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Immunoprecipitation (IP)
Antigen-Antibody Specific Applications
5C3
Larkin A, Moran E, Kennedy SM, Clynes M.
Monoclonal antibody 5C3
raised against formalin fixed paraffin-embedded invasive breast
tumour tissue: characterisation of its reactive antigen via
immunoprecipitation and internal sequencing.
J Immunol
Methods. 2005 Aug;303(1-2):53-65.
PMID: 16038928 [PubMed - indexed for MEDLINE]
170-kDa bullous pemphigoid antigen
Ebihara T, Hashimoto T, Kudoh J, Gamou S, Shimizu N, Nishikawa T.
Detection of the 170-kDa bullous pemphigoid antigen by
immunoprecipitation.
J Invest Dermatol. 1993 Feb;100(2):176-9.
PMID: 8429239 [PubMed - indexed for MEDLINE]
A1 adenosine receptor-GTP-binding protein
Immunoprecipitation of A1
adenosine receptor-GTP-binding protein complexes in ciliary
epithelial cells.
Invest Ophthalmol Vis Sci. 1994 Jun;35(7):3057-63.
PMID: 8206723 [PubMed - indexed for MEDLINE]
AA4
Stephan V, Guo N, Ginsburg V, Siraganian RP.
Immunoprecipitation of membrane proteins from rat basophilic
leukemia cells by the antiganglioside monoclonal antibody AA4.
J Immunol. 1991 Jun 15;146(12):4271-7.
PMID: 1828263 [PubMed - indexed for MEDLINE]
ABH
Sato I, Hamabe T, Yamazaki K, Watanabe Y.
A new method for the determination of the ABO
blood type of semen by immunoblotting using anti-ABH antibodies
following immunoprecipitation.
J Immunol Methods. 1995 Dec 27;188(2):229-37.
PMID: 8551051 [PubMed - indexed for MEDLINE]
Albumin
Improved method for the specific
immunoprecipitation of albumin.
Mol Cell Biochem. 1979 Oct 15;27(2):85-8.
PMID: 388187 [PubMed - indexed for MEDLINE]
Amyloid beta
Clarke NJ, Tomlinson AJ, Ohyagi Y, Younkin S, Naylor S.
Detection and quantitation of cellularly derived
amyloid beta peptides by immunoprecipitation-HPLC-MS.
FEBS Lett. 1998 Jul 3;430(3):419-23.
PMID: 9688584 [PubMed - indexed for MEDLINE]
Adrenocortical actin
Purification of rat adrenocortical actin and its
use in an immunoprecipitation assay to quantitate cellular actin.
Biochim Biophys Acta. 1986 Sep 4;883(2):383-7.
PMID: 3527278 [PubMed - indexed for MEDLINE]
Adrenal microsomal antigen
Furmaniak J, Talbot D, Reinwein D, Benker G, Creagh FM, Rees Smith
B.
Immunoprecipitation of
human adrenal microsomal antigen.
FEBS Lett. 1988 Apr 11;231(1):25-8.
PMID: 3360129 [PubMed - indexed for MEDLINE]
beta-amyloid peptide
Portelius E, Westman-Brinkmalm A, Zetterberg H, Blennow K.
Determination of beta-amyloid peptide
signatures in cerebrospinal fluid using immunoprecipitation-mass
spectrometry.
J Proteome Res. 2006 Apr;5(4):1010-6.
PMID: 16602710 [PubMed - indexed for MEDLINE]
c-myb
Identification of c-myb (chicken), c-myb (mouse)
and v-myb (AMV) protein products by immunoprecipitation with
antibodies directed against a synthetic peptide.
FEBS Lett. 1986 Sep 1;205(1):104-8.
PMID: 3527747 [PubMed - indexed for MEDLINE]
Erythrocyte antigen
Laing P, Parkar BA, Culbert EJ, Watt GJ, Elson CJ.
Identification of rat erythrocyte antigens with a
new non-radioactive immunoprecipitation technique.
Scand J Immunol. 1987 Jun;25(6):613-20.
