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Flow Cytometry (FC)
General Methods & Techniques
Bracher M, Gould HJ, Sutton BJ, Dombrowicz D, Karagiannis SN.
Three-colour flow cytometric method to measure
antibody-dependent tumour cell killing by cytotoxicity and
phagocytosis.
J Immunol Methods. 2007 Jun 30;323(2):160-71. Epub 2007 May 15.
PMID: 17531261 [PubMed - in process]
Rosner K, Mischke R, Schuberth HJ.
Evaluation of a standard immunofluorescence assay
and a new flow cytometric method for the detection of autoreactive
antibodies in dogs with tumours.
Res Vet Sci. 2007 Feb;82(1):27-33. Epub 2006
May 22.
PMID: 16716371 [PubMed - indexed for MEDLINE]
Laflamme C, Verreault D, Ho J, Duchaine C.
Flow cytometry sorting protocol of Bacillus spore
using ultraviolet laser and autofluorescence as main sorting
criterion.
J Fluoresc. 2006 Nov;16(6):733-7. Epub 2006
Sep 22.
PMID: 17031569 [PubMed - in process]
Johnson PE, Deromedi AJ, Lebaron P, Catala P, Cash J.
Fountain Flow cytometry, a new technique for the
rapid detection and enumeration of microorganisms in aqueous
samples.
Cytometry A. 2006 Dec 1;69(12):1212-21.
PMID: 17089372 [PubMed - indexed for MEDLINE]
Kantakamalakul W, Pattanapanyasat K, Jongrakthaitae S,
Assawadarachai V, Ampol S, Sutthent R.
A novel EGFP-CEM-NKr flow cytometric method for
measuring antibody dependent cell mediated-cytotoxicity (ADCC)
activity in HIV-1 infected individuals.
J Immunol Methods. 2006 Aug 31;315(1-2):1-10.
Epub 2006 Jul 17.
PMID: 16884734 [PubMed - indexed for MEDLINE]
Petrausch U, Haley D, Miller W, Floyd K, Urba WJ, Walker E.
Polychromatic flow cytometry: a rapid method for
the reduction and analysis of complex multiparameter data.
Cytometry A. 2006 Dec 1;69(12):1162-73.
PMID: 17089357 [PubMed - indexed for MEDLINE]
Montes M, Jaensson EA, Orozco AF, Lewis DE, Corry DB.
A general method for bead-enhanced quantitation
by flow cytometry.
J Immunol Methods. 2006 Dec 20;317(1-2):45-55.
Epub 2006 Oct 10.
PMID: 17067632 [PubMed - indexed for MEDLINE]
The IgE-facilitated allergen binding (FAB) assay:
validation of a novel flow-cytometric based method for the detection
of inhibitory antibody responses.
J Immunol Methods. 2006 Dec 20;317(1-2):71-9.
Epub 2006 Oct 5.
PMID: 17070537 [PubMed - indexed for MEDLINE]
Rapid flow cytometric method for the assessment
of toxic dinoflagellate cyst viability.
Mar Environ Res. 2006 Oct;62(4):247-60. Epub
2006 Apr 18.
PMID: 16740303 [PubMed - indexed for MEDLINE]
Nagy P, Bene L, Hyun WC, Vereb G, Braun M, Antz C, Paysan J,
Damjanovich S, Park JW, Szollsi J.
Novel calibration method for flow cytometric
fluorescence resonance energy transfer measurements between visible
fluorescent proteins.
Cytometry A. 2005 Oct;67(2):86-96.
PMID: 16163690 [PubMed - indexed for MEDLINE]
The use of flow cytometric methods in acute and
long-term in vitro testing.
Toxicol In Vitro. 2005 Oct;19(7):845-52. Epub
2005 Aug 2.
PMID: 16081244 [PubMed - indexed for MEDLINE]
Flow cytometric methods used as screening tests
for basal toxicity of chemicals.
Toxicol In Vitro. 2004 Aug;18(4):483-91.
PMID: 15130606 [PubMed - indexed for MEDLINE]
Three-color labeling method for flow cytometric
measurement of cytogenetic damage in rodent and human blood.
Environ Mol Mutagen. 2004;44(5):427-35.
PMID: 15517570 [PubMed - indexed for MEDLINE]
Tzircotis G, Thorne RF, Isacke CM.
A new spreadsheet method for the analysis of
bivariate flow cytometric data.
BMC Cell Biol. 2004 Mar 22;5:10.
PMID: 15035676 [PubMed - indexed for MEDLINE]
He L, Olson DP, Wu X, Karpova TS, McNally JG, Lipsky PE.
A flow cytometric method to detect
protein-protein interaction in living cells by directly visualizing
donor fluorophore quenching during CFP-->YFP fluorescence resonance
energy transfer (FRET).
