Immunocytochemistry (ICC)

General Methods & Techniques




St-Laurent J, Boulay ME, Prince P, Bissonnette E, Boulet LP.

Comparison of cell fixation methods of induced sputum specimens: an immunocytochemical analysis.
J Immunol Methods. 2006 Jan 20;308(1-2):36-42. Epub 2005 Nov 21.
PMID: 16325848 [PubMed - indexed for MEDLINE]


Paciorek T, Sauer M, Balla J, Wisniewska J, Friml J.

Immunocytochemical technique for protein localization in sections of plant tissues.
Nat Protoc. 2006;1(1):104-7.
PMID: 17406219 [PubMed - indexed for MEDLINE]


Agnati LF, Fuxe K, Torvinen M, Genedani S, Franco R, Watson S, Nussdorfer GG, Leo G, Guidolin D.

New methods to evaluate colocalization of fluorophores in immunocytochemical preparations as exemplified by a study on A2A and D2 receptors in Chinese hamster ovary cells.
J Histochem Cytochem. 2005 Aug;53(8):941-53.
PMID: 16055748 [PubMed - indexed for MEDLINE]


Lopez-Sanchez C, Garcia-Martinez V, Lawson A, Chapman SC, Schoenwolf GC.

Rapid triple-labeling method combining in situ hybridization and double immunocytochemistry.
Dev Dyn. 2004 Jun;230(2):309-15.
PMID: 15162509 [PubMed - indexed for MEDLINE]


Soini AE, Kuusisto A, Meltola NJ, Soini E, Seveus L.

A new technique for multiparameter imaging microscopy: use of long decay time photoluminescent labels enables multiple color immunocytochemistry with low channel-to-channel crosstalk.
Microsc Res Tech. 2003 Dec 1;62(5):396-407.
PMID: 14601145 [PubMed - indexed for MEDLINE]


Breschi L, Gobbi P, Lopes M, Prati C, Falconi M, Teti G, Mazzotti G.

Immunocytochemical analysis of dentin: a double-labeling technique.
J Biomed Mater Res A. 2003 Oct 1;67(1):11-7.
PMID: 14517856 [PubMed - indexed for MEDLINE]


Morris TJ, Stanley EF.

A simple method for immunocytochemical staining with multiple rabbit polyclonal antibodies.
J Neurosci Methods. 2003 Aug 15;127(2):149-55.
PMID: 12906944 [PubMed - indexed for MEDLINE]


Oertel J, Huhn D.

Immunocytochemical methods in haematology and oncology.
J Cancer Res Clin Oncol. 2000 Aug;126(8):425-40. Review.
PMID: 10961385 [PubMed - indexed for MEDLINE]


Burry RW.

Specificity controls for immunocytochemical methods.
J Histochem Cytochem. 2000 Feb;48(2):163-6.
PMID: 10639482 [PubMed - indexed for MEDLINE]


Wicht H, Maronde E, Olcese J, Korf HW.

A semiquantitative image-analytical method for the recording of dose-response curves in immunocytochemical preparations.
J Histochem Cytochem. 1999 Mar;47(3):411-20.
PMID: 10026243 [PubMed - indexed for MEDLINE]


Takizawa T, Saito T, Robinson JM.

Freeze-fracture cytochemistry: a new method combining immunocytochemistry and enzyme cytochemistry on replicas.
J Histochem Cytochem. 1998 Jan;46(1):11-7.
PMID: 10712099 [PubMed - indexed for MEDLINE]


Kawashima I, Tai T.

An immunocytochemical technique with monoclonal antibodies to glycosphingolipids in rat primary cerebellar cultures: influence of detergent permeabilization.
Brain Res Brain Res Protoc. 1998 Jun;2(4):299-305.
PMID: 9630687 [PubMed - indexed for MEDLINE]


Allgayer H, Heiss MM, Riesenberg R, Babic R, Jauch KW, Schildberg FW.

Immunocytochemical phenotyping of disseminated tumor cells in bone marrow by uPA receptor and CK18: investigation of sensitivity and specificity of an immunogold/alkaline phosphatase double staining protocol.
J Histochem Cytochem. 1997 Feb;45(2):203-12.
PMID: 9016310 [PubMed - indexed for MEDLINE]


Cloez-Tayarani I, Fillion G.

The in situ hybridization and immunocytochemistry techniques for characterization of cells expressing specific mRNAs in paraffin-embedded brains.
Brain Res Brain Res Protoc. 1997 May;1(2):195-202.
PMID: 9385084 [PubMed - indexed for MEDLINE]


Conley CA, Hanson MR.

