Immunoprecipitation (IP)

Antigen-Antibody Specific Applications

 

 

 

5C3

Larkin A, Moran E, Kennedy SM, Clynes M.

Monoclonal antibody 5C3 raised against formalin fixed paraffin-embedded invasive breast tumour tissue: characterisation of its reactive antigen via immunoprecipitation and internal sequencing.
J Immunol Methods. 2005 Aug;303(1-2):53-65.
PMID: 16038928 [PubMed - indexed for MEDLINE]

 

170-kDa bullous pemphigoid antigen

Ebihara T, Hashimoto T, Kudoh J, Gamou S, Shimizu N, Nishikawa T.

Detection of the 170-kDa bullous pemphigoid antigen by immunoprecipitation.
J Invest Dermatol. 1993 Feb;100(2):176-9.
PMID: 8429239 [PubMed - indexed for MEDLINE]

 

A1 adenosine receptor-GTP-binding protein

Wax MB, Patil RV.

Immunoprecipitation of A1 adenosine receptor-GTP-binding protein complexes in ciliary epithelial cells.
Invest Ophthalmol Vis Sci. 1994 Jun;35(7):3057-63.
PMID: 8206723 [PubMed - indexed for MEDLINE]

 

AA4

Stephan V, Guo N, Ginsburg V, Siraganian RP.

Immunoprecipitation of membrane proteins from rat basophilic leukemia cells by the antiganglioside monoclonal antibody AA4.
J Immunol. 1991 Jun 15;146(12):4271-7.
PMID: 1828263 [PubMed - indexed for MEDLINE]

 

ABH

Sato I, Hamabe T, Yamazaki K, Watanabe Y.

A new method for the determination of the ABO blood type of semen by immunoblotting using anti-ABH antibodies following immunoprecipitation.
J Immunol Methods. 1995 Dec 27;188(2):229-37.
PMID: 8551051 [PubMed - indexed for MEDLINE]

 

Albumin

Algranati ID.

Improved method for the specific immunoprecipitation of albumin.
Mol Cell Biochem. 1979 Oct 15;27(2):85-8.
PMID: 388187 [PubMed - indexed for MEDLINE]

 

Amyloid beta

Clarke NJ, Tomlinson AJ, Ohyagi Y, Younkin S, Naylor S.

Detection and quantitation of cellularly derived amyloid beta peptides by immunoprecipitation-HPLC-MS.
FEBS Lett. 1998 Jul 3;430(3):419-23.
PMID: 9688584 [PubMed - indexed for MEDLINE]

 

Adrenocortical actin

Cheitlin RA, Ramachandran J.

Purification of rat adrenocortical actin and its use in an immunoprecipitation assay to quantitate cellular actin.
Biochim Biophys Acta. 1986 Sep 4;883(2):383-7.
PMID: 3527278 [PubMed - indexed for MEDLINE]

 

Adrenal microsomal antigen

Furmaniak J, Talbot D, Reinwein D, Benker G, Creagh FM, Rees Smith B.

Immunoprecipitation of human adrenal microsomal antigen.
FEBS Lett. 1988 Apr 11;231(1):25-8.
PMID: 3360129 [PubMed - indexed for MEDLINE]

 

beta-amyloid peptide

Portelius E, Westman-Brinkmalm A, Zetterberg H, Blennow K.

Determination of beta-amyloid peptide signatures in cerebrospinal fluid using immunoprecipitation-mass spectrometry.
J Proteome Res. 2006 Apr;5(4):1010-6.
PMID: 16602710 [PubMed - indexed for MEDLINE]

 

c-myb

Maly A, Krchnak V.

Identification of c-myb (chicken), c-myb (mouse) and v-myb (AMV) protein products by immunoprecipitation with antibodies directed against a synthetic peptide.
FEBS Lett. 1986 Sep 1;205(1):104-8.
PMID: 3527747 [PubMed - indexed for MEDLINE]

 

Erythrocyte antigen

Laing P, Parkar BA, Culbert EJ, Watt GJ, Elson CJ.

Identification of rat erythrocyte antigens with a new non-radioactive immunoprecipitation technique.
Scand J Immunol. 1987 Jun;25(6):613-20.
PMID: 3602935 [PubMed - indexed for MEDLINE]

 

Erythropoietin

Widness JA, Schmidt RL, Veng-Pedersen P, Modi NB, Sawyer ST.

