Western Blotting (WB)

General Methods & Techniques

 

 

 

Haan C, Behrmann I.

A cost effective non-commercial ECL-solution for Western blot detections yielding strong signals and low background.
J Immunol Methods. 2007 Jan 10;318(1-2):11-9. Epub 2006 Nov 2.
PMID: 17141265 [PubMed - indexed for MEDLINE]

 

Uhlig U, Uhlig S, Branscheid D, Zabel P, Vollmer E, Goldmann T.

HOPE technique enables Western blot analysis from paraffin-embedded tissues.
Pathol Res Pract. 2004;200(6):469-72.
PMID: 15310150 [PubMed - indexed for MEDLINE]

 

Wu M, Stockley PG, Martin WJ 2nd.

An improved western blotting technique effectively reduces background.
Electrophoresis. 2002 Aug;23(15):2373-6.
PMID: 12210190 [PubMed - indexed for MEDLINE]

 

Li W, Murai Y, Okada E, Matsui K, Hayashi S, Horie M, Takano Y.

Modified and simplified western blotting protocol: use of intermittent microwave irradiation (IMWI) and 5% skim milk to improve binding specificity.
Pathol Int. 2002 Mar;52(3):234-8.
PMID: 11972867 [PubMed - indexed for MEDLINE]

 

van Kooten TG, Klein CL, Kirkpatrick CJ.

Western blotting as a method for studying cell-biomaterial interactions: the role of protein collection.
J Biomed Mater Res. 2001 Mar 5;54(3):385-9.
PMID: 11189044 [PubMed - indexed for MEDLINE]

 

Korth MJ, Finn DJ, Gustafson GL.

Use of a Western blotting technique in the purification of a cysteine proteinase inhibitor.
Anal Biochem. 1988 Feb 15;169(1):181-4.
PMID: 3259407 [PubMed - indexed for MEDLINE]

 

Smith AJ, Cawston TE, Hazleman BL.

A rapid and reproducible method for the analysis of immune complexes using affinity chromatography and Western blotting.
J Immunol Methods. 1985 Nov 28;84(1-2):125-34.
PMID: 3877768 [PubMed - indexed for MEDLINE]

 

Dennis-Sykes CA, Miller WJ, McAleer WJ.

A quantitative Western Blot method for protein measurement.
J Biol Stand. 1985 Oct;13(4):309-14.
PMID: 4055807 [PubMed - indexed for MEDLINE]