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Immunofluorescence (IF)
Tissue-Cell Specific Applications
Antineutrophil cytoplasmic antibodies
Detection of antineutrophil cytoplasmic
antibodies in primary sclerosing cholangitis: a comparison of the
alkaline phosphatase and immunofluorescent techniques.
Eur J Gastroenterol Hepatol. 1997
Jun;9(6):575-80. Erratum in: Eur J Gastroenterol Hepatol 1997
Dec;9(12):1257.
PMID: 9222729 [PubMed - indexed for MEDLINE]
Bacilli
Tomov A, Tsanev N, Tsvetkova E.
Specific staining of bacilli by means of immuno-indian-ink
method and immunofluorescent method.
Zentralbl Bakteriol [Orig A]. 1979
Mar;243(1):119-24.
PMID: 87066 [PubMed - indexed for MEDLINE]
Bacillus spores
Variations on the staining
method in quantitative indirect immunofluorescence assays for
Bacillus spores, and the use of fluorescein--protein A.
J Immunol Methods. 1982 Nov 12;54(3):361-9.
PMID: 6184416 [PubMed - indexed for MEDLINE]
Blood
Simple immunofluorescent method for
identification of blood group in human tissues.
Acta Histochem. 1985;77(2):209-11.
PMID: 3938173 [PubMed - indexed for MEDLINE]
Brutel de la Riviere A, Verhoef-Karssen PR, Bosma A, Kr vd Borne AE,
Engelfriet P.
Specific antisera against human blood cells
applicable in the indirect immunofluorescence technique.
Scand J Immunol. 1976;5(9):1065-74.
PMID: 795002 [PubMed - indexed for MEDLINE]
Blood vessel
Kittelberger R, Davis PF, Stehbens WE.
An improved immunofluorescence technique for
the histological examination of blood vessel tissue.
Acta Histochem. 1989;86(2):137-42.
PMID: 2481931 [PubMed - indexed for MEDLINE]
Brain
Umoh JU, Ezeokoli CD, Okoh AE.
Immunofluorescent staining of trypsinized
formalin-fixed brain smears for rabies antigen: results compared
with those obtained by standard methods for 221 suspect animal cases
in Nigeria.
J Hyg (Lond). 1985 Feb;94(1):129-34.
PMID: 2982942 [PubMed - indexed for MEDLINE]
The application of immunofluorescent antibody
method in research of human brain edema.
Neurosci Lett. 1980 May
1;17(3):323-7.
PMID: 6763164 [PubMed - indexed for MEDLINE]
A specific immunofluorescence technique for the
demonstration of vasogenic brain edema in paraffin embedded
material.
Acta Neuropathol (Berl). 1979 Jan
12;45(1):47-51.
PMID: 367045 [PubMed - indexed for MEDLINE]
Simultaneous immunofluorescence and
autoradiography: a useful technique for investigating
neurotransmitter uptake by neurons and glia in primary central
nervous system culture.
Brain Res. 1987 Nov;433(1):1-11.
PMID: 3315123 [PubMed - indexed for MEDLINE]
Imprinting as a rapid technique for assessing
the morphology of the central nervous system by immunofluorescence.
J Neurosci Methods. 2000 Jul
31;100(1-2):85-91.
PMID: 11040370 [PubMed - indexed for MEDLINE]
A method for specific
transmitter identification of retrogradely labeled neurons:
immunofluorescence combined with fluorescence tracing.
Brain Res. 1984 Dec;320(2-3):99-127.
PMID: 6084535 [PubMed - indexed for MEDLINE]
Chlamydia trachomatis
Ciotti RA, Sondheimer SJ, Nachamkin I.
Detecting Chlamydia trachomatis by direct
immunofluorescence using a Cytobrush sampling technique.
Genitourin Med. 1988 Aug;64(4):245-6.
Erratum in: Genitourin Med 1989 Jan;65(1):62.
PMID: 3049302 [PubMed - indexed for MEDLINE]
Ciliated protozoans
An immunofluorescence technique for staining
ciliated protozoans: highlighting cytoplasmic microtubular arrays
and stages of micronuclear meiosis.
Micron. 2001 Feb;32(2):207-10.
