fulcher LM, Flynn FV.
Bence Jones protein detection: a rapid
immunoblotting technique for routine use on unconcentrated urine.
Ann Clin Biochem. 1986 Sep;23 ( Pt
5):571-6.
PMID: 3767295 [PubMed - indexed for MEDLINE]
Chlamydia trachomatis and C. pneumoniae antigens
Gonen R, Shemer-Avni Y, Csango PA, Sarov B, Friedman MG.
Serum reactivity to Chlamydia trachomatis and C.
pneumoniae antigens in patients with documented infection and in
healthy children by microimmunofluorescence and immunoblotting
techniques.
APMIS. 1993 Sep;101(9):719-26.
PMID: 8240791 [PubMed - indexed for MEDLINE]
Deoxyribonuclease I
Yasuda T, Mizuta K, Ikehara Y, Kishi K.
Genetic analysis of human deoxyribonuclease I
by immunoblotting and the zymogram method following isoelectric
focusing.
Anal Biochem. 1989 Nov
15;183(1):84-8.
PMID: 2619050 [PubMed - indexed for MEDLINE]
Endothelin-1
Evans JJ, Youssef AH, Yandle TG, Lewis LK, Nicholls MG.
Endothelin-1 directly modulates its own
secretion: studies utilising the cell immunoblot technique.
Regul Pept. 2003 May
15;113(1-3):149-53.
PMID: 12686474 [PubMed - indexed for MEDLINE]
Entamoeba histolytica antigens
Berber AC, Escobar A, Zamora M, Acosta G.
Identification of Entamoeba histolytica antigens
recognized by IgA class human antibodies in sera and colostra of
puerperal women using immunoblotting techniques.
Arch Invest Med (Mex). 1990;21 Suppl
1:97-101.
PMID: 2136509 [PubMed - indexed for MEDLINE]
Enterovirus
Pozzetto B, Gaudin OG, Lucht FR, Hafid J, Ros A.
Detection of immunoglobulin G, M, and A
antibodies to enterovirus structural proteins by immunoblot
technique in echovirus type 4-infected patients.
J Virol Methods. 1990
Aug;29(2):143-55.
PMID: 2176661 [PubMed - indexed for MEDLINE]
Filarial antigens
Hussain R, Kaushal NA, Ottesen EA.
Comparison of immunoblot and
immunoprecipitation methods for analyzing cross-reactive antibodies
to filarial antigens.
J Immunol Methods. 1985 Nov
28;84(1-2):291-301.
PMID: 4067318 [PubMed - indexed for MEDLINE]
Glycophorins
Lu YQ, Nichols ME, Bigbee WL, Nagel RL, Blumenfeld OO.
Structural polymorphism of glycophorins
demonstrated by immunoblotting techniques.
Blood. 1987 Feb;69(2):618-24.
PMID: 3542081 [PubMed - indexed for MEDLINE]
Guanase
Ito S, Iwasaki A, Syundo J, Tamura Y, Kishi S, Mori H, Ii K, Matsuda
Y, Katsunuma N.
Characterization of antibody against human
liver guanase by immunoblotting and immunohistochemical staining of
human liver guanase by a direct labeling antibody-enzyme method.
J Histochem Cytochem. 1989
May;37(5):611-5.
PMID: 2649557 [PubMed - indexed for MEDLINE]
Helicobacter pylori
Immunoglobulin G antibodies to Helicobacter
pylori in patients with dyspeptic symptoms investigated by the
western immunoblot technique.
J Clin Microbiol. 1992
Jul;30(7):1743-51.
PMID: 1629330 [PubMed - indexed for MEDLINE]
Hemoglobins
Garver FA, Moscoso H, Kiefer CR.
Identification of abnormal hemoglobins in cord
blood by an immunoblot technique.
J Lab Clin Med. 1988
Feb;111(2):244-8.
PMID: 3339277 [PubMed - indexed for MEDLINE]
HSV-1/HSV-2
Immunoblotting and ELISA analysis of HSV-1 and
HSV-2-specified polypeptides by using an immunoblocking method.
Ann Clin Res. 1985;17(2):66-70.
PMID: 2412480 [PubMed - indexed for MEDLINE]
IgE/IgG/IgM/Igs
Xu HJ, Umapathysivam K, McNeilage J, Gordon TP, Roberts-Thomson PJ.
An enhanced chemiluminescence detection system
combined with a modified immunoblot technique for the detection of
low molecular weight IgM in sera from healthy adults and neonates.
J Immunol Methods. 1992 Feb
5;146(2):241-7.