Cryostat tissue printing: an improved method for histochemical and immunocytochemical localization in soft tissues.
Biotechniques. 1997 Mar;22(3):488, 491-6.
PMID: 9067027 [PubMed - indexed for MEDLINE]


Kuenen-Boumeester V, van Loenen P, de Bruijn EM, Henzen-Logmans SC.

Quality control of immunocytochemical staining of effusions using a standardized method of cell processing.
Acta Cytol. 1996 May-Jun;40(3):475-9.
PMID: 8669182 [PubMed - indexed for MEDLINE]


Mialhe A, Cassanelli S, Louis J, Seigneurin D.

Methods for simultaneous interphase in situ hybridization and nuclear antigen immunocytochemistry in T47-D cells.
J Histochem Cytochem. 1996 Feb;44(2):193-7.
PMID: 8609377 [PubMed - indexed for MEDLINE]


Hrabovszky E, Vrontakis ME, Petersen SL.

Triple-labeling method combining immunocytochemistry and in situ hybridization histochemistry: demonstration of overlap between Fos-immunoreactive and galanin mRNA-expressing subpopulations of luteinizing hormone-releasing hormone neurons in female rats.
J Histochem Cytochem. 1995 Apr;43(4):363-70.
PMID: 7534782 [PubMed - indexed for MEDLINE]


Garcia-Garcia A, Munoz-Cueto JA, Rodriguez RB, Sarasquete C.

Protein G-horseradish peroxidase based method for light-microscope immunocytochemistry. Application to the pituitary gland of the killifish, Fundulus heteroclitus.
Eur J Histochem. 1994;38(3):229-36.
PMID: 7530516 [PubMed - indexed for MEDLINE]


Burton SA, Dowsett M, Trott PA.

Comparison of two methods of storing breast fine needle aspirates (FNAs) using oestrogen receptor immunocytochemical assay as a method of evaluating the storage methods.
Cytopathology. 1994 Dec;5(6):380-3.
PMID: 7880971 [PubMed - indexed for MEDLINE]


Newkirk RF, Mack J.

Improved indirect fluorescence immunocytochemical method using counterstains.
Biotechniques. 1992 Oct;13(4):536-8.
PMID: 1282341 [PubMed - indexed for MEDLINE]


Davidoff MS, Schulze W, Holstein AF.

Combination of alkaline phosphatase anti-alkaline phosphatase (APAAP)- and avidin-biotin-alkaline phosphatase complex (ABAP)-techniques for amplification of immunocytochemical staining of human testicular tissue.
Andrologia. 1991 Sep-Oct;23(5):353-6.
PMID: 1724878 [PubMed - indexed for MEDLINE]


Gao KX, Godkin JD.

A new method for transfer of polyethylene glycol-embedded tissue sections to silanated slides for immunocytochemistry.
J Histochem Cytochem. 1991 Apr;39(4):537-40.
PMID: 2005376 [PubMed - indexed for MEDLINE]


Sugi Y, Hirakow R.

Cytoskeletal filaments in embryonic chick myocardial cells as revealed by the quick-freeze deep-etch method combined with immunocytochemistry.
Cell Tissue Res. 1991 Mar;263(3):459-69.
PMID: 1878933 [PubMed - indexed for MEDLINE]


Allen MH, Markey AC, MacDonald DM.

The development of a reproducible immunocytochemical technique for demonstrating colocalized cutaneous antigens.
Am J Dermatopathol. 1991 Jun;13(3):221-7.
PMID: 1867353 [PubMed - indexed for MEDLINE]


Barthel LK, Raymond PA.

Improved method for obtaining 3-microns cryosections for immunocytochemistry.
J Histochem Cytochem. 1990 Sep;38(9):1383-8.
PMID: 2201738 [PubMed - indexed for MEDLINE]


Davidoff M, Schulze W.

Combination of the peroxidase anti-peroxidase (PAP)- and avidin-biotin-peroxidase complex (ABC)-techniques: an amplification alternative in immunocytochemical staining.
Histochemistry. 1990;93(5):531-6.
PMID: 1692015 [PubMed - indexed for MEDLINE]


Weintraub J, Redard M, Wenger D, Vassilakos P.

The application of immunocytochemical techniques to routinely-fixed and stained cytologic specimens. An aid in the differential diagnosis of undifferentiated malignant neoplasms.
Pathol Res Pract. 1990 Oct;186(5):658-65.
PMID: 1705023 [PubMed - indexed for MEDLINE]


Berryman MA, Rodewald RD.

An enhanced method for post-embedding immunocytochemical staining which preserves cell membranes.
J Histochem Cytochem. 1990 Feb;38(2):159-70.
PMID: 1688894 [PubMed - indexed for MEDLINE]


Tashiro T, Ruda MA, Satoda T, Matsushima R, Mizuno N.

