Immunofluorescence (IF) Methods, Techniques, Protocols




Definition: labeling of antibodies or antigens with fluorescent dyes in cells or tissue sections which are visualized using a fluorescence or confocal microscope.

General Methods & Techniques

Multiple Labeling Methods & Techniques

Antigen-Antibody Specific Applications

Tissue-Cell Specific Applications

Virological Applications

Tumor, Disease & Diagnostic Applications

Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells - By Louise Cramer and Arshad Desai. We typically work with tissue culture, primary mammalian cells, and cell extracts, but the protocols can be adapted to other systems, such as whole embryos or lower eukaryotes.

Double Immunofluorescence Staining (IHC World) - By Giorgio Gattoretti. Any type of tissue is suitable for this technique, as long as the antigenicity for your antigen(s) is preserved.

Immunofluorescence Double Staining Method - Parallel Approach (IHC World) - This protocol includes preparation of slides, pretreatments, procedure, results, notes. This is a good protocol for two primary antibodies raised from different species.

Immunofluorescence Double Staining Method - Sequential Approach (IHC World) - This protocol can be used for two primary antibodies raised from the same species.

Autofluorescence (IHC World) - This page contains information about autofluorescence overview, and how to deal with autofluorescence using chemical methods, instrumentation methods as well as irradiation methods.