PMID: 10936463 [PubMed - indexed for MEDLINE]
Cochlea
An improved technique for the study of
immunofluorescence using non-decalcified frozen guinea pig cochlea.
J Laryngol Otol. 1988
Mar;102(3):215-8.
PMID: 3282016 [PubMed - indexed for MEDLINE]
Dictyostelium cell
Hagedorn M, Neuhaus EM, Soldati T.
Optimized fixation and
immunofluorescence staining methods for Dictyostelium cells.
Methods Mol Biol. 2006;346:327-38.
PMID: 16957300 [PubMed - indexed for MEDLINE]
DNA
Clevenger CV, Bauer KD, Epstein AL.
A method for simultaneous nuclear
immunofluorescence and DNA content quantitation using monoclonal
antibodies and flow cytometry.
Cytometry. 1985 May;6(3):208-14.
PMID: 3888556 [PubMed - indexed for MEDLINE]
Endocrine cell
L'Hermite A, Ferrand R, Dubois MP, Andersen AC.
Detection of endocrine cells by
immunofluorescence method in the gastroenteropancreatic system of
the adult eel, glass-eel, and leptocephalic larva (Anguilla anguilla
L.).
Gen Comp Endocrinol. 1985 Jun;58(3):347-59.
PMID: 2861142 [PubMed - indexed for MEDLINE]
Endomyocardial biopsy
Hammond EH, Hansen JK, Spencer LS, Jensen A, Yowell RL.
Immunofluorescence of endomyocardial biopsy
specimens: methods and interpretation.
J Heart Lung Transplant. 1993
Mar-Apr;12(2):S113-24.
PMID: 8476881 [PubMed - indexed for MEDLINE]
Endothelium
Biopsy coupled to quantitative immunofluorescence:
a new method to study the human vascular endothelium.
J Appl Physiol. 2002 Mar;92(3):1331-8.
PMID: 11842075 [PubMed - indexed for MEDLINE]
Entamoeba histolytica
Gilman R, Islam M, Paschi S, Goleburn J, Ahmad F.
Comparison of conventional and
immunofluorescent techniques for the detection of Entamoeba
histolytica in rectal biopsies.
Gastroenterology. 1980
Mar;78(3):435-9.
PMID: 6153164 [PubMed - indexed for MEDLINE]
Eosinophils
Jahnsen FL, Haraldsen G, Rugtveit J, Halstensen TS, Brandtzaeg P.
Differential interference contrast microscopy
combined with immunofluorescence: a new method to phenotype
eosinophils in situ.
J Immunol Methods. 1994 Jul 12;173(1):77-91.
PMID: 8034989 [PubMed - indexed for MEDLINE]
Fetomaternal haemorrhage
Detection of fetomaternal haemorrhage by an
immunofluorescence technique.
J Clin Pathol. 1985 Aug;38(8):919-20.
PMID: 3928702 [PubMed - indexed for MEDLINE]
Fibroblasts
Abuknesha RA, al-Mazeedi HM, Price RG.
AMC-anti-FITC conjugates: novel reagents for amplified
immunochemical techniques. Immunofluorescent staining of human
fibroblasts.
Histochem J. 1992 Sep;24(9):655-62.
PMID: 1385365 [PubMed - indexed for MEDLINE]
Kinetochore
immunofluorescence in micronuclei: a rapid method for the in situ
detection of aneuploidy and chromosome breakage in human
fibroblasts.
Mutat Res. 1988 Dec;203(6):405-14.
PMID: 3057373 [PubMed - indexed for MEDLINE]
Fungi
Immunofluorescence method
for the detection of fungi in bronchial secretions
Z Erkr Atmungsorgane. 1983;160(2):157-60. German.
PMID: 6344455 [PubMed - indexed for MEDLINE]
Glial Cell
Bajetto A, Barbero S, Bonavia R, Chimini G, Schettini G.
Immunofluorescence and biochemical techniques
to detect nuclear localization of ciliary neurotrophic factor in
glial cells.
Brain Res Brain Res Protoc. 2000
Jul;5(3):273-81.
PMID: 10906493 [PubMed - indexed for MEDLINE]
Granulocytes
An immunofluorescent method for a specific
demonstration of granulocytes and some of their proteins (ECP and
CCP).
Histochemistry. 1986;84(3):247-50.