PMID: 3602935 [PubMed - indexed for MEDLINE]
Erythropoietin
Widness JA, Schmidt RL, Veng-Pedersen P, Modi NB, Sawyer ST.
A sensitive and specific erythropoietin
immunoprecipitation assay: application to pharmacokinetic studies.
J Lab Clin Med. 1992 Mar;119(3):285-94.
PMID: 1311741 [PubMed - indexed for MEDLINE]
Factor VIII
Detection of all anti-factor VIII antibodies in
haemophilia A patients by the Bethesda assay and a more sensitive
immunoprecipitation assay.
Haemophilia. 2001 Jan;7(1):26-32.
PMID: 11136377 [PubMed - indexed for MEDLINE]
Fonsecaea pedrosoi
Vidal MS, de Castro LG, Cavalecate SC, Lacaz Cda S.
Immunoprecipitation
techniques and Elisa in the detection of anti-Fonsecaea pedrosoi
antibodies in chromoblastomycosis.
Rev Inst Med Trop Sao Paulo. 2003 Nov-Dec;45(6):315-8. Epub 2004 Jan
26.
PMID: 14762630 [PubMed - indexed for MEDLINE]
GAD65
High prevalence of GAD65 (and IA-2) antibodies in
Japanese IDDM patients by a new immunoprecipitation assay based on
recombinant human GAD65.
Diabet Med. 1997 Sep;14(9):778-84.
PMID: 9300229 [PubMed - indexed for MEDLINE]
gamma-aminobutyric acid A receptor
Duggan MJ, Pollard S, Stephenson FA.
Quantitative immunoprecipitation studies with
anti-gamma-aminobutyric acidA receptor gamma 2 1-15 Cys antibodies.
J Neurochem. 1992 Jan;58(1):72-7.
PMID: 1309240 [PubMed - indexed for MEDLINE]
Growth hormone binding protein
Ho KK, Valiontis E, Waters MJ, Rajkovic IA.
Regulation of growth hormone binding protein in
man: comparison of gel chromatography and immunoprecipitation
methods.
J Clin Endocrinol Metab. 1993 Feb;76(2):302-8.
PMID: 8432772 [PubMed - indexed for MEDLINE]
HSP101
Co-immunoprecipitation of Hsp101 with cytosolic
Hsc70.
Plant Physiol Biochem. 2005 Jan;43(1):13-8. Epub 2005 Jan 21.
PMID: 15763661 [PubMed - indexed for MEDLINE]
Human platelet alloantigens and glycoproteins
Smith JW, Hayward CP, Warkentin TE, Horsewood P, Kelton JG.
Investigation of human platelet alloantigens and
glycoproteins using non-radioactive immunoprecipitation.
J Immunol Methods. 1993 Jan 14;158(1):77-85.
PMID: 8429218 [PubMed - indexed for MEDLINE]
IA-2
Masuda M, Powell M, Chen S, Beer C, Fichna P, Rees Smith B,
Furmaniak J.
Autoantibodies to IA-2 in
insulin-dependent diabetes mellitus. Measurements with a new
immunoprecipitation assay.
Clin Chim Acta. 2000 Jan 20;291(1):53-66.
PMID: 10612717 [PubMed - indexed for MEDLINE]
IgE
Kolmannskog S, Haneberg B, Florholmen J.
Detection of immunoglobulin E in feces by
immunoprecipitation, and characterization of associated
non-immunoglobulin precipitins.
Int Arch Allergy Appl Immunol. 1985;76(3):226-31.
PMID: 3918941 [PubMed - indexed for MEDLINE]
IgG
Human IgG subclass-specific rabbit antisera
suitable for immunoprecipitation in gel, ELISA and multilayer
haemagglutination techniques.
J Immunol Methods. 1985 Nov 28;84(1-2):203-20.
PMID: 2415635 [PubMed - indexed for MEDLINE]
Insulin receptor
Bailyes EM, Nave BT, Soos MA, Orr SR, Hayward AC, Siddle K.
