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Immunoblotting (IB)
General Methods & Techniques
Immunoblotting techniques.
Methods Mol Biol. 2005;295:227-54.
PMID: 15596900 [PubMed - indexed for MEDLINE]
Hansen KK, Kittok RJ, Sarath G, Beck MM.
Western immunoblotting in avian shell gland
sample immunoblotting methods.
Poult Sci. 2004 Feb;83(2):230-3.
PMID: 14979574 [PubMed - indexed for MEDLINE]
Analysis of the envelope proteins of heat-shocked
Vibrio parahaemolyticus cells by immunoblotting and biotin-labeling
methods.
Microbiol Immunol. 2003;47(5):313-9.
PMID: 12825892 [PubMed - indexed for MEDLINE]
Gilbert C, Rollet-Labelle E, Caon AC, Naccache PH.
Immunoblotting and
sequential lysis protocols for the analysis of tyrosine
phosphorylation-dependent signaling.
J Immunol Methods. 2002 Dec 20;271(1-2):185-201.
PMID: 12445741 [PubMed - indexed for MEDLINE]
Reese G, Schmechel D, Ayuso R, Lehrer SB.
Grid-immunoblotting: a fast and simple technique
to test multiple allergens with small amounts of antibody for
cross-reactivity.
J Chromatogr B Biomed Sci Appl. 2001 May 25;756(1-2):151-6.
PMID: 11419706 [PubMed - indexed for MEDLINE]
Martinez-Garcia JF, Monte E, Quail PH.
A simple, rapid and quantitative method for
preparing Arabidopsis protein extracts for immunoblot analysis.
Plant J. 1999 Oct;20(2):251-7.
PMID: 10571885 [PubMed - indexed for MEDLINE]
Patel S, McLauchlin J, Casemore DP.
A simple SDS-PAGE immunoblotting technique using
an enhanced chemiluminescence detection system to identify
polyclonal antibody responses to complex cryptosporidial antigen
preparations following a monoclonal antibody retest and image
overlay technique.
J Immunol Methods. 1997 Jul 14;205(2):157-61.
PMID: 9294597 [PubMed - indexed for MEDLINE]
Gatti M, Aschbacher R, Cimmino C, Valentini R.
Antigenic profiles for the differentiation of
Mobiluncus curtisii and Mobiluncus mulieris by immunoblotting
technique.
New Microbiol. 1997 Jul;20(3):247-52.
PMID: 9258945 [PubMed - indexed for MEDLINE]
Use of elastase as an internal standard in
immunoblotting techniques.
Biochimie. 1996;78(4):273-6.
PMID: 8874803 [PubMed - indexed for MEDLINE]
A technique for identification of cellular
proteins by western immunoblotting and immunoautoradiography of 2-D
PAGE.
Indian J Biochem Biophys. 1995 Oct;32(5):240-4.
PMID: 8713743 [PubMed - indexed for MEDLINE]
Haury M, Grandien A, Sundblad A, Coutinho A, Nobrega A.
Global analysis of antibody repertoires. 1. An
immunoblot method for the quantitative screening of a large number
of reactivities.
Scand J Immunol. 1994 Jan;39(1):79-87.
PMID: 8290896 [PubMed - indexed for MEDLINE]
Barnes CS, Upadrashta B, Pacheco F, Portnoy J.
Enhanced sensitivity of immunoblotting with
peroxidase-conjugated antibodies using an adsorbed substrate method.
J Chromatogr. 1993 Apr 2;613(2):281-8.
PMID: 8491814 [PubMed - indexed for MEDLINE]
Sato Y, Mukai K, Furuya S, Kameya T, Hirohashi S.
The AMeX method: a multipurpose tissue-processing
and paraffin-embedding method. Extraction of protein and application
to immunoblotting.
Am J Pathol. 1992 Apr;140(4):775-9.
PMID: 1373269 [PubMed - indexed for MEDLINE]
Craig WY, Forster NR, Ledue TB, Johnson AM, Neveux LM, Ritchie RF.
An improved method for determining high molecular
weights by protein immunoblotting: application to apolipoprotein(a)
phenotyping.
Appl Theor Electrophor. 1991;2(4-5):135-40.
PMID: 1782209 [PubMed - indexed for MEDLINE]
Neumann B, Ritter K, Felgenhauer K.
Immunoblot for
densitometric estimation of antibodies (IDEA): a useful method for
quantification of intrathecally produced antibodies against
individual antigens in infectious diseases of the central nervous
system.