A sensitive and specific erythropoietin immunoprecipitation assay: application to pharmacokinetic studies.
J Lab Clin Med. 1992 Mar;119(3):285-94.
PMID: 1311741 [PubMed - indexed for MEDLINE]

 

Factor VIII

Klinge J, Auerswald G, Budde U, Klose H, Kreuz W, Lenk H, Scandella D; Paediatric Inhibitor Study Group of the German Society on Thrombosis and Haemostasis.

Detection of all anti-factor VIII antibodies in haemophilia A patients by the Bethesda assay and a more sensitive immunoprecipitation assay.
Haemophilia. 2001 Jan;7(1):26-32.
PMID: 11136377 [PubMed - indexed for MEDLINE]

 

Fonsecaea pedrosoi

Vidal MS, de Castro LG, Cavalecate SC, Lacaz Cda S.

Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis.
Rev Inst Med Trop Sao Paulo. 2003 Nov-Dec;45(6):315-8. Epub 2004 Jan 26.
PMID: 14762630 [PubMed - indexed for MEDLINE]

 

GAD65

Akamine H, Komiya I, Shimabukuro T, Asawa T, Tanaka H, Yagi N, Taira T, Nagata K, Arakaki K, Wakugami T, Takasu N, Powell MJ, Furmaniak J, Smith BR.

High prevalence of GAD65 (and IA-2) antibodies in Japanese IDDM patients by a new immunoprecipitation assay based on recombinant human GAD65.
Diabet Med. 1997 Sep;14(9):778-84.
PMID: 9300229 [PubMed - indexed for MEDLINE]

 

gamma-aminobutyric acid A receptor

Duggan MJ, Pollard S, Stephenson FA.

Quantitative immunoprecipitation studies with anti-gamma-aminobutyric acidA receptor gamma 2 1-15 Cys antibodies.
J Neurochem. 1992 Jan;58(1):72-7.
PMID: 1309240 [PubMed - indexed for MEDLINE]

 

Growth hormone binding protein

Ho KK, Valiontis E, Waters MJ, Rajkovic IA.

Regulation of growth hormone binding protein in man: comparison of gel chromatography and immunoprecipitation methods.
J Clin Endocrinol Metab. 1993 Feb;76(2):302-8.
PMID: 8432772 [PubMed - indexed for MEDLINE]

 

HSP101

Zhang C, Guy CL.

Co-immunoprecipitation of Hsp101 with cytosolic Hsc70.
Plant Physiol Biochem. 2005 Jan;43(1):13-8. Epub 2005 Jan 21.
PMID: 15763661 [PubMed - indexed for MEDLINE]

 

Human platelet alloantigens and glycoproteins

Smith JW, Hayward CP, Warkentin TE, Horsewood P, Kelton JG.

Investigation of human platelet alloantigens and glycoproteins using non-radioactive immunoprecipitation.
J Immunol Methods. 1993 Jan 14;158(1):77-85.
PMID: 8429218 [PubMed - indexed for MEDLINE]

 

IA-2

Masuda M, Powell M, Chen S, Beer C, Fichna P, Rees Smith B, Furmaniak J.

Autoantibodies to IA-2 in insulin-dependent diabetes mellitus. Measurements with a new immunoprecipitation assay.
Clin Chim Acta. 2000 Jan 20;291(1):53-66.
PMID: 10612717 [PubMed - indexed for MEDLINE]

 

IgE

Kolmannskog S, Haneberg B, Florholmen J.

Detection of immunoglobulin E in feces by immunoprecipitation, and characterization of associated non-immunoglobulin precipitins.
Int Arch Allergy Appl Immunol. 1985;76(3):226-31.
PMID: 3918941 [PubMed - indexed for MEDLINE]

 

IgG

Michaelsen TE, Haug E.

Human IgG subclass-specific rabbit antisera suitable for immunoprecipitation in gel, ELISA and multilayer haemagglutination techniques.
J Immunol Methods. 1985 Nov 28;84(1-2):203-20.
PMID: 2415635 [PubMed - indexed for MEDLINE]

 

Insulin receptor

Bailyes EM, Nave BT, Soos MA, Orr SR, Hayward AC, Siddle K.