PMID: 3519546 [PubMed - indexed for MEDLINE]
Schmidt-Melbye A, Kolstad A, Hannestad K.
Antibodies to granulocytes detected by an
indirect immunofluorescence method not requiring chemical
modification of cells.
Transfusion. 1985 Mar-Apr;25(2):165-9.
PMID: 3885485 [PubMed - indexed for MEDLINE]
Heart
Yamamoto A, Wenthold RJ Jr, Zhang J, Herman EH, Ferrans VJ.
Immunofluorescence techniques for the
identification of immune effector cells in rat heart: applications
to the study of the myocarditis induced by interleukin-2.
J Mol Cell Cardiol. 1995
Jan;27(1):307-19.
PMID: 7539083 [PubMed - indexed for MEDLINE]
Immunoglobulin-producing cells
Immunoglobulin-producing cells in lymphatic
tissues of germfree and conventional rabbits as detected by an
immunofluorescence method.
Folia Microbiol (Praha). 1985;30(3):291-4.
PMID: 4040051 [PubMed - indexed for MEDLINE]
Islet cell
Stefan Y, Malaisse-Lagae F, Yoon JW, Notkins AL, Orci L.
Virus-induced diabetes in mice: a quantitative
evaluation of islet cell population by immunofluorescence technique.
Diabetologia. 1978 Nov;15(5):395-401.
PMID: 216598 [PubMed - indexed for MEDLINE]
Kobayashi T, Sugimoto T, Itoh T, Kosaka K, Tanaka T, Suwa S, Sato K,
Tsuji K.
The prevalence of islet cell antibodies in
Japanese insulin-dependent and non-insulin-dependent diabetic
patients studied by indirect immunofluorescence and by a new method.
Diabetes. 1986 Mar;35(3):335-40.
PMID: 3512344 [PubMed - indexed for MEDLINE]
Keratinocyte
Okumura H, Matsumto K, Hashimoto K, Yoshikawa K.
A new method for quantifying keratinocyte
differentiation using immunofluorescent staining of involucrin and
cytofluorography.
Exp Cell Res. 1991 Feb;192(2):647-50.
PMID: 1988301 [PubMed - indexed for MEDLINE]
Cirillo N, Femiano F, Dell'ermo A, Arnese P, Gombos F, Lanza A.
A novel method to investigate pemphigus-induced
keratinocyte dysmorphisms through living cell immunofluorescence
microscopy.
Virchows Arch. 2007
Jun;450(6):683-90. Epub 2007 Apr 21.
PMID: 17450380 [PubMed - in process]
Kidney
New technique of kidney tissue processing for
immunofluorescence microscopy: formol sucrose/gum sucrose/paraffin.
Lab Invest. 1982 Aug;47(2):206-13.
PMID: 7050531 [PubMed - indexed for MEDLINE]
Lipid droplets
Fixation methods for the
study of lipid droplets by immunofluorescence microscopy.
J Histochem Cytochem. 2003 Jun;51(6):773-80.
PMID: 12754288 [PubMed - indexed for MEDLINE]
Lung
Trotman W, Beckett T, Goncz KK, Beatty BG, Weiss DJ.
Dual Y chromosome painting and in situ
cell-specific immunofluorescence staining in lung tissue: an
improved method of identifying donor marrow cells in lung following
bone marrow transplantation.
Histochem Cell Biol. 2004 Jan;121(1):73-9. Epub 2003 Dec 12.
PMID: 14673657 [PubMed - indexed for MEDLINE]
Lymphocytes
Laskay T, Andersson U, Andersson J, Kiessling R, DeLey M.
An immunofluorescent method for identifying
individual IFN-gamma-producing lymphocytes.
J Immunol Methods. 1986 Dec
4;95(1):1-7.
PMID: 2431063 [PubMed - indexed for MEDLINE]
Korcakova L, Salkova J, Lejnar J, Kaslik J, Raska I.
Labelling of a T lymphocyte
subpopulation with monoclonal antibody bound to colloidal gold.
Correlation with the indirect immunofluorescence method.
Folia Biol (Praha). 1988;34(4):209-14.
PMID: 3071478 [PubMed - indexed for MEDLINE]
An immunofluorescent technique for the
detection of lymphocyte alloantigens.
J Immunol Methods.
