Immunocytochemistry (ICC)

Tumor, Disease & Diagnostic Applications




General Methods

Pettigrew NM.

Techniques in immunocytochemistry. Application to diagnostic pathology.
Arch Pathol Lab Med. 1989 Jun;113(6):641-4. Review.
PMID: 2658908 [PubMed - indexed for MEDLINE]

Wazir JF, Martin-Bates E, Woodward G, Coleman DV.

Evaluation of immunocytochemical staining as a method of improving diagnostic accuracy in a routine cytopathology laboratory.
Cytopathology. 1991;2(2):75-82.
PMID: 1717024 [PubMed - indexed for MEDLINE]


Alzheimer's Disease

Velasco ME.

The copper-peroxide-silver method: a highly sensitive procedure for the demonstration of Alzheimer's disease lesions and for signal intensification in immunocytochemistry.
Mod Pathol. 1992 Jul;5(4):455-63.
PMID: 1495951 [PubMed - indexed for MEDLINE]

Davies L, Wolska B, Hilbich C, Multhaup G, Martins R, Simms G, Beyreuther K, Masters CL.

A4 amyloid protein deposition and the diagnosis of Alzheimer's disease: prevalence in aged brains determined by immunocytochemistry compared with conventional neuropathologic techniques.
Neurology. 1988 Nov;38(11):1688-93.
PMID: 3054625 [PubMed - indexed for MEDLINE]


Anaplastic Thyroid Tumours

Ralfkiaer N, Gatter KC, Alcock C, Heryet A, Ralfkiaer E, Mason DY.

The value of immunocytochemical methods in the differential diagnosis of anaplastic thyroid tumours.
Br J Cancer. 1985 Aug;52(2):167-70.
PMID: 3896289 [PubMed - indexed for MEDLINE]


Brain Tumors

Morimura T, Kitz K, Budka H.

In situ analysis of cell kinetics in human brain tumors. A comparative immunocytochemical study of S phase cells by a new in vitro bromodeoxyuridine-labeling technique, and of proliferating pool cells by monoclonal antibody Ki-67.
Acta Neuropathol (Berl). 1989;77(3):276-82.
PMID: 2922990 [PubMed - indexed for MEDLINE]


Breast Cancer

Berois N, Varangot M, Osinaga E, Babino A, Caignault L, Muse I, Roseto A.

Detection of rare human breast cancer cells. Comparison of an immunomagnetic separation method with immunocytochemistry and RT-PCR.
Anticancer Res. 1997 Jul-Aug;17(4A):2639-46.
PMID: 9252694 [PubMed - indexed for MEDLINE]

Lukashina MI, Zhukova LG, Glukhova EI, Ozerova OA, Ermilova VD, Bogatyrev VN, Baryshnikov AIu.

Comparison of immunocytochemical and immunohistochemical methods for biomarker detection in breast cancer
Klin Lab Diagn. 2002 Aug;(8):37-41. Russian.
PMID: 12362640 [PubMed - indexed for MEDLINE]

De Negri F, Campani D, Sarnelli R, Martini L, Gigliotti A, Bonacci R, Fabbri R, Squartini F, Pinchera A, Giani C.

Comparison of monoclonal immunocytochemical and immunoenzymatic methods for steroid receptor evaluation in breast cancer.
Am J Clin Pathol. 1991 Jul;96(1):53-8.
PMID: 2069136 [PubMed - indexed for MEDLINE]

Bozzetti C, Nizzoli R, Naldi N, Manotti L, Savoldi L, Camisa R, Guazzi A, Cocconi G.

Fine-needle aspiration technique for the concurrent immunocytochemical evaluation of multiple biologic parameters in primary breast carcinoma.
Breast Cancer Res Treat. 1994;32(2):221-8.
PMID: 7865851 [PubMed - indexed for MEDLINE]

Amoils KD, Seymour L, Bezwoda WR.

Comparison between immunocytochemical and polymerase chain reaction techniques for detection of oestrogen receptor and transforming growth factor beta in breast cancer.
Br J Cancer. 1996 May;73(10):1255-9.
PMID: 8630288 [PubMed - indexed for MEDLINE]

Wand H, Kunde D, Vogt R, Heise E.

Studies on assay of estradiol binding proteins by cytochemical and immunocytochemical techniques in human breast tumors and other tissues.
Arch Geschwulstforsch. 1985;55(6):467-72.
PMID: 3936452 [PubMed - indexed for MEDLINE]



Yang Y, Schmitt HP.

