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Chromatin Immunoprecipitation Methods, Techniques & Protocols
Definition: procedure used to determine whether a given protein binds to or is localized to a specific DNA sequence in vivo.
Antigen-Antibody Specific Applications
Tissue-Cell Specific Applications
ChIP Protocol for Yeast - This protocol is derived from a paper by Miriam Braunstein and is based on work in the Allis lab. The procedure was written by Pam Meluh and updated by Paul Megee (6/10/00).
Chromatin IP (CHIP assay) - This protocol has some minor modification to the protocol described in Strahl-Bolsinger S. et al. [1997, Gen & Dev 11, p83-93] and was obtained from Flick K. (The Scripps Research Institute).
Chromatin Immunoprecipitation (ChIP) Assay - Chromatin Immunoprecipitation (ChIP) assays are used to evaluate the association of proteins with specific DNA regions. The technique involves crosslinking of proteins with DNA, fragmentation and preparation of soluble chromatin followed by immunoprecipitation with an antibody recognizing the protein of interest. The segment of the genome associated with the protein is then identified by PCR amplification of the DNA in the immunoprecipitates. There is a general protocol for ChIP, which is employed by many different labs with minor modifications. Here we describe modified ChIP protocols used successfully in our lab. They include the Standard Protocol, Re-ChIP, Alternative Crosslinking Method and Nucleosome ChIPs.
Chromatin immunoprecipitation on native chromatin from cells and tissues By David Umlauf, Yuji Goto, Katia Delaval, Alexandre Wagschal, Philippe Arnaud and Robert Feil - Institute of Molecular Genetics, Montpellier.
Chromatin Immunoprecipitation Protocol for Histone Modification & Chromatin Associated Proteins By Roderick O'Sullivan & Joost Martens - Jenuwein Lab, Vienna.
Chromatin Immunoprecipitation Protocol to Analyze Histone Modifications in Arabidopsis thaliana By Werner Aufsatz - Matzke Lab, Vienna.
Chromatin Immunoprecipitation (ChIPs) Protocol (Farnham Lab) - This protocol is based upon protocols from Mark Biggin, Dave Allis and Richard Treisman plus a fair amount of trial and error. We have successfully used this protocol with NIH 3T3, Friend, HeLa, Raji and CHO cells. However, you may have to optimize conditions for your specific cell type.
Chromatin Immunoprecipitation (ChIP) Protocol for Tissue - This protocol is based upon protocols from Mark Biggin, Dave Allis and Richard Treisman plus a fair amount of trial and error. We have successfully used this protocol with livers, spleens, colons and whole mouse embryos. However, you may have to optimize conditions for your specific tissue type.