Insulin receptor/IGF-I receptor hybrids are widely distributed in mammalian tissues: quantification of individual receptor species by selective immunoprecipitation and immunoblotting.
Biochem J. 1997 Oct 1;327 ( Pt 1):209-15.
PMID: 9355755 [PubMed - indexed for MEDLINE]

Perrotti N, Taylor SI, Richert ND, Rapp UR, Pastan IH, Roth J.

Immunoprecipitation of insulin receptors from cultured human lymphocytes (IM-9 cells) by antibodies to pp60src.
Science. 1985 Feb 15;227(4688):761-3.
PMID: 3918346 [PubMed - indexed for MEDLINE]

Chvatchko Y, Van Obberghen E, Kiger N, Fehlmann M.

Immunoprecipitation of insulin receptors by antibodies against Class 1 antigens of the murine H-2 major histocompatibility complex.
FEBS Lett. 1983 Nov 14;163(2):207-11.
PMID: 6196229 [PubMed - indexed for MEDLINE]

 

Interferon (IFN)-beta

Lampasona V, Rio J, Franciotta D, Furlan R, Avolio C, Fazio R, Lavolpe V, Vincent A, Comi G, Trojano M, Montalban X, Martino G.

Serial immunoprecipitation assays for interferon--(IFN)-beta antibodies in multiple sclerosis patients.
Eur Cytokine Netw. 2003 Jul-Sep;14(3):154-7.
PMID: 14656689 [PubMed - indexed for MEDLINE]

 

Interleukin-1 receptor

Rangnekar VM, Thomas LS, Plate JM.

Immunoprecipitation of interleukin-1 receptors from a mouse thymoma cell line, EL-4.
J Biol Chem. 1988 Nov 5;263(31):16408-13.
PMID: 2972709 [PubMed - indexed for MEDLINE]

 

Interleukin-2 receptor

Schwarting R, Gerdes J, Ziegler A, Stein H.

Immunoprecipitation of the interleukin-2 receptor from Hodgkin's disease derived cell lines by monoclonal antibodies.
Hematol Oncol. 1987 Jan-Mar;5(1):57-64.
PMID: 3106185 [PubMed - indexed for MEDLINE]

 

Intestinal alkaline phosphatase

Lehmann FG, Cramer P, Hillert U.

Intestinal alkaline phosphatase: an immunoprecipitation method for the determination in feces.
Clin Chim Acta. 1980 Aug 19;105(3):367-76.
PMID: 6996870 [PubMed - indexed for MEDLINE]

 

Lactose transporter

Seckler R.

Quantitative immunoprecipitation of the lactose transporter from Escherichia coli confirms stoichiometry of substrate binding.
Biochem Biophys Res Commun. 1986 Jan 29;134(2):975-81.
PMID: 3511912 [PubMed - indexed for MEDLINE]

 

Lipoprotein lipase

Doolittle MH, Ben-Zeev O, Briquet-Laugier V.

Enhanced detection of lipoprotein lipase by combining immunoprecipitation with Western blot analysis.
J Lipid Res. 1998 Apr;39(4):934-42.
PMID: 9555956 [PubMed - indexed for MEDLINE]

 

Lutropin receptor

Metsikko MK, Rajaniemi HJ.

Immunoprecipitation of the lutropin receptor. Loss of receptor molecules during down-regulation.
Biochem J. 1984 Dec 1;224(2):467-71.
PMID: 6097219 [PubMed - indexed for MEDLINE]

 

Malarial acid endopeptidase

Hempelmann E, Putfarken B, Rangachari K, Wilson RJ.

Immunoprecipitation of malarial acid endopeptidase.
Parasitology. 1986 Apr;92 ( Pt 2):305-12.
PMID: 3520446 [PubMed - indexed for MEDLINE]

 

Melanocytes

Naughton GK, Eisinger M, Bystryn JC.

Detection of antibodies to melanocytes in vitiligo by specific immunoprecipitation.
J Invest Dermatol. 1983 Dec;81(6):540-2.
PMID: 6196421 [PubMed - indexed for MEDLINE]

 

Muscle-specific receptor tyrosine kinase autoantibodies

Matthews I, Chen S, Hewer R, McGrath V, Furmaniak J, Rees Smith B.