Insulin receptor/IGF-I receptor hybrids are
widely distributed in mammalian tissues: quantification of
individual receptor species by selective immunoprecipitation and
immunoblotting.
Biochem J. 1997 Oct 1;327 ( Pt 1):209-15.
PMID: 9355755 [PubMed - indexed for MEDLINE]
Perrotti N, Taylor SI, Richert ND, Rapp UR, Pastan IH, Roth J.
Immunoprecipitation of
insulin receptors from cultured human lymphocytes (IM-9 cells) by
antibodies to pp60src.
Science. 1985 Feb 15;227(4688):761-3.
PMID: 3918346 [PubMed - indexed for MEDLINE]
Chvatchko Y, Van Obberghen E, Kiger N, Fehlmann M.
Immunoprecipitation of
insulin receptors by antibodies against Class 1 antigens of the
murine H-2 major histocompatibility complex.
FEBS Lett. 1983 Nov 14;163(2):207-11.
PMID: 6196229 [PubMed - indexed for MEDLINE]
Interferon (IFN)-beta
Serial immunoprecipitation assays for
interferon--(IFN)-beta antibodies in multiple sclerosis patients.
Eur Cytokine Netw. 2003 Jul-Sep;14(3):154-7.
PMID: 14656689 [PubMed - indexed for MEDLINE]
Interleukin-1 receptor
Rangnekar VM, Thomas LS, Plate JM.
Immunoprecipitation of interleukin-1 receptors
from a mouse thymoma cell line, EL-4.
J Biol Chem. 1988 Nov 5;263(31):16408-13.
PMID: 2972709 [PubMed - indexed for MEDLINE]
Interleukin-2 receptor
Schwarting R, Gerdes J, Ziegler A, Stein H.
Immunoprecipitation of the
interleukin-2 receptor from Hodgkin's disease derived cell lines by
monoclonal antibodies.
Hematol Oncol. 1987 Jan-Mar;5(1):57-64.
PMID: 3106185 [PubMed - indexed for MEDLINE]
Intestinal alkaline phosphatase
Lehmann FG, Cramer P, Hillert U.
Intestinal alkaline phosphatase: an
immunoprecipitation method for the determination in feces.
Clin Chim Acta. 1980 Aug 19;105(3):367-76.
PMID: 6996870 [PubMed - indexed for MEDLINE]
Lactose transporter
Quantitative immunoprecipitation of the lactose
transporter from Escherichia coli confirms stoichiometry of
substrate binding.
Biochem Biophys Res Commun. 1986 Jan 29;134(2):975-81.
PMID: 3511912 [PubMed - indexed for MEDLINE]
Lipoprotein lipase
Doolittle MH, Ben-Zeev O, Briquet-Laugier V.
Enhanced detection of lipoprotein lipase by
combining immunoprecipitation with Western blot analysis.
J Lipid Res. 1998 Apr;39(4):934-42.
PMID: 9555956 [PubMed - indexed for MEDLINE]
Lutropin receptor
Immunoprecipitation of the
lutropin receptor. Loss of receptor molecules during
down-regulation.
Biochem J. 1984 Dec 1;224(2):467-71.
PMID: 6097219 [PubMed - indexed for MEDLINE]
Malarial acid endopeptidase
Hempelmann E, Putfarken B, Rangachari K, Wilson RJ.
Immunoprecipitation of
malarial acid endopeptidase.
Parasitology. 1986 Apr;92 ( Pt 2):305-12.
PMID: 3520446 [PubMed - indexed for MEDLINE]
Melanocytes
Naughton GK, Eisinger M, Bystryn JC.
Detection of antibodies to melanocytes in
vitiligo by specific immunoprecipitation.
J Invest Dermatol. 1983 Dec;81(6):540-2.
PMID: 6196421 [PubMed - indexed for MEDLINE]
Muscle-specific receptor tyrosine kinase autoantibodies
Matthews I, Chen S, Hewer R, McGrath V, Furmaniak J, Rees Smith B.
Muscle-specific receptor tyrosine kinase
autoantibodies--a new immunoprecipitation assay.