Cytometry A. 2003 Oct;55(2):71-85.
PMID: 14505312 [PubMed - indexed for MEDLINE]
Intracellular phospho-protein staining techniques
for flow cytometry: monitoring single cell signaling events.
Cytometry A. 2003 Oct;55(2):61-70.
PMID: 14505311 [PubMed - indexed for MEDLINE]
Nagy S, Jansen J, Topper EK, Gadella BM.
A triple-stain flow cytometric method to assess
plasma- and acrosome-membrane integrity of cryopreserved bovine
sperm immediately after thawing in presence of egg-yolk particles.
Biol Reprod. 2003 May;68(5):1828-35. Epub 2002
Dec 11.
PMID: 12606354 [PubMed - indexed for MEDLINE]
Application of flow cytometric techniques to
veterinary clinical hematology.
Vet Clin Pathol. 2002;31(2):72-82. Review.
PMID: 12040489 [PubMed - indexed for MEDLINE]
Waller A, Pipkorn D, Sutton KL, Linderman JJ, Omann GM.
Validation of flow cytometric competitive binding
protocols and characterization of fluorescently labeled ligands.
Cytometry. 2001 Oct 1;45(2):102-14.
PMID: 11590622 [PubMed - indexed for MEDLINE]
Eschbach E, Reckermann M, John U, Medlin LK.
A simple and highly efficient fixation method for
Chrysochromulina polylepis (Prymnesiophytes) for analytical flow
cytometry.
Cytometry. 2001 Jun 1;44(2):126-32.
PMID: 11378863 [PubMed - indexed for MEDLINE]
Clover LM, Coghill E, Redman CW, Sargent IL.
A three-colour flow cytometry technique for
measuring trophoblast intracellular antigens: the relative
expression of TAP1 in human cytotrophoblast and decidual cells.
Placenta. 2000 Nov;21(8):743-53.
PMID: 11095923 [PubMed - indexed for MEDLINE]
Stabel TJ, Bolin SR, Pesch BA, Rahner TE.
A simple and rapid flow cytometric method for
detection of porcine cell surface markers.
J Immunol Methods. 2000 Nov
1;245(1-2):147-52.
PMID: 11042292 [PubMed - indexed for MEDLINE]
Davey HM, Jones A, Shaw AD, Kell DB.
Variable selection and multivariate methods for
the identification of microorganisms by flow cytometry.
Cytometry. 1999 Feb 1;35(2):162-8.
PMID: 10554172 [PubMed - indexed for MEDLINE]
Millard I, Degrave E, Philippe M, Gala JL.
Detection of intracellular antigens by flow
cytometry: comparison of two chemical methods and microwave heating.
Clin Chem. 1998 Nov;44(11):2320-30.
PMID: 9799760 [PubMed - indexed for MEDLINE]
Kutok JL, Roma AO, Lemire SJ, Dorfman DM.
Four-color flow cytometric immunophenotypic
determination of peripheral blood CD4+ T-lymphocyte counts: a
comparison of validity and cost-effectiveness with a two-color
method.
Am J Clin Pathol. 1998 Oct;110(4):465-70.
PMID: 9763032 [PubMed - indexed for MEDLINE]
Attachment staining: a novel method for flow
cytometric quantitation of protein expression in limited numbers of
adherent cells.
Cytometry. 1998 Jul 1;32(3):198-205.
PMID: 9667509 [PubMed - indexed for MEDLINE]
Serke S, van Lessen A, Huhn D.
Quantitative fluorescence flow cytometry: a
comparison of the three techniques for direct and indirect
immunofluorescence.
Cytometry. 1998 Oct 1;33(2):179-87.
PMID: 9773878 [PubMed - indexed for MEDLINE]
Paterakis GS, Thein SL, Fibach E, Cappellini MD.
Cross evaluation of three flow cytometric F cell
counting methods performed by different laboratories.
Hemoglobin. 1998 Sep-Nov;22(5-6):427-44.
Erratum in: Hemoglobin 1999 Feb;23(1):95.
PMID: 9859927 [PubMed - indexed for MEDLINE]
Measurement of proliferation activities in human
tumor models: a comparison of flow cytometric methods.
Radiat Oncol Investig. 1998;6(3):120-7.
PMID: 9652910 [PubMed - indexed for MEDLINE]
Model MA, KuKuruga MA, Todd RF 3rd.
A sensitive flow cytometric method for measuring
the oxidative burst.
J Immunol Methods. 1997 Mar 28;202(2):105-11.
PMID: 9107299 [PubMed - indexed for MEDLINE]
Mosiman VL, Patterson BK, Canterero L, Goolsby CL.
Reducing cellular autofluorescence in flow
cytometry: an in situ method.