Muscle-specific receptor tyrosine kinase autoantibodies--a new immunoprecipitation assay.
Clin Chim Acta. 2004 Oct;348(1-2):95-9.
PMID: 15369741 [PubMed - indexed for MEDLINE]

 

Muscarinic receptor

Gil DW, Krauss HA, Bogardus AM, WoldeMussie E.

Muscarinic receptor subtypes in human iris-ciliary body measured by immunoprecipitation.
Invest Ophthalmol Vis Sci. 1997 Jun;38(7):1434-42.
PMID: 9191607 [PubMed - indexed for MEDLINE]

 

Neuron-specific protein

De Graan PN, Dekker LV, Oestreicher AB, Van der Voorn L, Gispen WH.

Determination of changes in the phosphorylation state of the neuron-specific protein kinase C substrate B-50 (GAP43) by quantitative immunoprecipitation.
J Neurochem. 1989 Jan;52(1):17-23.
PMID: 2521182 [PubMed - indexed for MEDLINE]

 

Neutrophil

Lucena-Fernandes F, Dalpe G, Dagenais P, Richard C, Calvert R, Boire G, Menard HA.

Detection of anti-neutrophil cytoplasmic antibodies by immunoprecipitation.
Clin Invest Med. 1995 Jun;18(3):153-62.
PMID: 7554581 [PubMed - indexed for MEDLINE]

 

Nicotinic acetylcholine receptor

Gotti C, Moretti M, Bohr I, Ziabreva I, Vailati S, Longhi R, Riganti L, Gaimarri A, McKeith IG, Perry RH, Aarsland D, Larsen JP, Sher E, Beattie R, Clementi F, Court JA.

Selective nicotinic acetylcholine receptor subunit deficits identified in Alzheimer's disease, Parkinson's disease and dementia with Lewy bodies by immunoprecipitation.
Neurobiol Dis. 2006 Aug;23(2):481-9. Epub 2006 Jun 8.
PMID: 16759874 [PubMed - indexed for MEDLINE]

 

Onconeural antibodies

Monstad SE, Vedeler CA.

An immunoprecipitation assay for the detection of onconeural antibodies.
Acta Neurol Scand Suppl. 2006;183:71-2.
PMID: 16637936 [PubMed - indexed for MEDLINE]

 

Opisthorchis viverrini antigen

Wongratanacheewin S, Chawengkirttikul R, Bunnag D, Sirisinha S.

Analysis of Opisthorchis viverrini antigens by immunoprecipitation and polyacrylamide gel electrophoresis.
Parasitology. 1988 Feb;96 ( Pt 1):119-28.
PMID: 3362572 [PubMed - indexed for MEDLINE]

 

Parathyroid hormone receptor

Wright BS, Tyler GA, O'Brien R, Caporale LH, Rosenblatt M.

Immunoprecipitation of the parathyroid hormone receptor.
Proc Natl Acad Sci U S A. 1987 Jan;84(1):26-30.
PMID: 3025860 [PubMed - indexed for MEDLINE]

 

PDE2

Bentley JK.

Immunoprecipitation of PDE2 phosphorylated and inactivated by an associated protein kinase.
Methods Mol Biol. 2005;307:211-23.
PMID: 15988066 [PubMed - indexed for MEDLINE]

 

Phospholipase D

Gomez-Cambronero J.

Immunoprecipitation of a phospholipase D activity with antiphosphotyrosine antibodies.
J Interferon Cytokine Res. 1995 Oct;15(10):877-85.
PMID: 8564710 [PubMed - indexed for MEDLINE]

 

Placental alkaline phosphatase

Nickson DA, Livingstone JC, Sutcliffe RG.

Detection of the mRNA to placental alkaline phosphatase by in vitro translation and immunoprecipitation.
Dis Markers. 1986 Dec;4(4):283-91.
PMID: 2898318 [PubMed - indexed for MEDLINE]

 

Protein kinase FA/glycogen synthase kinase 3

Van Lint J, Khandelwal RL, Merlevede W, Vandenheede JR.

A specific immunoprecipitation assay for the protein kinase FA/glycogen synthase kinase 3.
Anal Biochem. 1993 Jan;208(1):132-7.
PMID: 8382016 [PubMed - indexed for MEDLINE]

 

Ri antibodies

Knudsen A, Monstad SE, Dorum A, Lonning PE, Salvesen HB, Drivsholm L, Aarseth JH, Vedeler CA.