Frontotemporal dementia: evidence for impairment of ascending serotoninergic but not noradrenergic innervation. Immunocytochemical and quantitative study using a graph method.
Acta Neuropathol (Berl). 2001 Mar;101(3):256-70.
PMID: 11307626 [PubMed - indexed for MEDLINE]

Yang Y, Beyreuther K, Schmitt HP.

Spatial analysis of the neuronal density of aminergic brainstem nuclei in primary neurodegenerative and vascular dementia: a comparative immunocytochemical and quantitative study using a graph method.
Anal Cell Pathol. 1999;19(3-4):125-38.
PMID: 10866275 [PubMed - indexed for MEDLINE]


Gastrointestinal Stromal Tumor

Lozano MD, Rodriguez J, Algarra SM, Panizo A, Sola JJ, Pardo J.

Fine-needle aspiration cytology and immunocytochemistry in the diagnosis of 24 gastrointestinal stromal tumors: a quick, reliable diagnostic method.
Diagn Cytopathol. 2003 Mar;28(3):131-5.
PMID: 12619093 [PubMed - indexed for MEDLINE]


Germ Cell Tumors

Hildebrandt MO, Blaser F, Beyer J, Siegert W, Mapara MY, Huhn D, Salama A.

Detection of tumor cells in peripheral blood samples from patients with germ cell tumors using immunocytochemical and reverse transcriptase-polymerase chain reaction techniques.
Bone Marrow Transplant. 1998 Oct;22(8):771-5.
PMID: 9827974 [PubMed - indexed for MEDLINE]


Head and Neck Neoplasms

Abemayor E, Kessler DJ, Ward PH, Fu YS.

Evaluation of poorly differentiated head and neck neoplasms. Immunocytochemistry techniques.
Arch Otolaryngol Head Neck Surg. 1987 May;113(5):506-9.
PMID: 3551997 [PubMed - indexed for MEDLINE]


Helicobacter pylori

Faraker CA.

Diagnosis of Helicobacter pylori in gastric brush and biopsy specimens stained by Romanowsky and immunocytochemical methods: comparison with the CLOtest.
Cytopathology. 1996 Apr;7(2):108-19.
PMID: 9074660 [PubMed - indexed for MEDLINE]


Hematologic Neoplasms

Diaz de Domingo NB, Lardo MM, Romero Artaza J, Merelli A, Carbia CD, Sanchez Avalos JC.

Immunocytochemistry techniques for the diagnosis of hematologic neoplasms
Medicina (B Aires). 1999;59(1):11-6. Spanish.
PMID: 10349112 [PubMed - indexed for MEDLINE]



Kass L, Elias JM.

Cytochemistry and immunocytochemistry in bone marrow examination: contemporary techniques for the diagnosis of acute leukemia and myelodysplastic syndromes. A combined approach.
Hematol Oncol Clin North Am. 1988 Dec;2(4):537-55. Review.
PMID: 3065317 [PubMed - indexed for MEDLINE]

Ho AD, Helmstadter V, Hunstein W.

Immunocytochemical method for the detection of terminal deoxynucleotidyl transferase in acute leukemia.
Klin Wochenschr. 1982 May 3;60(9):451-5. Review.
PMID: 7045509 [PubMed - indexed for MEDLINE]


Lung Cancer

Scott F, Cuttitta F, Treston AM, Avis I, Gupta P, Ruckdeschel J, Kelly K, Piantadosi S, Tockman M, Mulshine J.

Prospective trial evaluating immunocytochemical-based sputum techniques for early lung cancer detection: assays for promotion factors in the bronchial lavage.
J Cell Biochem Suppl. 1993;17F:175-83.
PMID: 8412189 [PubMed - indexed for MEDLINE]



Brandtzaeg P, Rognum TO.

Evaluation of tissue preparation methods and paired immunofluorescence staining for immunocytochemistry of lymphomas.
Histochem J. 1983 Jul;15(7):655-89.
PMID: 6350234 [PubMed - indexed for MEDLINE]

Taylor CR.

Immunocytochemical methods in the study of lymphoma and related conditions.
J Histochem Cytochem. 1978 Jul;26(7):496-512.
PMID: 357639 [PubMed - indexed for MEDLINE]

Simsir A, Fetsch P, Stetler-Stevenson M, Abati A.