1977;17(1-2):57-61.
PMID: 333028 [PubMed - indexed for MEDLINE]
Janossy G, Thomas JA, Pizzolo G.
The analysis of lymphoid subpopulations in
normal and malignant tissues by immunofluorescence techniques.
J Cancer Res Clin Oncol.
1981;101(1):1-11.
PMID: 6456270 [PubMed - indexed for MEDLINE]
Chollet-Martin S, Gougerot-Pocidalo MA.
Enumeration of the T4 positive and T8 positive
lymphocytes in AIDS and related syndromes. Comparison of the
immunogold and the immunofluorescence techniques.
J Immunol Methods. 1986 Jul
24;91(2):271-4.
PMID: 3090151 [PubMed - indexed for MEDLINE]
A technique for simultaneous antikinetochore
immunofluorescence staining and Q-banding in chromosomes from human
lymphocytes.
Stain Technol. 1987 Jul;62(4):221-5.
PMID: 2442860 [PubMed - indexed for MEDLINE]
Mills K, Armitage R, Worman C.
An indirect rosette technique for the
identification and separation of human lymphocyte populations by
monoclonal antibodies: a comparison with immunofluorescence methods.
Immunol Lett. 1983 May;6(5):241-6.
PMID: 6224744 [PubMed - indexed for MEDLINE]
Bourguignon LY, Tokuyasu KT, Singer SJ.
The capping of lymphocytes
and other cells, studied by an improved method for
immunofluorescence staining of frozen sections.
J Cell Physiol. 1978 Jun;95(3):239-57.
PMID: 77276 [PubMed - indexed for MEDLINE]
Wybran J, Rosenberg J, Romasco F.
Immunogold staining: an
alternative method for lymphocyte subset enumeration. Comparison
with immunofluorescence microscopy and flow cytometry.
J Immunol Methods. 1985 Feb 11;76(2):229-38.
PMID: 3882835 [PubMed - indexed for MEDLINE]
Macrophage
Nakstad B, Lyberg T, Baklien K, Boye NP.
Antigenic specificity of human alveolar
macrophages and blood monocytes studied by an immunofluorescence
technique.
J Pathol. 1989 Feb;157(2):99-107.
PMID: 2466114 [PubMed - indexed for MEDLINE]
Mast Cell
Botchkarev VA, Eichmuller S, Peters EM, Pietsch P, Johansson O,
Maurer M, Paus R.
A simple immunofluorescence technique for
simultaneous visualization of mast cells and nerve fibers reveals
selectivity and hair cycle--dependent changes in mast cell--nerve
fiber contacts in murine skin.
Arch Dermatol Res. 1997
Apr;289(5):292-302.
PMID: 9164640 [PubMed - indexed for MEDLINE]
Microtubules
Simmonds DH, Seagull RW, Setterfield G.
Evaluation of techniques for immunofluorescent
staining of microtubules in cultured plant cells.
J Histochem Cytochem. 1985
Apr;33(4):345-52.
PMID: 2579999 [PubMed - indexed for MEDLINE]
Motor endplates
Sondag-Tschroots IR, Schulz-Raateland RC, van Walbeek HK, Feltkamp
TE.
Antibodies to motor endplates demonstrated with
the immunofluorescence technique.
Clin Exp Immunol. 1979
Aug;37(2):323-7.
PMID: 387311 [PubMed - indexed for MEDLINE]
Mycoplasma colonies
Bradbury JM, Oriel CA, Jordan FT.
Simple method for immunofluorescent
identification of mycoplasma colonies.
J Clin Microbiol. 1976
Apr;3(4):449-52.
PMID: 770501 [PubMed - indexed for MEDLINE]
Mycoplasma hominis
Hirai Y, Kanatani T, Ono M, Matsushita O, Kanemasa Y.
An indirect immunofluorescence method for
detection of Mycoplasma hominis in vaginal smears.
Microbiol Immunol. 1991;35(10):831-9.
PMID: 1779888 [PubMed - indexed for MEDLINE]
Neutrophil
McKane AM, McMillan SA, Conlan SK, McNeill TA.
Comparison of immunofluorescence and an
immunohistochemical technique for the detection of perinuclear anti-neutrophil
cytoplasmic antibody.
