Immuno-Electron Microscopy (IEM)

Antigen Retrieval Methods

 

 

 

Dai W, Sato S, Ishizaki M, Wakamatsu K, Namimatsu S, Sugisaki Y, Ghazizadeh M.

A new antigen retrieval method using citraconic anhydride for immunoelectron microscopy: localization of surfactant pro-protein C (proSP-C) in the type II alveolar epithelial cells.
J Submicrosc Cytol Pathol. 2004 Jul-Oct;36(3-4):219-24.
PMID: 15906596 [PubMed - indexed for MEDLINE]

 

Yano S, Kashima K, Daa T, Urabe S, Tsuji K, Nakayama I, Yokoyama S.

An antigen retrieval method using an alkaline solution allows immunoelectron microscopic identification of secretory granules in conventional epoxy-embedded tissue sections.
J Histochem Cytochem. 2003 Feb;51(2):199-204.
PMID: 12533528 [PubMed - indexed for MEDLINE]

 

Xiao JC, Adam A, Ruck P, Kaiserling E.

A comparison of methods for heat-mediated antigen retrieval for immunoelectron microscopy: demonstration of cytokeratin No. 18 in normal and neoplastic hepatocytes.
Biotech Histochem. 1996 Nov;71(6):278-85.
PMID: 8957553 [PubMed - indexed for MEDLINE]