Immunophenotypic analysis of non-Hodgkin's lymphomas in cytologic specimens: a correlative study of immunocytochemical and flow cytometric techniques.
Diagn Cytopathol. 1999 May;20(5):278-84.
PMID: 10319228 [PubMed - indexed for MEDLINE]


Lymphoproliferative diseases

Franssila KO, Lindholm C, Teerenhovi L, Knuutila S.

A method combining morphological, immunocytochemical and chromosomal examinations of the same cell in the study of lymphoproliferative diseases.
Eur J Haematol. 1988 Apr;40(4):332-8.
PMID: 3366224 [PubMed - indexed for MEDLINE]


Malignant Effusions

el-Habashi A, el-Morsi B, Freeman SM, el-Didi M, Marrogi AJ.

Tumor oncogenic expression in malignant effusions as a possible method to enhance cytologic diagnostic sensitivity. An immunocytochemical study of 87 cases.
Am J Clin Pathol. 1995 Feb;103(2):206-14.
PMID: 7856564 [PubMed - indexed for MEDLINE]



Hoinghaus R, Mischke R, Hewicker-Trautwein M.

Use of immunocytochemical techniques in canine melanoma.
J Vet Med A Physiol Pathol Clin Med. 2002 May;49(4):198-202.
PMID: 12069262 [PubMed - indexed for MEDLINE]



Sloane JP, Ormerod MG, Neville AM.

Potential pathological application of immunocytochemical methods to the detection of micrometastases.
Cancer Res. 1980 Aug;40(8 Pt 2):3079-82.
PMID: 7397703 [PubMed - indexed for MEDLINE]


Neoplastic Effusions

Linari A, Bussolati G.

Evaluation of impact of immunocytochemical techniques in cytological diagnosis of neoplastic effusions.
J Clin Pathol. 1989 Nov;42(11):1184-9.
PMID: 2685053 [PubMed - indexed for MEDLINE]


Pancreatic Cancer

Nomoto S, Nakao A, Kasai Y, Inoue S, Harada A, Nonami T, Takagi H.

Peritoneal washing cytology combined with immunocytochemical staining and detecting mutant K-ras in pancreatic cancer: comparison of the sensitivity and availability of various methods.
Pancreas. 1997 Mar;14(2):126-32.
PMID: 9057184 [PubMed - indexed for MEDLINE]


Prostatic Carcinoma

Mansi JL, Berger U, Wilson P, Shearer R, Coombes RC.

Detection of tumor cells in bone marrow of patients with prostatic carcinoma by immunocytochemical techniques.
J Urol. 1988 Mar;139(3):545-8.
PMID: 2449548 [PubMed - indexed for MEDLINE]


Pulmonary Aspiration

Elidemir O, Fan LL, Colasurdo GN.

A novel diagnostic method for pulmonary aspiration in a murine model. Immunocytochemical staining of milk proteins in alveolar macrophages.
Am J Respir Crit Care Med. 2000 Feb;161(2 Pt 1):622-6.
PMID: 10714979 [PubMed - indexed for MEDLINE]


Soft Tissue Tumors

Nga ME, Lim GL, Barbro N, Chan NH.

Successful retrieval of fine-needle aspiration biopsy material from previously stained smears for immunocytochemistry: a novel technique applied to three soft tissue tumors.
Mod Pathol. 2005 May;18(5):728-32.
PMID: 15578068 [PubMed - indexed for MEDLINE]


Solid Tumors

Henn W, Lehr J, Unteregger G, Ermis A, Zang KD.

In situ chromosome preparation technique for simultaneous cytogenetic and immunocytochemical studies on cell cultures of solid tumors.
Hum Genet. 1990 Oct;85(5):551-4.
PMID: 2227941 [PubMed - indexed for MEDLINE]


Tuberculous Meningitis

Sumi MG, Mathai A, Reuben S, Sarada C, Radhakrishnan VV.

Immunocytochemical method for early laboratory diagnosis of tuberculous meningitis.
Clin Diagn Lab Immunol. 2002 Mar;9(2):344-7.
PMID: 11874875 [PubMed - indexed for MEDLINE]



Herbener GH.

Use of the protein A-gold immunocytochemical and enzyme-gold cytochemical techniques in studies of vitellogenesis.
Am J Anat. 1989 Jun-Jul;185(2-3):244-54.
PMID: 2788986 [PubMed - indexed for MEDLINE]