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Immunofluorescence (IF)
Multiple Labeling Methods & Techniques
Pineau I, Barrette B, Vallires N, Lacroix S.
A novel method for multiple labeling combining in
situ hybridization with immunofluorescence.
J Histochem Cytochem. 2006 Nov;54(11):1303-13. Epub 2006 Aug 9.
PMID: 16899759 [PubMed - indexed for MEDLINE]
Suzuki T, Tate G, Ikeda K, Mitsuya T.
A novel multicolor immunofluorescence method
using heat treatment.
Acta Med Okayama. 2005 Aug;59(4):145-51.
PMID: 16155640 [PubMed - indexed for MEDLINE]
An innovative fixative for cytoskeletal
components allows high resolution in colocalization studies using
immunofluorescence techniques.
Histochem Cell Biol. 2004 Jul;122(1):1-5. Epub 2004 Jun 8.
PMID: 15185086 [PubMed - indexed for MEDLINE]
Schieker M, Pautke C, Reitz K, Hemraj I, Neth P, Mutschler W, Milz
S.
The use of four-colour
immunofluorescence techniques to identify mesenchymal stem cells.
J Anat. 2004 Feb;204(2):133-9.
PMID: 15032920 [PubMed - indexed for MEDLINE]
Uchihara T, Nakamura A, Yamazaki M, Mori O, Ikeda K, Tsuchiya K.\
Different conformation of neuronal tau deposits
distinguished by double immunofluorescence with AT8 and thiazin red
combined with Gallyas method.
Acta Neuropathol (Berl). 2001 Nov;102(5):462-6.
PMID: 11699559 [PubMed - indexed for MEDLINE]
Uchihara T, Nakamura A, Yamazaki M, Mori O.
Evolution from pretangle neurons to
neurofibrillary tangles monitored by thiazin red combined with
Gallyas method and double immunofluorescence.
Acta Neuropathol (Berl). 2001 Jun;101(6):535-9.
PMID: 11515780 [PubMed - indexed for MEDLINE]
A method for measuring colocalization of
presynaptic markers with anatomically labeled axons using double
label immunofluorescence and confocal microscopy.
J Neurosci Methods. 2000 Jan 15;94(2):205-15. Erratum in: J Neurosci
Methods 2000 Mar 1;96(1):87.
PMID: 10661840 [PubMed - indexed for MEDLINE]
Wang G, Achim CL, Hamilton RL, Wiley CA, Soontornniyomkij V.
Tyramide signal
amplification method in multiple-label immunofluorescence confocal
microscopy.
Methods. 1999 Aug;18(4):459-64.
PMID: 10491275 [PubMed - indexed for MEDLINE]
Kroeber S, Schomerus C, Korf HW.
A specific and sensitive double-immunofluorescence
method for the demonstration of S-antigen and serotonin in trout and
rat pinealocytes by means of primary antibodies from the same donor
species.
Histochem Cell Biol. 1998 Apr;109(4):309-17.
PMID: 9562380 [PubMed - indexed for MEDLINE]
de Bont ES, Niemarkt AE, Tamminga RY, Kimpen JL, Kamps WA, de Leij
LH.
Lipopolysaccharide-induced
cytokine production in peripheral blood mononuclear cells:
intracellular localization of tumor necrosis factor alpha and
interleukin 1 beta detected with a three-color immunofluorescence
technique.
Histochem Cell Biol. 1996 Dec;106(6):593-8.
PMID: 8985748 [PubMed - indexed for MEDLINE]
Simultaneous application of Timm's sulphide
silver method and immunofluorescence histochemistry.
J Neurosci Methods. 1993 Jun;48(1-2):149-56.
PMID: 8377517 [PubMed - indexed for MEDLINE]
Gazitt Y, He YJ, Erdos GW, Chang L, Ashktorab H, Cohen RJ.
Development of a two color immunofluorescence
stain and immunolocalization method for N-myc and c-myc oncoproteins
with a newly generated mouse IgM anti N-myc antibody.
J Immunol Methods. 1992 Apr 8;148(1-2):159-69.
PMID: 1564326 [PubMed - indexed for MEDLINE]
Wessendorf MW, Appel NM, Molitor TW, Elde RP.
A method for immunofluorescent demonstration of
three coexisting neurotransmitters in rat brain and spinal cord,
using the fluorophores fluorescein, lissamine rhodamine, and
7-amino-4-methylcoumarin-3-acetic acid.
J Histochem Cytochem. 1990 Dec;38(12):1859-77.
PMID: 1701460 [PubMed - indexed for MEDLINE]
A triple-labeling method: HRP anterograde tract
tracing combined with double immunofluorescent cell staining in
developing neural tissue of the rat.
J Neurosci Methods. 1989 Oct;30(1):71-6.
PMID: 2811435 [PubMed - indexed for MEDLINE]
Dziwisch L, Heesemann J, Kremer B.
The microscopic double immunofluorescence
technique, a method for quantitative differentiation between extra-
and intracellularly located bacteria in isolated polymorphonuclear
granulocytes.
Med Microbiol Immunol. 1988;177(2):101-7.
PMID: 3283509 [PubMed - indexed for MEDLINE]
Dziwisch L, Heesemann J, Kremer B.
The microscopic double immunofluorescence
technique, a method for quantitative differentiation between extra-
and intracellularly located bacteria in isolated polymorphonuclear
granulocytes.
Med Microbiol Immunol. 1988;177(2):101-7.
PMID: 3283509 [PubMed - indexed for MEDLINE]
Larson BA, Bern HA, Lin RJ, Nishioka RS.
A double sequential immunofluorescence method
demonstrating the co-localization of urotensins I and II in the
caudal neurosecretory system of the teleost, Gillichthys mirabilis.
Cell Tissue Res. 1987 Feb;247(2):233-9.
PMID: 3545481 [PubMed - indexed for MEDLINE]
A new double labelling
immunofluorescence technique for the determination of proliferation
of human astrocytes in culture.
J Neurosci Methods. 1987 Sep;21(1):9-16.
PMID: 3309485 [PubMed - indexed for MEDLINE]
Two embryonic, tissue-specific molecules
identified by a double-label immunofluorescence technique for
monoclonal antibodies.
J Histochem Cytochem. 1986 Jun;34(6):703-6.
PMID: 3084626 [PubMed - indexed for MEDLINE]
Double staining technique
using a combination of indirect and direct immunofluorescence with
monoclonal antibodies.
Acta Histochem. 1986;78(2):185-8.
PMID: 2425537 [PubMed - indexed for MEDLINE]
Double immunofluorescence
microscopic technique for accurate differentiation of
extracellularly and intracellularly located bacteria in cell
culture.
J Clin Microbiol. 1985 Aug;22(2):168-75.
PMID: 3897263 [PubMed - indexed for MEDLINE]
Lechago J, Sun NC, Weinstein WM.
Simultaneous visualization
of two antigens in the same tissue section by combining
immunoperoxidase with immunofluorescence techniques.
J Histochem Cytochem. 1979 Sep;27(9):1221-5.
PMID: 113453 [PubMed - indexed for MEDLINE]