Ir J Med Sci. 1991 Mar;160(3):93-5.
PMID: 1717399 [PubMed - indexed for MEDLINE]
Ocular fundus
Scheiffarth OF, Zrenner E, Disko R, Stefani FH, Brabander B.
Intraocular in vivo immunofluorescence. A new
technique for visualizing structures of the ocular fundus.
Invest Ophthalmol Vis Sci. 1990
Feb;31(2):272-6.
PMID: 2105916 [PubMed - indexed for MEDLINE]
Placenta
Ali Akbar S, Nicolaides KH, Brown PR.
The localisation of copper/zinc superoxide
dismutase in human normal term placenta by an immunofluorescence
technique.
J Obstet Gynaecol. 1998
Jan;18(1):44-6.
PMID: 15512001 [PubMed]
Plasma cell
Kingston D, Pearson JR, Penna FJ.
Plasma cell counts on human jejunal biopsy
specimens examined by immunofluorescence and immunoperoxidase
techniques: a comparative study.
J Clin Pathol. 1981 Apr;34(4):381-5.
PMID: 7016923 [PubMed - indexed for MEDLINE]
Pleural fluid cell
Keyhani-Rofagha S, O'Toole R, Meister RK, Neff J.
Identification of cultured pleural fluid cells
by a direct immunofluorescence technique.
Anal Quant Cytol. 1984
Dec;6(4):238-42.
PMID: 6397083 [PubMed - indexed for MEDLINE]
Pneumocystis carinii
Orholm M, Holten-Andersen W, Lundgren JD.
Improved detection of Pneumocystis carinii by
an immunofluorescence technique using monoclonal antibodies.
Eur J Clin Microbiol Infect Dis. 1990
Dec;9(12):880-5.
PMID: 2073898 [PubMed - indexed for MEDLINE]
Proliferating cell
Combined immunohistochemical and
immunofluorescence method to determine the phenotype of
proliferating cell populations.
J Clin Pathol. 1992 Nov;45(11):1015-7. Erratum in: J Clin Pathol
1992 Dec;45(12):1124.
PMID: 1485903 [PubMed - indexed for MEDLINE]
Rickettsia rickettsii
Walker DH, Harrison A, Henderson F, Murphy FA.
Identification of Rickettsia rickettsii in a
guinea pig model by immunofluorescent and electron microscopic
techniques.
Am J Pathol. 1977 Feb;86(2):343-58.
PMID: 402079 [PubMed - indexed for MEDLINE]
Reticulocytes
Seligman PA, Allen RH, Kirchanski SJ, Natale PJ.
Automated analysis of reticulocytes using
fluorescent staining with both acridine orange and an
immunofluorescence technique.
Am J Hematol. 1983 Feb;14(1):57-66.
PMID: 6301264 [PubMed - indexed for MEDLINE]
Spermatozoa
A technique for preserving retained distal
cytoplasmic droplets in situ for immunofluorescence evaluation of
ejaculated porcine spermatozoa.
Prep Biochem Biotechnol. 2003
Nov;33(4):301-10.
PMID: 14606687 [PubMed - indexed for MEDLINE]
Streptococcus mutans
Bush MS, Challacombe SJ, Newman HN.
A method for the identification of Streptococcus
mutans in gingival margin plaque by immunofluorescence.
Caries Res. 1990;24(1):23-9.
PMID: 1688401 [PubMed - indexed for MEDLINE]
T Cell
Patel R, Williams C, Patel DR.
T cell and T cell subset determination in
normal peripheral blood: comparison of the indirect
immunofluorescence and lymphocytotoxicity techniques.
Experientia. 1984 Dec
15;40(12):1412-3.
PMID: 6239789 [PubMed - indexed for MEDLINE]
Thoracic aorta
Investigation of relationships between
collagens, elastin and proteoglycans in bovine thoracic aorta by
immunofluorescence techniques.
Histochem J. 1983 Dec;15(12):1177-90.
PMID: 6358143 [PubMed - indexed for MEDLINE]
Thyrotropic cells
Tal E, Savion S, Abraham M, Hanna N.
An immunofluorescent method for identification
of isolated thyrotropic cells.
Experientia. 1978 Apr 15;34(4):536-7.
PMID: 346370 [PubMed - indexed for MEDLINE]