Antibody&Beyond Protocol Guide



A Guide to Multiple Antigen Labeling - In general, immunoenzymatic methods can be used to stain two or more antigens in the same tissue section when the antigens are located in different cell types or different compartments of the same cell. In cases where the two antigens are co-localized in the same compartment of the same cell, e.g., two nuclear antigens, immunofluorescence techniques may be preferred.

Molecular Biology Guide - Ideal for those new to the field or those seeking straightforward, cost effective approaches in optimal labeling and detection of DNA/RNA probes. Instructive diagrams showing labeling methodologies are accompanied by photographic examples submitted by independent investigators that highlights their recent research. Practical insight is offered into choice of labeling method based on probe size, type, application and tag. Subsequent detection of the labeled probes using chromogenic or fluorescent visualization is described for various applications including microarrays, blotting and in situ hybridization. Step-by-step protocols and referenced publications make this a valuable and contemporary reference tool. You may download a copy here. (1.5 MB)

IHC Trouble shooting Guide - A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol as well as issues in weak or no staining. Laminated. You may download a copy here.

Immunoprecipitation - Immunoprecipitation involves the interaction between a protein and its specific antibody, the separation of these immune complexes with Protein G or Protein A, and the subsequent analysis by SDS-PAGE. This technique provides a rapid and simple means to separate a specific protein from whole cell lysates or culture supernatants. Additionally, one can use immunoprecipitation to confirm the identity or study biochemical characteristics, post-translational modifications, and expression levels of a protein of interest. The procedure can be divided into several stages.

Cytokine ELISA Protocol - Due to the amplifying potential of enzyme labels, immunoassays that use enzyme-conjugated antibodies have become increasingly popular because of their high specificity and sensitivity.

Immunohistochemistry Protocol - The protocol below is written for using either purified or biotinylated primary antibodies for immunohistochemical staining of mouse frozen tissue sections.

Light Microscopic Immunocytochemistry Protocol For Tissue Culture - Most of these procedures have been developed by Dr. David M. Sherry of the University of Houston and graciously supplied to Signature Immunologics. We at Signature Immunologics have modified them specifically to address use of our IgGs in detecting amino acids. These protocols are similar to HPI protocols, but some details heve been changed to meet the requirements of working with isolated cells in polystyrene dishes.

Recommended Protocols from AbD Serotec - A set of Protocols and Applications that are recommended for use with AbD Serotec Antibodies.