8-OHdG (8-hydroxy-2'-deoxyguanosine) Antibody Review

 

 

 

Introduction

  • a sensitive biological marker of oxidative damage and stress. PMID: 17559562

  • a widely used biomarker of oxidative stress in research related to DNA, protein damage as well as lipid peroxidation. PMID: 17442641

  • a global, noninvasive biomarker of in vivo oxidative DNA damage. PMID: 17275687

  • one of the major oxidative DNA base products, is a commonly used biomarker of oxidative DNA damage. PMID: 12220951

  • was first reported in 1984 as a major form of oxidative DNA damage product by heated sugar, Fenton-type reagents and X-irradiation in vitro. PMID: 9439711

  • urinary 8-OHdG not only is a biomarker of generalized, cellular oxidative stress but might also be a risk factor for cancer, atherosclerosis and diabetes. PMID: 14687888

Normal Expression

  • Urinary 8-hydroxy-2'-deoxyguanosine excretion of the breast-fed group was significantly lower than those of the artificial milk dominant mixed-fed group or the bottle-fed group. PMID: 12621121

  • The expression of 8-OHdG in the human pituitary gland was investigated immunohistochemically using specimens from individuals aged 19-88 years. 8-OHdG was localized at the nuclei of the anterior lobar parenchymal cells of the pituitary gland. PMID: 12197673

  • 8-OH-dG levels in organs of young rats ranged between 10 and 90 micromol/mol deoxyguanosine (dG). Highest levels (micromol 8-OH-dG /mol dG) were detected in the oesophagus (90), muscle (61), brain (65), liver (59), spleen (57), and testicles (63). 8-OH-dG in DNA from kidney, lung, heart, small and large intestine ranged between 28 and 38 micromol/mol dG. Lowest amounts were found in the glandular stomach (10). DNA of old rats generally contained higher 8-OH-dG levels with significant increases in liver (186%) and kidney (372%); other organs showed no significant decreases (spleen, brain, testicles) or increases up to 69% (heart). PMID: 11602211

Abnormal Expression

  • Urinary 8-OHdG levels were significantly higher among breast cancer patients than among the control subjects, after making adjustments for confounders such as smoking, coffee consumption and use of oral contraceptives. PMID: 17512776

  • the excretion levels of urinary 8OHdG in cancer patients were significantly higher than those in healthy persons. PMID: 16260379

  • The total urinary 8-OHdG content was significantly higher in stroke patients than in non-stroke inpatients (p<0.01). PMID: 16157017

  • 8-OHdG levels in DNA from leukocytes of bladder cancer patients were significantly higher than those in controls. PMID: 14512647

  • There was a significant increase in serum 8-OHdG in haemodialysis (HD) patients compared with normal subjects. Serum 8-OHdG was positively correlated with the patients' age (r=0.231, P<0.05) but not with the duration of HD. Serum 8-OHdG was significantly higher in diabetic subjects than in non-diabetic subjects (P<0.05). PMID: 12686667

  • Baseline 8-OHdG levels were greater among subjects with rheumatoid arthritis (RA) compared to both healthy young (P < 0.001) and elderly (P < 0.05) subjects. There were no changes in 8-OHdG levels in either RA or elderly subjects as a result of the strength training intervention. PMID: 11137896

  • An immunohistochemical method using a monoclonal antibody specific for 8-OHdG showed stronger and more extensive staining in the nuclei of UV-irradiated epidermal cells than in those of nonirradiated cells. PMID: 8875958

  • Significantly higher levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG) were detected in a dose-dependent manner in epidermis of mice exposed to near-UV than in those of control animals. PMID: 8024554

Diagnostic & Therapeutic Value

  • levels of 8-OH-dG could predict survival in resected nonsmall-cell lung cancer (NSCLC) patients. PMID: 17154177

  • oxidative DNA damage is increased in patients with DCM according to the severity of HF. Serum levels of 8-OHdG may represent clinically useful markers of left ventricular remodeling. PMID: 16952786

  • Measurement of urinary 8-OHdG could be useful for evaluating in vivo oxidative DNA damage in mercury-exposed populations. PMID: 15695327

  • oxidative DNA damage is increased in association with necroinflammation in chronic liver disease and determination of 8-OHdG is useful in assessing high-grade malignancy in hepatocellular carcinoma (HCC). PMID: 14708894

  • 8-OHdG content in leukocyte DNA is a biomarker of oxidant-induced DNA damage in hemodialysis (HD) patients. PMID: 11054349

