Immunoprecipitation (IP)

General Methods & Techniques

 

 

 

Filatov AV, Krotov GI, Zgoda VG, Volkov Y.

Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry.
J Immunol Methods. 2007 Jan 30;319(1-2):21-33. Epub 2006 Oct 13.
PMID: 17098248 [PubMed - indexed for MEDLINE]

 

Burbelo PD, Goldman R, Mattson TL.

A simplified immunoprecipitation method for quantitatively measuring antibody responses in clinical sera samples by using mammalian-produced Renilla luciferase-antigen fusion proteins.
BMC Biotechnol. 2005 Aug 18;5:22.
PMID: 16109166 [PubMed - indexed for MEDLINE]

 

Thompson S.

Immunoprecipitation and blotting: the visualization of small amounts of antigens using antibodies and lectins.
Methods Mol Med. 2004;94:33-45.
PMID: 14959821 [PubMed - indexed for MEDLINE]

 

O'Neill LP, Turner BM.

Immunoprecipitation of native chromatin: NChIP.
Methods. 2003 Sep;31(1):76-82.
PMID: 12893176 [PubMed - indexed for MEDLINE]

 

Klenova E, Chernukhin I, Inoue T, Shamsuddin S, Norton J.

Immunoprecipitation techniques for the analysis of transcription factor complexes.
Methods. 2002 Mar;26(3):254-9.
PMID: 12054881 [PubMed - indexed for MEDLINE]

 

Niranjanakumari S, Lasda E, Brazas R, Garcia-Blanco MA.

Reversible cross-linking combined with immunoprecipitation to study RNA-protein interactions in vivo.
Methods. 2002 Feb;26(2):182-90.
PMID: 12054895 [PubMed - indexed for MEDLINE]

 

Papavassiliou AG.

Preservation of protein phosphoryl groups in immunoprecipitation assays.
J Immunol Methods. 1994 Mar 29;170(1):67-73.
PMID: 8157989 [PubMed - indexed for MEDLINE]

 

Hartmann DJ, Grassi J, Ville G.

The effects of PEG on second antibody immunoprecipitation and its use in immunoassay.
J Immunoassay. 1993 Dec;14(4):241-66.
PMID: 8294568 [PubMed - indexed for MEDLINE]

 

Doolittle MH, Martin DC, Davis RC, Reuben MA, Elovson J.

A two-cycle immunoprecipitation procedure for reducing nonspecific protein contamination.
Anal Biochem. 1991 Jun;195(2):364-8.
PMID: 1750692 [PubMed - indexed for MEDLINE]

 

Ayres M, Marshall HB, Ishikawa J, Takahashi R.

Rapid preparation of lysate for immunoprecipitation using a low protein-binding micro-filter.
Biotechniques. 1991 May;10(5):578-80.
PMID: 1910772 [PubMed - indexed for MEDLINE]

 

Dedon PC, Soults JA, Allis CD, Gorovsky MA.

A simplified formaldehyde fixation and immunoprecipitation technique for studying protein-DNA interactions.
Anal Biochem. 1991 Aug 15;197(1):83-90.
PMID: 1952079 [PubMed - indexed for MEDLINE]

 

Kohn J, Riches PG, Raymond JC.

The use of alkaline-phosphatase conjugated second antibody for the enhancement of immunoprecipitation techniques on cellulose acetate membranes.
J Immunol Methods. 1985 Jan 21;76(1):11-6.
PMID: 3918117 [PubMed - indexed for MEDLINE]

 

Rush JS, Thorpe SR, Baynes JW.

A rapid micro-modification of the quantitative immunoprecipitation assay.
J Immunol Methods. 1983 Aug 26;62(2):247-55.
PMID: 6886434 [PubMed - indexed for MEDLINE]

 

Anderson DJ, Blobel G.

Immunoprecipitation of proteins from cell-free translations.
Methods Enzymol. 1983;96:111-20.
PMID: 6361451 [PubMed - indexed for MEDLINE]

 

LoPresti JS, Warren DW, Kaptein EM, Croxson MS, Nicoloff JT.

Urinary immunoprecipitation method for estimation of thyroxine to triiodothyronine conversion in altered thyroid states.
J Clin Endocrinol Metab. 1982 Oct;55(4):666-70.
PMID: 7050140 [PubMed - indexed for MEDLINE]