Gel Shift (Gel)

Antigen-Antibody Specific Applications

 

 

 

E box-binding proteins

Park CW, Walker MD.

Subunit structure of cell-specific E box-binding proteins analyzed by quantitation of electrophoretic mobility shift.
J Biol Chem. 1992 Aug 5;267(22):15642-9.
PMID: 1639803 [PubMed - indexed for MEDLINE]

 

Ceruloplasmin Receptor

Stern RV, Frieden E.

Partial purification of the rat erythrocyte ceruloplasmin receptor monitored by an electrophoresis mobility shift assay.
Anal Biochem. 1993 Jul;212(1):221-8.
PMID: 8396362 [PubMed - indexed for MEDLINE]

 

Calcium-Binding Proteins

Gye MC, Park S, Kim YS, Ahn HS.

Mobility shift assay of calcium-binding proteins of mouse epididymal spermatozoa.
Andrologia. 2001 Jul;33(4):193-8.
PMID: 11472330 [PubMed - indexed for MEDLINE]

 

Endothelin Receptors

Persidis A, Harcombe AA, Davenport AP, Kuc RE, Plumpton C, Weissberg PL.

Isolation of human cardiac endothelin receptors by a peptide-receptor mobility shift assay.
Clin Sci (Lond). 1993 Aug;85(2):169-73.
PMID: 8403786 [PubMed - indexed for MEDLINE]

 

Growth Hormone Gene

Schaufele F, Cassill JA, West BL, Reudelhuber T.

Resolution by diagonal gel mobility shift assays of multisubunit complexes binding to a functionally important element of the rat growth hormone gene promoter.
J Biol Chem. 1990 Aug 25;265(24):14592-8.
PMID: 2387870 [PubMed - indexed for MEDLINE]

 

Glycoproteins

Popov M, Li J, Reithmeier RA.

Resolution of glycoproteins by a lectin gel-shift assay.
Anal Biochem. 2000 Mar 1;279(1):90-5.
PMID: 10683235 [PubMed - indexed for MEDLINE]

 

Heat Shock Protein

Mezger V, Renard JP, Christians E, Morange M.

Detection of heat shock element-binding activities by gel shift assay during mouse preimplantation development.
Dev Biol. 1994 Oct;165(2):627-38.
PMID: 7958427 [PubMed - indexed for MEDLINE]

Levy-Holtzman R, Schechter I.
Schistosome extracts with heat shock factor activity revealed by the gel shift assay.
Parasitology. 1994 Jan;108 ( Pt 1):35-42.
PMID: 8152853 [PubMed - indexed for MEDLINE]

 

IL4-induced nuclear Stat6 activity

Zhang WJ, Thompson JL, Chorney MJ, Koltun WA.

Phenotyping of IL4-induced nuclear Stat6 activity in humans: quantitation after gel shift assay using immortalized cell lines.
Oncol Rep. 2003 Sep-Oct;10(5):1281-8.
PPMID: 12883694 [PubMed - indexed for MEDLINE]

 

Kinetics

Gerstle JT, Fried MG.

Measurement of binding kinetics using the gel electrophoresis mobility shift assay.
Electrophoresis. 1993 Aug;14(8):725-31.
PMID: 8404816 [PubMed - indexed for MEDLINE]

 

Oestrogen Receptor

Koehorst SG, Jacobs HM, Thijssen JH, Blankenstein MA.

Detection of an estrogen receptor-like protein in human meningiomas by band shift assay using a synthetic oestrogen responsive element (ERE).
Br J Cancer. 1993 Aug;68(2):290-4.
PPMID: 8347484 [PubMed - indexed for MEDLINE]

 

Oligodeoxynucleotide triplexes

Shea RG, Ng P, Bischofberger N.

Thermal denaturation profiles and gel mobility shift analysis of oligodeoxynucleotide triplexes.
Nucleic Acids Res. 1990 Aug 25;18(16):4859-66.
PMID: 2395647 [PubMed - indexed for MEDLINE]

 

p53 protein

Pospisilova S, Brazda V, Amrichova J, Kamermeierova R, Palecek E, Vojtesek B.

Precise haracterisation of monoclonal antibodies to the C-terminal region of p53 protein using the PEPSCAN ELISA technique and a new non-radioactive gel shift assay.
J Immunol Methods. 2000 Apr 3;237(1-2):51-64.
PPMID: 10725451 [PubMed - indexed for MEDLINE]

Badiali M, Iolascon A, Loda M, Scheithauer BW, Basso G, Trentini GP, Giangaspero F.

p53 gene mutations in medulloblastoma. Immunohistochemistry, gel shift analysis, and sequencing.
Diagn Mol Pathol. 1993 Mar;2(1):23-8.
PMID: 8287222 [PubMed - indexed for MEDLINE]

 

Peptide Nucleic Acids

Jansen K, Richelson E.

Detection of peptide nucleic acids in tissue extracts of treated animals by gel mobility shift assay.
J Biochem Biophys Methods. 2000 Jan 3;42(1-2):31-4.
PPMID: 10647812 [PubMed - indexed for MEDLINE]

peptide nucleic acids

 

Tetrahymena telomerase

Harrington L, Hull C, Crittenden J, Greider C.

Gel shift and UV cross-linking analysis of Tetrahymena telomerase.
J Biol Chem. 1995 Apr 14;270(15):8893-901.
PPMID: 7721797 [PubMed - indexed for MEDLINE]

 

Transcription factor

Ronai Z, Wang Y, Khandurina J, Budworth P, Sasvari-Szekely M, Wang X, Guttman A.

Transcription factor binding study by capillary zone electrophoretic mobility shift assay.
Electrophoresis. 2003 Jan;24(1-2):96-100.
PMID: 12652578 [PubMed - indexed for MEDLINE]

 

Vibrio anguillarum

Denkin SM, Sekaric P, Nelson DR.

Gel shift analysis of the empA promoter region in Vibrio anguillarum.
BMC Microbiol. 2004 Oct 29;4:42.
PMID: 15516264 [PubMed - indexed for MEDLINE]

 

Vitamin D binding protein (Gc-globulin)

Tang WX, zaraa HM, Magiera H, Cooke NE, Haddad JG.

Electrophoretic mobility shift assay identifies vitamin D binding protein (Gc-globulin) in human, rat, and mouse sera.
Anal Biochem. 1996 Jun 1;237(2):245-51.
PMPMID: 8660573 [PubMed - indexed for MEDLINE]