J Neuroimmunol. 1991 Nov;34(2-3):173-9.
PMID: 1918324 [PubMed - indexed for MEDLINE]
Comparison of different membranes for use in the
colony-immunoblot technique.
Can J Microbiol. 1990 Mar;36(3):231-5.
PMID: 2187581 [PubMed - indexed for MEDLINE]
Iida R, Yasuda T, Nadano D, Kishi K.
Intensification of peroxidase-diaminobenzidine
staining using gold-sulfide-silver: a rapid and highly sensitive
method for visualization in immunoblotting.
Electrophoresis. 1990 Oct;11(10):852-5.
PMID: 1706659 [PubMed - indexed for MEDLINE]
Checkerboard immunoblotting (CBIB): an efficient,
rapid, and sensitive method of assaying multiple antigen/antibody
cross-reactivities.
J Immunol Methods. 1990 Mar 27;128(1):143-6.
PMID: 2324502 [PubMed - indexed for MEDLINE]
Analysis of proteins adsorbed to glass from human
plasma using immunoblotting methods.
J Biomater Sci Polym Ed. 1990;1(3):173-82.
PMID: 2275919 [PubMed - indexed for MEDLINE]
Nespolo A, Bianchi G, Salmaggi A, Lazzaroni M, Cerrato D, Malesani
Tajoli L.
Immunoblotting techniques
with picogram sensitivity in cerebrospinal fluid protein detection.
Electrophoresis. 1989 Jan;10(1):34-40.
PMID: 2714236 [PubMed - indexed for MEDLINE]
Multiple immunoblot: a sensitive technique to
stain proteins and detect multiple antigens on a single
two-dimensional replica.
Electrophoresis. 1989 Oct;10(10):714-8.
PMID: 2482174 [PubMed - indexed for MEDLINE]
A simplified high speed multicolor immunoblotting
method.
Anal Biochem. 1988 Nov 15;175(1):30-4.
PMID: 2469354 [PubMed - indexed for MEDLINE]
Miniaturization of the immunoblot technique.
Rapid screening for the detection of monoclonal and polyclonal
antibodies.
J Immunol Methods. 1988 Jun 28;111(1):137-41.
PMID: 3392395 [PubMed - indexed for MEDLINE]
Fingerprinting methicillin-resistant
Staphylococcus aureus by the immunoblot technique.
J Med Microbiol. 1988 Apr;25(4):261-8.
PMID: 3357193 [PubMed - indexed for MEDLINE]
Immunoblotting and dot
immunobinding. Emerging techniques in protein immunochemistry.
Arch Pathol Lab Med. 1988 Aug;112(8):780-6.
PMID: 3395213 [PubMed - indexed for MEDLINE]
Lefevre M, Goudey-Lefevre JC, Roheim PS.
Gradient gel electrophoresis-immunoblot analysis
(GGEI): a sensitive method for apolipoprotein profile
determinations.
J Lipid Res. 1987 Dec;28(12):1495-507.
PMID: 3123587 [PubMed - indexed for MEDLINE]
Immunoblot analysis: a new
method for fingerprinting hospital pathogens.
J Immunol Methods. 1987 Jun 26;100(1-2):41-6. Review.
PMID: 3298439 [PubMed - indexed for MEDLINE]
Wiederkehr F, Ogilvie A, Vonderschmitt DJ.
Cerebrospinal fluid proteins studied by
two-dimensional gel electrophoresis and immunoblotting technique.
J Neurochem. 1987 Aug;49(2):363-72.
PMID: 3598577 [PubMed - indexed for MEDLINE]
Immunoblotting: an emerging
technique in immunohematology.
Vox Sang. 1986;50(3):129-36. Review.
PMID: 2940748 [PubMed - indexed for MEDLINE]
A simple immunoblotting method after separation
of proteins in agarose gel.
J Immunol Methods. 1985 Nov 28;84(1-2):271-8.
PMID: 3905970 [PubMed - indexed for MEDLINE]
Electroimmunoassay-immunoblotting (EIA-IB) for the utilization of
monoclonal antibodies in quantitative immunoelectrophoresis: the
method and its applications.
J Immunol Methods. 1985 Jun 12;80(1):25-32.
PMID: 4008937 [PubMed - indexed for MEDLINE]
A sensitive immunoblotting method for measuring
protein synthesis initiation factor levels in lysates of Escherichia
coli.
J Biol Chem. 1981 Dec 25;256(24):12836-9.
PMID: 7031052 [PubMed - indexed for MEDLINE]