PMID: 1538145 [PubMed - indexed for MEDLINE]
Specificity of IgM antibodies in acute human
coxsackievirus B infections, analysed by indirect solid phase enzyme
immunoassay and immunoblot technique.
J Gen Virol. 1983 Jan;64 (Pt
1):159-67.
PMID: 6185632 [PubMed - indexed for MEDLINE]
Beaume A, Brizard A, Dreyfus B, Preud'homme JL.
High incidence of serum monoclonal Igs detected
by a sensitive immunoblotting technique in B-cell chronic
lymphocytic leukemia.
Blood. 1994 Aug 15;84(4):1216-9.
PMID: 8049436 [PubMed - indexed for MEDLINE]
IgE-binding components in Pityrosporum orbiculare
identified by an immunoblotting technique.
Acta Derm Venereol. 1991;71(1):11-6.
PMID: 1676206 [PubMed - indexed for MEDLINE]
Trompelt J, Becker WM, Schlaak M.
Analysis of IgG subclass and IgE response in
allergic disease caused by Aspergillus fumigatus by immunoblotting
techniques.
Int Arch Allergy Immunol. 1994
Aug;104(4):390-8.
PMID: 8038618 [PubMed - indexed for MEDLINE]
Nooij FJ, Van der Sluijs-Gelling AJ, Jol-Van der Zijde CM, Van Tol
MJ, Haas H, Radl J.
Immunoblotting techniques for the detection of
low level homogeneous immunoglobulin components in serum.
J Immunol Methods. 1990 Dec
5;134(2):273-81.
PMID: 2254671 [PubMed - indexed for MEDLINE]
Lipoprotein
Measurement of small high density lipoprotein
subclass by an improved immunoblotting technique.
J Lipid Res. 1996 Nov;37(11):2461-9.
PMID: 8978497 [PubMed - indexed for MEDLINE]
Modified immunoblotting technique for phenotyping
lipoprotein(a).
Clin Chem. 1991 Apr;37(4):576-8.
PMID: 2015675 [PubMed - indexed for MEDLINE]
Luteinizing hormone-releasing hormone receptor
Eidne KA, Hendricks DT, Millar RP.
Demonstration of a 60K molecular weight
luteinizing hormone-releasing hormone receptor in solubilized
adrenal membranes by a ligand-immunoblotting technique.
Endocrinology. 1985
May;116(5):1792-5.
PMID: 2985361 [PubMed - indexed for MEDLINE]
Metallothioneins-1, -2 and -3
Mizzen CA, Cartel NJ, Yu WH, Fraser PE, McLachlan DR.
Sensitive detection of metallothioneins-1, -2
and -3 in tissue homogenates by immunoblotting: a method for
enhanced membrane transfer and retention.
J Biochem Biophys Methods. 1996 May
14;32(2):77-83.
PMID: 8796480 [PubMed - indexed for MEDLINE]
Mycobacterial antigens
Mathai A, Radhakrishnan VV, Sehgal S.
Characterization of mycobacterial antigens by
Elisa and immunoblot methods.
Indian J Pathol Microbiol. 1995
Jul;38(3):299-303.
PMID: 8819663 [PubMed - indexed for MEDLINE]
Myelin basic proteins (MBP)
Detection of Wolfgram W1 protein, myelin basic
proteins and proteolipids in cultured oligodendrocytes by the
electro-immunoblotting method.
Neurosci Lett. 1983 Sep
30;40(2):111-7.
PMID: 6195564 [PubMed - indexed for MEDLINE]
Oligoclonal IgG
Detection and identification of virus-specific,
oligoclonal IgG in unconcentrated cerebrospinal fluid by immunoblot
technique.
J Neuroimmunol. 1984
Dec;7(2-3):77-89.
PMID: 6511890 [PubMed - indexed for MEDLINE]
Ornithine decarboxylase
Pulkka A, Taskinen T, Aaltonen H, Ramberg J, Pajunen AE.
Studies on the degradation of ornithine
decarboxylase by the immunoblotting technique.
Biochem Int. 1985 Dec;11(6):845-51.
PMID: 4091857 [PubMed - indexed for MEDLINE]
Outer membrane proteins
Davies RL, Parton R, Coote JG, Gibbs HA, Freer JH.
Evaluation of different methods for the detection
of outer membrane proteins and lipopolysaccharides of Pasteurella
haemolytica by immunoblotting.
J Immunol Methods. 1994 Jan
3;167(1-2):35-45.
PMID: 7508480 [PubMed - indexed for MEDLINE]
Pepsinogen A
Zwiers A, Toonstra C, Pals G, Donker AJ, Meuwissen SG, ten Kate RW.
Immunoblot technique to visualise serum
pepsinogen A isozymogen patterns.
