Antibody Characterization Methods, Techniques & Protocols

Ghosh I, Sun L, Evans TC Jr, Xu MQ.

An improved method for utilization of peptide substrates for antibody characterization and enzymatic assays.
J Immunol Methods. 2004 Oct;293(1-2):85-95.
PMID: 15541279 [PubMed - indexed for MEDLINE]

Jia XC, Raya R, Zhang L, Foord O, Walker WL, Gallo ML, Haak-Frendscho M, Green LL, Davis CG.

A novel method of Multiplexed Competitive Antibody Binning for the characterization of monoclonal antibodies.
J Immunol Methods. 2004 May;288(1-2):91-8.
PMID: 15183088 [PubMed - indexed for MEDLINE]

Oelschlaeger P, Srikant-Iyer S, Lange S, Schmitt J, Schmid RD.

Fluorophor-linked immunosorbent assay: a time- and cost-saving method for the characterization of antibody fragments using a fusion protein of a single-chain antibody fragment and enhanced green fluorescent protein.
Anal Biochem. 2002 Oct 1;309(1):27-34.
PMID: 12381358 [PubMed - indexed for MEDLINE]

Ahluwalia A, De Rossi D, Giusto G, Chen O, Papper V, Likhtenshtein GI.

A fluorescent-photochrome method for the quantitative characterization of solid phase antibody orientation.
Anal Biochem. 2002 Jun 15;305(2):121-34.
PMID: 12054440 [PubMed - indexed for MEDLINE]

Cote HC, Huntsman DG, Wu J, Wadsworth LD, MacGillivray RT.

A new method for characterization and epitope determination of a lupus anticoagulant-associated neutralizing antiprothrombin antibody.
Am J Clin Pathol. 1997 Feb;107(2):197-205.
PMID: 9024068 [PubMed - indexed for MEDLINE]

Bhoopat L, Bhamarapravati N, Attasiri C, Yoksarn S, Chaiwun B, Khunamornpong S, Sirisanthana V.

Immunohistochemical characterization of a new monoclonal antibody reactive with dengue virus-infected cells in frozen tissue using immunoperoxidase technique.
Asian Pac J Allergy Immunol. 1996 Dec;14(2):107-13.
PMID: 9177824 [PubMed - indexed for MEDLINE]

Salgar SK, Paape MJ, Alston-Mills B.

Enzyme-linked immunosorbent assay and flow cytometric methods to screen hybridoma culture supernatants for antibodies to bovine neutrophil surface antigens, and monoclonal antibody production and characterization.
Am J Vet Res. 1993 Sep;54(9):1415-25.
PMID: 8239127 [PubMed - indexed for MEDLINE]

Ito S, Iwasaki A, Syundo J, Tamura Y, Kishi S, Mori H, Ii K, Matsuda Y, Katsunuma N.

Characterization of antibody against human liver guanase by immunoblotting and immunohistochemical staining of human liver guanase by a direct labeling antibody-enzyme method.
J Histochem Cytochem. 1989 May;37(5):611-5.
PMID: 2649557 [PubMed - indexed for MEDLINE]