|
|
Dot Blot Methods, Techniques & Protocols
What is Dot Blot: similar technique as western blotting for detecting proteins in samples that are spotted through circular templates directly onto the membrane.
Antigen-Antibody Specific Applications
Tumor, Disease & Diagnostic Applications
Dot Blot Definition - A technique for detecting, analyzing, and identifying proteins, similar to the Western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane or paper substrate (source: NDI Foundation).
Protocols, troubleshooting and tips for successful dot blotting - Dot blot is a technique for detecting and identifying proteins, similar to the Western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane or paper substrate. Antigens may be applied directly to nitocellulose membrane as a discrete spot (dot) to give a simple and reliable assay.
Immunodot Assay - Goldberg Lab Standard Protocol.
Dot Blot Assay to Quantitate Extent of Biotinylation - Avidity provides a kit to estimate the extent of biotinylation. The kit contains AviTagged Maltose Binding protein (avMBP) in biotinylated (+) and unbiotinylated (-) forms.
Dot-Blot DNA Sexing Procedure - Dot-blot assays for determining the sex of a variety of species of birds were developed in 1990 by Dr. Siwo de Kloet, a former genetics professor at Florida State University, and his wife Dineke de Kloet.