Clin Chim Acta. 2004 Oct;348(1-2):95-9.
PMID: 15369741 [PubMed - indexed for MEDLINE]
Muscarinic receptor
Gil DW, Krauss HA, Bogardus AM, WoldeMussie E.
Muscarinic receptor
subtypes in human iris-ciliary body measured by immunoprecipitation.
Invest Ophthalmol Vis Sci. 1997 Jun;38(7):1434-42.
PMID: 9191607 [PubMed - indexed for MEDLINE]
Neuron-specific protein
De Graan PN, Dekker LV, Oestreicher AB, Van der Voorn L, Gispen WH.
Determination of changes in
the phosphorylation state of the neuron-specific protein kinase C
substrate B-50 (GAP43) by quantitative immunoprecipitation.
J
Neurochem. 1989 Jan;52(1):17-23.
PMID: 2521182 [PubMed - indexed for MEDLINE]
Neutrophil
Lucena-Fernandes F, Dalpe G, Dagenais P, Richard C, Calvert R, Boire
G, Menard HA.
Detection of anti-neutrophil cytoplasmic antibodies by
immunoprecipitation.
Clin Invest Med. 1995 Jun;18(3):153-62.
PMID: 7554581 [PubMed - indexed for MEDLINE]
Nicotinic acetylcholine receptor
Selective nicotinic acetylcholine receptor
subunit deficits identified in Alzheimer's disease, Parkinson's
disease and dementia with Lewy bodies by immunoprecipitation.
Neurobiol Dis. 2006 Aug;23(2):481-9.
Epub 2006 Jun 8.
PMID: 16759874 [PubMed - indexed for MEDLINE]
Onconeural antibodies
An immunoprecipitation assay for the detection
of onconeural antibodies.
Acta Neurol Scand Suppl.
2006;183:71-2.
PMID: 16637936 [PubMed - indexed for MEDLINE]
Opisthorchis viverrini antigen
Wongratanacheewin S, Chawengkirttikul R, Bunnag D, Sirisinha S.
Analysis of Opisthorchis viverrini antigens by
immunoprecipitation and polyacrylamide gel electrophoresis.
Parasitology. 1988 Feb;96 ( Pt 1):119-28.
PMID: 3362572 [PubMed - indexed for MEDLINE]
Parathyroid hormone receptor
Wright BS, Tyler GA, O'Brien R, Caporale LH, Rosenblatt M.
Immunoprecipitation of the
parathyroid hormone receptor.
Proc Natl Acad Sci U S A. 1987 Jan;84(1):26-30.
PMID: 3025860 [PubMed - indexed for MEDLINE]
PDE2
Immunoprecipitation of PDE2 phosphorylated and
inactivated by an associated protein kinase.
Methods Mol Biol. 2005;307:211-23.
PMID: 15988066 [PubMed - indexed for MEDLINE]
Phospholipase D
Immunoprecipitation of a
phospholipase D activity with antiphosphotyrosine antibodies.
J Interferon Cytokine Res. 1995 Oct;15(10):877-85.
PMID: 8564710 [PubMed - indexed for MEDLINE]
Placental alkaline phosphatase
Nickson DA, Livingstone JC, Sutcliffe RG.
Detection of the mRNA to placental alkaline
phosphatase by in vitro translation and immunoprecipitation.
Dis Markers. 1986 Dec;4(4):283-91.
PMID: 2898318 [PubMed - indexed for MEDLINE]
Protein kinase FA/glycogen synthase kinase 3
Van Lint J, Khandelwal RL, Merlevede W, Vandenheede JR.
A specific immunoprecipitation assay for the
protein kinase FA/glycogen synthase kinase 3.
Anal Biochem. 1993 Jan;208(1):132-7.
PMID: 8382016 [PubMed - indexed for MEDLINE]
Ri antibodies
Knudsen A, Monstad SE, Dorum A, Lonning PE, Salvesen HB, Drivsholm
L, Aarseth JH, Vedeler CA.