Ri antibodies in patients with breast, ovarian or small cell lung cancer determined by a sensitive immunoprecipitation technique.
Cancer Immunol Immunother. 2006 Oct;55(10):1280-4. Epub 2006 Jan 21.
PMID: 16429314 [PubMed - indexed for MEDLINE]

 

RNA

Schmitz-Linneweber C, Williams-Carrier R, Barkan A.

RNA immunoprecipitation and microarray analysis show a chloroplast Pentatricopeptide repeat protein to be associated with the 5' region of mRNAs whose translation it activates.
Plant Cell. 2005 Oct;17(10):2791-804. Epub 2005 Sep 2.
PMID: 16141451 [PubMed - indexed for MEDLINE]

 

Steroid 21-hydroxylase autoantibodies

Tanaka H, Perez MS, Powell M, Sanders JF, Sawicka J, Chen S, Prentice L, Asawa T, Betterle C, Volpato M, Smith BR, Furmaniak J.

Steroid 21-hydroxylase autoantibodies: measurements with a new immunoprecipitation assay.
J Clin Endocrinol Metab. 1997 May;82(5):1440-6.
PMID: 9141530 [PubMed - indexed for MEDLINE]

 

SV40 T-antigen

Ludlow JW.

Use of sequential immunoprecipitation to reveal discrete, separable populations of SV40 T-antigen binding to host cellular proteins.
J Virol Methods. 1996 May;59(1-2):105-12.
PMID: 8793836 [PubMed - indexed for MEDLINE]

 

TAR RNA-Binding Protein

Blair ED, Roberts CM, Snowden BW, Gatignol A, Benkirane M, Jeang KT.

Expression of TAR RNA-Binding Protein in Baculovirus and Co-Immunoprecipitation with Insect Cell Protein Kinase.
J Biomed Sci. 1995 Oct;2(4):322-329.
PMID: 11725069 [PubMed - as supplied by publisher]

 

Thyroid microsomal antigen

Kajita Y, Morgan D, Parkes AB, Rees Smith B.

Labelling and immunoprecipitation of thyroid microsomal antigen.
FEBS Lett. 1985 Aug 5;187(2):334-8.
PMID: 3839463 [PubMed - indexed for MEDLINE]

Hamada N, Grimm C, Mori H, DeGroot LJ.

Identification of a thyroid microsomal antigen by Western blot and immunoprecipitation.
J Clin Endocrinol Metab. 1985 Jul;61(1):120-8.
PMID: 3889034 [PubMed - indexed for MEDLINE]

 

Thyrotrophin receptor

de Bruin TW, van der Heide D.

Anti-thyrotrophin receptor antibodies in Graves' disease as demonstrated directly by immunoprecipitation assay.
Acta Endocrinol (Copenh). 1983 Jan;102(1):49-56.
PMID: 6297216 [PubMed - indexed for MEDLINE]

 

TSH-receptor

De Bruin TW, Braverman LE, Brown RS.

Further evaluation of an immunoprecipitation assay for TSH-receptor autoantibodies in Graves' disease.
Metabolism. 1986 Dec;35(12):1101-5.
PMID: 2878349 [PubMed - indexed for MEDLINE]

Konishi J, Iida Y, Kasagi K, Ikekubo K, Kuma K, Torizuka K.

Adipocyte-TSH-receptor-related antibodies in Graves' disease detected by immunoprecipitation.
Endocrinol Jpn. 1982 Apr;29(2):219-26.
PMID: 6127208 [PubMed - indexed for MEDLINE]

 

Xenoestrogens

Natarajan K, Overstreet JW, Rogers JM, Denison MS, Chen J, Lohstroh PN, McConnell DS, Lasley BL.

Detection of xenoestrogens in serum after immunoprecipitation of endogenous steroidal estrogens.
Environ Health Perspect. 2002 Aug;110(8):791-5.
PMID: 12153760 [PubMed - indexed for MEDLINE]

 

Yo antibodies

Monstad SE, Storstein A, Dorum A, Knudsen A, Lonning PE, Salvesen HB, Aarseth JH, Vedeler CA.

Yo antibodies in ovarian and breast cancer patients detected by a sensitive immunoprecipitation technique.
Clin Exp Immunol. 2006 Apr;144(1):53-8.
PMID: 16542365 [PubMed - indexed for MEDLINE]