Cytometry. 1997 Jun 15;30(3):151-6.
PMID: 9222101 [PubMed - indexed for MEDLINE]
Lan HY, Hutchinson P, Tesch GH, Mu W, Atkins RC.
A novel method of microwave treatment for
detection of cytoplasmic and nuclear antigens by flow cytometry.
J Immunol Methods. 1996 Mar 28;190(1):1-10.
PMID: 8601701 [PubMed - indexed for MEDLINE]
Greimers R, Trebak M, Moutschen M, Jacobs N, Boniver J.
Improved four-color flow cytometry method using
fluo-3 and triple immunofluorescence for analysis of intracellular
calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and
T-lymphocyte subsets.
Cytometry. 1996 Mar 1;23(3):205-17.
PMID: 8974866 [PubMed - indexed for MEDLINE]
Shaw GR, Gonering E, Koller S.
Techniques to improve flow cytometric detection
of light chain restriction.
Clin Lab Sci. 1996 Sep-Oct;9(5):292-7.
PMID: 10163656 [PubMed - indexed for MEDLINE]
Lillevang ST, Sprogoe-Jakobsen U, Simonsen B, Kristensen T.
Three-colour flow cytometric immunophenotyping in
HIV-patients; comparison to dual-colour protocols.
Scand J Immunol. 1995 Feb;41(2):114-20.
PMID: 7863257 [PubMed - indexed for MEDLINE]
Burdsal CA, Pedersen RA, Hyun WC, Latimer JJ.
Novel flow-cytometric method for separating cell
types in differentiated F9 embryoid bodies.
Cytometry. 1995 Oct 1;21(2):145-52.
PMID: 8582234 [PubMed - indexed for MEDLINE]
Zagursky RJ, Sharp D, Solomon KA, Schwartz A.
Quantitation of cellular receptors by a new
immunocytochemical flow cytometry technique.
Biotechniques. 1995 Mar;18(3):504-9.
PMID: 7779403 [PubMed - indexed for MEDLINE]
Coventry BJ, Neoh SH, Mantzioris BX, Skinner JM, Zola H, Bradley J.
A comparison of the sensitivity of
immunoperoxidase staining methods with high-sensitivity fluorescence
flow cytometry-antibody quantitation on the cell surface.
J Histochem Cytochem. 1994 Aug;42(8):1143-7.
PMID: 8027533 [PubMed - indexed for MEDLINE]
Aubry JP, Shields JG, Jansen KU, Bonnefoy JY.
A multiparameter flow cytometric method to study
surface molecules involved in interactions between subpopulations of
cells.
J Immunol Methods. 1993 Feb
26;159(1-2):161-71.
PMID: 8095276 [PubMed - indexed for MEDLINE]
Flow cytometry: a useful technique in the study
of multidrug resistance.
Biol Cell. 1993;78(1-2):63-8. Review.
PMID: 8106069 [PubMed - indexed for MEDLINE]
Image and flow cytometry: companion techniques
for adherent and non-adherent cell analysis and sorting.
Biol Cell. 1993;78(1-2):129-34.
PMID: 8220223 [PubMed - indexed for MEDLINE]
Clinical applications of flow cytometry.
Immunologic methods for measuring cell membrane and cytoplasmic
antigens.
Cancer. 1992 Mar 15;69(6 Suppl):1543-52.
Review.
PMID: 1540893 [PubMed - indexed for MEDLINE]
Terry NH, White RA, Meistrich ML, Calkins DP.
Evaluation of flow cytometric methods for
determining population potential doubling times using cultured
cells.
Cytometry. 1991;12(3):234-41.
PMID: 2036917 [PubMed - indexed for MEDLINE]
Heiden T, Wang N, Tribukait B.
An improved Hedley method for preparation of
paraffin-embedded tissues for flow cytometric analysis of ploidy and
S-phase.
Cytometry. 1991;12(7):614-21.
PMID: 1723676 [PubMed - indexed for MEDLINE]
Gheuens EE, van Bockstaele DR, van der Keur M, Tanke HJ, van
Oosterom AT, De Bruijn EA.
Flow cytometric double labeling technique for
screening of multidrug resistance.
Cytometry. 1991;12(7):636-44.
PMID: 1685985 [PubMed - indexed for MEDLINE]
A simple method for counting small numbers of
cells by flow cytometry.
Z Naturforsch [C]. 1991
Nov-Dec;46(11-12):1130-3.
PMID: 1817519 [PubMed - indexed for MEDLINE]
Assessment of cellular activation by flow
cytometric methods.
Pathobiology. 1990;58(2):107-17.
PMID: 1972883 [PubMed - indexed for MEDLINE]
Maurer D, Felzmann T, Knapp W.