Convergence of serotonin-, enkephalin- and substance P-like immunoreactive afferent fibers onto cat medullary dorsal horn projection neurons: a triple immunocytochemical staining technique combined with the retrograde HRP-tracing method.
Brain Res. 1989 Jul 10;491(2):360-5.
PMID: 2475211 [PubMed - indexed for MEDLINE]


Musto P, Aieta M, Sperandeo G, Cammisa M, Carotenuto M.

Immunocytochemical typing of fine needle aspirates by means of alkaline phosphatase anti-alkaline phosphatase method. A study of 16 selected patients.
Ric Clin Lab. 1989 Apr-Jun;19(2):139-47.
PMID: 2672274 [PubMed - indexed for MEDLINE]


Tashiro T, Satoda T, Matsushima R, Mizuno N.

Convergence of serotonin-, enkephalin- and substance P-like immunoreactive afferent fibers on single pudendal motoneurons in Onuf's nucleus of the cat: a light microscope study combining the triple immunocytochemical staining technique with the retrograde HRP-tracing method.
Brain Res. 1989 Mar 6;481(2):392-8.
PMID: 2470468 [PubMed - indexed for MEDLINE]


Myers JD.

Development and application of immunocytochemical staining techniques: a review.
Diagn Cytopathol. 1989;5(3):318-30. Review.
PMID: 2477206 [PubMed - indexed for MEDLINE]


Dinges HP, Wirnsberger G, Hofler H.

Immunocytochemistry in cytology. Comparative evaluation of different techniques.
Anal Quant Cytol Histol. 1989 Feb;11(1):22-32.
PMID: 2655648 [PubMed - indexed for MEDLINE]


Stross WP, Jones M, Mason DY.

Automation of APAAP immunocytochemical technique.
J Clin Pathol. 1989 Jan;42(1):106-12.
PMID: 2646327 [PubMed - indexed for MEDLINE]


Mowery J, Chesner J, Spangenberger S, Hixson DC.

Rapid low molecular weight polyethylene glycol embedding protocol for immunocytochemistry.
J Histochem Cytochem. 1989 Oct;37(10):1549-52.
PMID: 2674274 [PubMed - indexed for MEDLINE]


Gloghini A, Cozzi M, Sulfaro S, Volpe R, Carbone A.

A combined cytochemical and immunocytochemical method for simultaneous visualization of cytoplasmic enzyme reactivity and cell surface antigens in cell suspensions.
Am J Clin Pathol. 1989 Jan;91(1):67-71.
PMID: 2462786 [PubMed - indexed for MEDLINE]


Correa FM, Guilhaume SS, Saavedra JM.

A simple two-step immunocytochemical method using protein A-peroxidase to stain immunoreactive cell antigens.
Brain Res. 1988 May 10;448(1):192-7.
PMID: 2898966 [PubMed - indexed for MEDLINE]


Gloghini A, Cozzi M, Sulfaro S, Volpe R, Carbone A.

Methods of simultaneous visualization of cytoplasmic enzyme reactivity and cell surface antigens (by cytochemistry combined with immunocytochemistry) in individual hematopoietic and lymphoid cells.
Int J Biol Markers. 1988 Oct-Dec;3(4):221-32. Review.
PMID: 3069937 [PubMed - indexed for MEDLINE]


Thomas N, Bennett R, Jones CN.

A comparison of immunocytochemical staining enhancement methods using a rapid microtitre immunocytochemistry assay (MIA).
J Immunol Methods. 1987 Nov 23;104(1-2):201-7.
PMID: 3316394 [PubMed - indexed for MEDLINE]


van der Heijden F.

An immunocytochemical double staining technique applied to intraepithelial lymphocytes in the small intestine of the rat.
Acta Histochem. 1987;82(1):59-62.
PMID: 3122507 [PubMed - indexed for MEDLINE]


Maddox PH, Tay SK, Jenkins D.

A new fixed cryosection technique for the simultaneous immunocytochemical demonstration of T6 and S100 antigens.
Histochem J. 1987 Jan;19(1):35-8.
PMID: 3108204 [PubMed - indexed for MEDLINE]


Swanson PE, Hagen KA, Wick MR.

Avidin-biotin-peroxidase-antiperoxidase (ABPAP) complex. An immunocytochemical method with enhanced sensitivity.
Am J Clin Pathol. 1987 Aug;88(2):162-76.
PMID: 2441594 [PubMed - indexed for MEDLINE]


Thomas N, Bennett R, Jones CN.

A comparison of immunocytochemical staining enhancement methods using a rapid microtitre immunocytochemistry assay (MIA).
J Immunol Methods. 1987 Nov 23;104(1-2):201-7.
PMID: 3316394 [PubMed - indexed for MEDLINE]


Matthews JB.

