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Immuno-Electron Microscopy (IEM) Methods, Techniques & Protocols
Definition: applying immunohistochemistry or immunocytochemistry method to localize antigens or proteins at sub-cellular level using an electron microscope.
Antigen-Antibody Specific Applications
Tissue-Cell Specific Applications
Tumor, Disease & Diagnostic Applications
TEM Immunogold Labeling Protocol - Pre-embedding Method (IHC World) - Detailed step by step procedure for EM immunogold staining technique on ultrathin sections.
TEM Immunogold Labeling Protocol - Post-embedding Method-1 (IHC World) - Detailed step by step procedure for EM immunogold staining technique on ultrathin sections.
TEM Immunogold Labeling Protocol - Post-embedding Method-2 (IHC World) - Detailed step by step procedure for EM immunogold staining technique on ultrathin sections.
Immunolabeling of thin sections for EM - Thin sections of biological material, mounted on specimen grids, can be easily labeled by floating them, section-side down, on small drops of antibody. This method will work for sections through resin-embedded material as well as for thin, thawed, cryosections.