A single laser flow cytometry method to evaluate
the binding of three antibodies.
J Immunol Methods. 1990 Dec 31;135(1-2):43-7.
PMID: 2148758 [PubMed - indexed for MEDLINE]
Mansour I, Bourin P, Rouger P, Doinel C.
A rapid technique for lymphocyte preparation
prior to two-color immunofluorescence analysis of lymphocyte subsets
using flow cytometry. Comparison with density gradient separation.
J Immunol Methods. 1990 Feb 20;127(1):61-70.
PMID: 2181022 [PubMed - indexed for MEDLINE]
Cavarec L, Quillet-Mary A, Fradelizi D, Conjeaud H.
An improved double fluorescence flow cytometry
method for the quantification of killer cell/target cell conjugate
formation.
J Immunol Methods. 1990 Jul 3;130(2):251-61.
PMID: 2197333 [PubMed - indexed for MEDLINE]
Wing MG, Montgomery AM, Songsivilai S, Watson JV.
An improved method for the detection of cell
surface antigens in samples of low viability using flow cytometry.
J Immunol Methods. 1990 Jan 24;126(1):21-7.
PMID: 2303722 [PubMed - indexed for MEDLINE]
Van Hove L, Goossens W, Van Duppen V, Verwilghen RL.
Reticulocyte count using thiazole orange. A flow
cytometry method.
Clin Lab Haematol. 1990;12(3):287-99.
PMID: 2272158 [PubMed - indexed for MEDLINE]
White RA, Terry NH, Meistrich ML, Calkins DP.
Improved method for computing potential doubling
time from flow cytometric data.
Cytometry. 1990;11(2):314-7.
PMID: 2318085 [PubMed - indexed for MEDLINE]
Kachel V, Kempski O, Peters J, Schodel F.
A method for calibration of flow cytometric
wavelength shift fluorescence measurements.
Cytometry. 1990;11(8):913-5.
PMID: 2272252 [PubMed - indexed for MEDLINE]
Deitch AD, Andreotti VA, Strand MA, Howell L, deVere White RW.
A clinically applicable method to preserve urine
and bladder washing cells for flow cytometric monitoring of bladder
cancer patients.
J Urol. 1990 Apr;143(4):700-5.
PMID: 2179581 [PubMed - indexed for MEDLINE]
Hallden G, Andersson U, Hed J, Johansson SG.
A new membrane permeabilization method for the
detection of intracellular antigens by flow cytometry.
J Immunol Methods. 1989 Nov 13;124(1):103-9.
PMID: 2478636 [PubMed - indexed for MEDLINE]
Terstappen LW, Meiners H, Loken MR.
A rapid sample preparation technique for flow
cytometric analysis of immunofluorescence allowing absolute
enumeration of cell subpopulations.
J Immunol Methods. 1989 Sep 29;123(1):103-12.
PMID: 2477460 [PubMed - indexed for MEDLINE]
Rigg KM, Shenton BK, Murray IA, Givan AL, Taylor RM, Lennard TW.
A flow cytometric technique for simultaneous
analysis of human mononuclear cell surface antigens and DNA.
J Immunol Methods. 1989 Oct 24;123(2):177-84.
PMID: 2809221 [PubMed - indexed for MEDLINE]
Sonneveld P, Schultz FW, Mulder JA.
New method for the analysis of flow cytometric
data.
Anal Quant Cytol Histol. 1988 Aug;10(4):261-8.
PMID: 3166672 [PubMed - indexed for MEDLINE]
Flow cytometry techniques in radiation biology.
Toxicol Lett. 1988 Oct;43(1-3):219-33.
PMID: 3176066 [PubMed - indexed for MEDLINE]
Detection of very low receptor numbers on cells
by flow cytometry using a sensitive staining method.
Cytometry. 1987 Nov;8(6):562-7.
PMID: 2448090 [PubMed - indexed for MEDLINE]
Alberti S, Parks DR, Herzenberg LA.
A single laser method for subtraction of cell
autofluorescence in flow cytometry.
Cytometry. 1987 Mar;8(2):114-9.
PMID: 3556100 [PubMed - indexed for MEDLINE]
Flow cytometry techniques for studying cellular
thiols.
Radiat Res. 1983 Sep;95(3):456-70.
PMID: 6193555 [PubMed - indexed for MEDLINE]
Cells on microspheres: a new technique for flow
cytometric analysis of adherent cells.
Cytometry. 1983 May;3(6):449-52.
PMID: 6343013 [PubMed - indexed for MEDLINE]
An improved sum-of-normals technique for cell
cycle distribution analysis of flow cytometric DNA histograms.
Cell Tissue Kinet. 1981
Jul;14(4):413-23.
PMID: 7020948 [PubMed - indexed for MEDLINE]