Immunocytochemical methods: a technical overview.
J Oral Pathol. 1987 Apr;16(4):189-95. Review.
PMID: 2442334 [PubMed - indexed for MEDLINE]


Ottersen OP, Storm-Mathisen J, Madsen S, Skumlien S, Stromhaug J.

Evaluation of the immunocytochemical method for amino acids.
Med Biol. 1986;64(2-3):147-58. Review.
PMID: 2875230 [PubMed - indexed for MEDLINE]


Liposits Z, Sherman D, Phelix C, Paull WK.

A combined light and electron microscopic immunocytochemical method for the simultaneous localization of multiple tissue antigens. Tyrosine hydroxylase immunoreactive innervation of corticotropin releasing factor synthesizing neurons in the paraventricular nucleus of the rat.
Histochemistry. 1986;85(2):95-106.
PMID: 2875047 [PubMed - indexed for MEDLINE]


Scopsi L, Bock E, Larsson LI.

Monoclonal antibody immunocytochemistry: novel method extending usefulness of monoclonal antibodies for antigen visualization.
Eur J Cell Biol. 1986 Jun;41(1):97-101.
PMID: 3539604 [PubMed - indexed for MEDLINE]


Scopsi L, Larsson LI.

Increased sensitivity in peroxidase immunocytochemistry. A comparative study of a number of peroxidase visualization methods employing a model system.
Histochemistry. 1986;84(3):221-30.
PMID: 3519544 [PubMed - indexed for MEDLINE]


Bendayan M, Duhr MA.

Modification of the protein A-gold immunocytochemical technique for the enhancement of its efficiency.
J Histochem Cytochem. 1986 May;34(5):569-75.
PMID: 2422247 [PubMed - indexed for MEDLINE]


Frickhofen N, Bross KJ, Heit W, Heimpel H.

Modified immunocytochemical slide technique for demonstrating surface antigens on viable cells.
J Clin Pathol. 1985 Jun;38(6):671-6.
PMID: 3891791 [PubMed - indexed for MEDLINE]


Childs GV, Unabia G, Tibolt R.

How the fixation-embedding protocol affects the specificity and efficiency of immunocytochemical stains for gonadotropin subunits.
Am J Anat. 1985 Dec;174(4):409-17.
PMID: 2417475 [PubMed - indexed for MEDLINE]


Scopsi L, Larsson LI.

Increased sensitivity in immunocytochemistry. Effects of double application of antibodies and of silver intensification on immunogold and peroxidase-antiperoxidase staining techniques.
Histochemistry. 1985;82(4):321-9.
PMID: 2409059 [PubMed - indexed for MEDLINE]


Hopwood D, Coghill G, Ramsay J, Milne G, Kerr M.

Microwave fixation: its potential for routine techniques, histochemistry, immunocytochemistry and electron microscopy.
Histochem J. 1984 Nov;16(11):1171-91.
PMID: 6210272 [PubMed - indexed for MEDLINE]


Pool CW, Madlener S, Diegenbach PC, Sluiter AA, van der Sluis P.

Quantification of antiserum reactivity in immunocytochemistry. Two new methods for measuring peroxidase activity on antigen-coupled beads incubated according to an immunocytoperoxidase method.
J Histochem Cytochem. 1984 Sep;32(9):921-8.
PMID: 6205048 [PubMed - indexed for MEDLINE]


Smithson KG, MacVicar BA, Hatton GI.

Polyethylene glycol embedding: a technique compatible with immunocytochemistry, enzyme histochemistry, histofluorescence and intracellular staining.
J Neurosci Methods. 1983 Jan;7(1):27-41.
PMID: 6188002 [PubMed - indexed for MEDLINE]


Bendayan M.

Double immunocytochemical labeling applying the protein A-gold technique.
J Histochem Cytochem. 1982 Jan;30(1):81-5.
PMID: 6172469 [PubMed - indexed for MEDLINE]


Baskin DG, Erlandsen SL, Parsons JA.

Immunocytochemistry with osmium-fixed tissue. I. Light microscopic localization of growth hormone and prolactin with the unlabeled antibody-enzyme method.
J Histochem Cytochem. 1979 Apr;27(4):867-72.
PMID: 109497 [PubMed - indexed for MEDLINE]


Wachsmuth ED.

Assessment of immunocytochemical techniques with particular reference to the mixed-aggregation immunocytochemical technique.
Ciba Found Symp. 1979;(73):135-59.
PMID: 95185 [PubMed - indexed for MEDLINE]


Tramu G, Pillez A, Leonardelli J.

An efficient method of antibody elution for the successive or simultaneous localization of two antigens by immunocytochemistry.
J stochem Cytochem. 1978 Apr;26(4):322-4.
PMPMID: 207771 [PubMed - indexed for MEDLINE]