  • it might be possible to determine the spread of cancer to some extent by determining a patient's urinary 8-OHdG level. PMID: 8870419

  • The present results support 8-OHdG as a biomarker that may aid both in the diagnosis and in the documentation of progression in Parkinson's disease (PD). PMID: 17188662

  • it might be possible to determine the spread of cancer to some extent by determining a patient's urinary 8-OHdG level. PMID: 8870419

Review Articles

  • 8-Hydroxy-2'-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures. PMID: 16685565

Applications

 

ELISA

  • The level of 8-OHdG in each pool was subsequently determined using a commercial ELISA kit. Results confirmed that oligonucleotides containing 8-OHdG are present in urine and, most significantly, oligomers of <30-55 bases were found to be associated with 8-OHdG. PMID: 17275687

  • The 85 participants in our comparison group were matched on age, gender, and ethnicity to the depressed group. 8-OHdG was measured by enzyme-linked immunosorbent assay. PMID: 16449405. PMID: 16449405

  • To estimate the oxidative stress caused by radiotherapy, we used the enzyme-linked immunosorbent assay (ELISA) to monitor urinary 8-OHdG levels in 72 patients undergoing radiotherapy. PMID: 15809748

  • 8-hydroxy-2'-deoxyguanosine (8-OHdG), as a measure of oxidative stress, was measured in healthy Japanese volunteers using an ELISA (New 8-OHdG Check, JICA). PMID: 15630292

  • serum and urinary 8-OHdG levels were measured by using high-sensitive enzyme-linked immunosorbent assay (ELISA) kits. PMID: 15012720

  • 8-Hydroxy-2'-deoxyguanosine (8-OH-dG), which has been regarded as a potential marker of oxidative DNA damage induced by reactive oxygen species, was measured in human urine by a commercial ELISA using a monoclonal antibody N45.1 and by high-performance liquid chromatography (HPLC) coupled to an electrochemical detector (HPLC-ECD) to evaluate whether the ELISA system is applicable to human monitoring studies. PMID: 12163331

Immunofluorescence (IF)

  • We used immunofluorescence staining (monoclonal antibody N45.1) with cytological imprinting to study changes in the intranuclear distribution of 8-hydroxy-2'-deoxyguanosine in renal cells of male Wistar rats after oxidative stress by ferric nitrilotriacetate. PMID: 10330460

Immunohistochemistry (IHC)

  • We performed the immunohistochemical study of 8-OHdG in the brains of autopsied specimens. PMID: 12935688

  • we immunohistologically (LSAB method) demonstrated the presence of 8-hydroxy-2'-deoxyguanosine (8-OHdG) that was generated when oxidative DNA damage was caused by active oxygen species in noncancerous region obtained at hepatectomy for HCC, and investigated the relationship between 8-OHdG and remnant liver recurrence. PMID: 12699854

  • Immunohistochemical detection of 8-hydroxy-2'-deoxyguanosine in gallbladder epithelium of patients with pancreaticobiliary maljunction. PMID: 11692064

  • the 8-hydroxy-2'-deoxyguanosine (8-OHdG) level was assessed in human cervical cells by an immunoperoxidase method and was related to the presence of human papillomavirus (HPV) infection and precancerous lesions. PMID: 10837002

  • Normal human skin from three volunteers was exposed to UV radiation, and the time course of induction and removal of 8-OHdG was examined by immunohistochemical analysis with catalysed signal amplification on formalin-fixed paraffin sections. PMID: 10733270

  • We studied the presence of 8-hydroxy-2'-deoxyguanosine by immunohistochemistry using N45.1 monoclonal antibody in 28 cases of arsenic-related skin neoplasms and arsenic keratosis as well as in 11 cases of arsenic-unrelated Bowen's diseases. PMID: 10417614

  • Immunohistochemical detection of 8-hydroxy-2'-deoxyguanosine in paraffin-embedded sections of rat liver after carbon tetrachloride treatment. PMID: 9547863

  • Quantitative immunohistochemical determination of 8-hydroxy-2'-deoxyguanosine by a monoclonal antibody N45.1: its application to ferric nitrilotriacetate-induced renal carcinogenesis model. PMID: 9121119

  • 8-OHdG, indices of oxidative DNA damage, were measured by immunohistochemistry with diaminobenzidine detection in the brain, skeletal muscle, heart, liver, tenuum mucosa and lymphocytes from young (4 months) and aged (24 months) Sprague-Dawley rats fed ad libitum or held on two different caloric restriction diets (alternate day ad libitum feeding or daily feeding with 40% reduced calories). PMID: 15763395