Ri antibodies in patients with breast, ovarian
or small cell lung cancer determined by a sensitive
immunoprecipitation technique.
Cancer Immunol Immunother. 2006
Oct;55(10):1280-4. Epub 2006 Jan 21.
PMID: 16429314 [PubMed - indexed for MEDLINE]
RNA
Schmitz-Linneweber C, Williams-Carrier R, Barkan A.
RNA immunoprecipitation and microarray analysis
show a chloroplast Pentatricopeptide repeat protein to be associated
with the 5' region of mRNAs whose translation it activates.
Plant Cell. 2005 Oct;17(10):2791-804. Epub 2005 Sep 2.
PMID: 16141451 [PubMed - indexed for MEDLINE]
Steroid 21-hydroxylase autoantibodies
Steroid 21-hydroxylase
autoantibodies: measurements with a new immunoprecipitation assay.
J Clin
Endocrinol Metab. 1997 May;82(5):1440-6.
PMID: 9141530 [PubMed - indexed for MEDLINE]
SV40 T-antigen
Use of sequential immunoprecipitation to reveal discrete, separable
populations of SV40 T-antigen binding to host cellular proteins.
J Virol Methods. 1996 May;59(1-2):105-12.
PMID: 8793836 [PubMed - indexed for MEDLINE]
TAR RNA-Binding Protein
Blair ED, Roberts CM, Snowden BW, Gatignol A, Benkirane M, Jeang KT.
Expression of TAR RNA-Binding Protein in
Baculovirus and Co-Immunoprecipitation with Insect Cell Protein
Kinase.
J Biomed Sci. 1995 Oct;2(4):322-329.
PMID: 11725069 [PubMed - as supplied by publisher]
Thyroid microsomal antigen
Kajita Y, Morgan D, Parkes AB, Rees Smith B.
Labelling and
immunoprecipitation of thyroid microsomal antigen.
FEBS Lett. 1985 Aug 5;187(2):334-8.
PMID: 3839463 [PubMed - indexed for MEDLINE]
Hamada N, Grimm C, Mori H, DeGroot LJ.
Identification of a thyroid microsomal antigen by
Western blot and immunoprecipitation.
J Clin Endocrinol Metab. 1985 Jul;61(1):120-8.
PMID: 3889034 [PubMed - indexed for MEDLINE]
Thyrotrophin receptor
Anti-thyrotrophin receptor antibodies in Graves'
disease as demonstrated directly by immunoprecipitation assay.
Acta Endocrinol (Copenh). 1983 Jan;102(1):49-56.
PMID: 6297216 [PubMed - indexed for MEDLINE]
TSH-receptor
De Bruin TW, Braverman LE, Brown RS.
Further evaluation of an immunoprecipitation
assay for TSH-receptor autoantibodies in Graves' disease.
Metabolism. 1986 Dec;35(12):1101-5.
PMID: 2878349 [PubMed - indexed for MEDLINE]
Konishi J, Iida Y, Kasagi K, Ikekubo K, Kuma K, Torizuka K.
Adipocyte-TSH-receptor-related
antibodies in Graves' disease detected by immunoprecipitation.
Endocrinol Jpn. 1982 Apr;29(2):219-26.
PMID: 6127208 [PubMed - indexed for MEDLINE]
Xenoestrogens
Natarajan K, Overstreet JW, Rogers JM, Denison MS, Chen J, Lohstroh
PN, McConnell DS, Lasley BL.
Detection of xenoestrogens in serum after
immunoprecipitation of endogenous steroidal estrogens.
Environ Health Perspect. 2002 Aug;110(8):791-5.
PMID: 12153760 [PubMed - indexed for MEDLINE]
Yo antibodies
Monstad SE, Storstein A, Dorum A, Knudsen A, Lonning PE, Salvesen
HB, Aarseth JH, Vedeler CA.
Yo antibodies in ovarian and breast cancer
patients detected by a sensitive immunoprecipitation technique.
Clin Exp Immunol. 2006
Apr;144(1):53-8.
PMID: 16542365 [PubMed - indexed for MEDLINE]