J Clin Pathol. 1990 Oct;43(10):871-4.
PMID: 2229438 [PubMed - indexed for MEDLINE]
Protein C inhibitor (PCI)
Yasuda T, Nadano D, Tanaka Y, Sawazaki K, Kishi K.
Novel detection method for human protein C
inhibitor (PCI) using immunoblotting with antibodies specific to
synthetic peptides corresponding to the N-terminal and C-terminal
amino acid sequences of human PCI.
Biochem Int. 1991 Oct;25(3):553-60.
PMID: 1666828 [PubMed - indexed for MEDLINE]
PSA
Wu JT, Zhang P, Wang T, Wilson L, Astill M.
Evaluation of free PSA isoforms, PSA complex
formation, and specificity of anti-PSA antibodies by HPLC and PAGE-immunoblotting
techniques.
J Clin Lab Anal. 1995;9(1):1-14.
PMID: 7536834 [PubMed - indexed for MEDLINE]
Rheumatoid factors
Identification of IgG rheumatoid factors by a
novel method utilizing immunoblotting.
J Immunol Methods. 1992 Apr
8;148(1-2):93-9.
PMID: 1564339 [PubMed - indexed for MEDLINE]
RNase 2
Mizuta K, Yasuda T, Ikehara Y, Sato W, Kishi K.
New detection method for ribonuclease 2 (RNase
2) using immunoblotting with specific antibody.
Z Rechtsmed. 1990;103(5):315-22.
PMID: 2356660 [PubMed - indexed for MEDLINE]
Rotavirus
Ushijima H, Shinozaki T, Kitamura T.
Detection of anti rotavirus coproantibodies by
immunoblotting technique.
J Med Virol. 1989 Oct;29(2):127-9.
PMID: 2557382 [PubMed - indexed for MEDLINE]
Thyroxin-binding globulin protein
A sensitive immunoblotting technique to identify
thyroxin-binding globulin protein heterogeneity after isoelectric
focusing.
Biochem Genet. 1986
Apr;24(3-4):273-80.
PMID: 3089215 [PubMed - indexed for MEDLINE]
Total protein S
Li X, Hatanaka K, Guo L, Tsushima M, Kitamura Y, Yamamoto A.
A sensitive peroxidase staining immunoblotting
method for measuring total protein S in human plasma.
Thromb Haemost. 1993 Apr
1;69(4):331-4.
PMID: 8497844 [PubMed - indexed for MEDLINE]
Toxoplasma gondii
Gross U, Bohne W, Schroder J, Roos T, Heesemann J.
Comparison of a commercial enzyme immunoassay
and an immunoblot technique for detection of immunoglobulin A
antibodies to Toxoplasma gondii.
Eur J Clin Microbiol Infect Dis. 1993
Aug;12(8):636-9.
PMID: 8223666 [PubMed - indexed for MEDLINE]
Partanen P, Turunen HJ, Paasivuo R, Forsblom E, Suni J, Leinikki PO.
Identification of antigenic components of
Toxoplasma gondii by an immunoblotting technique.
FEBS Lett. 1983 Jul 25;158(2):252-4.
PMID: 6873278 [PubMed - indexed for MEDLINE]
Tyrosine protein kinase
Evans JP, Wickremasinghe RG, Hoffbrand AV.
Detection of tyrosine protein kinase substrates
in fresh leukemia cells and normal blood cells using an
immunoblotting technique.
Leukemia. 1987 Nov;1(11):782-5.
PMID: 3316858 [PubMed - indexed for MEDLINE]
Evans JP, Wickremasinghe RG, Hoffbrand AV.
Tyrosine protein kinase substrates in
Philadelphia-positive human chronic granulocytic leukemia derived
cell lines (K562 and BV173): detection by using an immunoblotting
technique.
Leukemia. 1987 Jun;1(6):524-5.
PMID: 2444834 [PubMed - indexed for MEDLINE]
Urinary protein
Yamamoto T, Yoshimura S, Sasamori Y, Sakamoto T, Ogino M, Kambegawa
A, Okinaga S, Arai K.
Analysis of urinary protein by immunoblot
method using unconcentrated urine in preeclampsia.
Asia Oceania J Obstet Gynaecol. 1992
Jun;18(2):177-85.
PMID: 1380235 [PubMed - indexed for MEDLINE]
Uropepsinogen (PGA)
Yasuda T, Ikehara Y, Sato W, Kishi K.
New detection method for uropepsinogen (PGA)
using isoelectric focusing and immunoblotting techniques.
Z Rechtsmed. 1987;98(3):147-53.
PMID: 3307206 [PubMed - indexed for MEDLINE]